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Preparation carried with particles of antitumor drug and preparation method thereof

A technology of anti-tumor drugs and microparticles, which is applied in the direction of anti-tumor drugs, medical preparations of non-active ingredients, drug combinations, etc., can solve problems such as necrosis of mesenchymal stem cells, achieve improved curative effect, broad application prospects, and reduce toxic and side effects Effect

Inactive Publication Date: 2013-01-16
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is still no effective technology to solve the problem of mesenchymal stem cell necrosis caused by the accumulation of anti-tumor drugs and the transport of anti-tumor drug particles to malignant tumors and local sustained release of drugs

Method used

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  • Preparation carried with particles of antitumor drug and preparation method thereof
  • Preparation carried with particles of antitumor drug and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Formulation carrying doxorubicin hydrochloride microparticles

[0033] a. Preparation of antitumor drug particles: prepared by emulsification-solvent evaporation method. The preparation process is as follows: doxorubicin hydrochloride and PLA are dissolved in DMSO to form an organic phase. The above-mentioned organic phase is emulsified by ultrasound in the pH 7.4 phosphate buffer solution containing PVA205, and the organic solvent is quickly evaporated by drying under reduced pressure to prepare doxorubicin hydrochloride PLA with an average particle size of 120nm and a drug content of 2.5%. particle.

[0034] b. Preparation of multidrug-resistant mesenchymal stem cells expressing P-glycoprotein: using retrovirus as a vector, transfecting the multidrug resistance gene into mesenchymal stem cells to obtain multidrug-resistant mesenchymal stem cells expressing P-glycoprotein Stem cells, spare.

[0035] The preparation process is as follows: pMX-mdr1-GFP and o...

Embodiment 2

[0037] Example 2 Formulations carrying paclitaxel microparticles

[0038] a. Preparation of antitumor drug particles: prepared by emulsification-solvent evaporation method. The preparation process is as follows: dissolving paclitaxel and PLA in dichloromethane to form an organic phase. The above-mentioned organic phase is emulsified by ultrasound in a pH 7.4 phosphate buffer containing Tween 80, and the organic solvent is quickly evaporated by drying under reduced pressure to prepare paclitaxel PLA particles with an average particle size of 95nm and a drug content of 4.1%.

[0039] b. Preparation of P-glycoprotein-expressing multidrug-resistant mesenchymal stem cells: using lentivirus as a vector, the multidrug-resistant mesenchymal stem cells were transfected with multidrug resistance genes to obtain P-glycoprotein-expressing multidrug-resistant mesenchymal stem cells Stem cells, ready for use; the preparation process is as follows: using lipofection method, pMX-mdr1-GFP and...

Embodiment 3

[0041] Example 3 Formulations carrying docetaxel microparticles

[0042] a. Preparation of antitumor drug particles: prepared by emulsification-solvent evaporation method. The preparation process is as follows: docetaxel and PLGA are dissolved in acetone to form an organic phase. The above-mentioned organic phase is emulsified by ultrasonic wave in the pH 7.4 phosphate buffer solution containing Poloxamer 188, and the organic solvent is quickly evaporated in the mode of drying under reduced pressure. Cetaxel PLGA microparticles, wherein the molar ratio of propiolactone to acetolactone in PLGA is 1 / 99;

[0043] b. Preparation of multidrug-resistant mesenchymal stem cells expressing P-glycoprotein: using retrovirus as a vector, transfecting the multidrug resistance gene into mesenchymal stem cells to obtain multidrug-resistant mesenchymal stem cells expressing P-glycoprotein Stem cells, spare;

[0044] The preparation process is as follows: pMX-mdr1-GFP and outer membrane pro...

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Abstract

The invention provides a preparation carried with particles of antitumor drug and a preparation method thereof, belonging to the technical field of medicine. The preparation uses multidrug resistant mesenchymal stem cells as carrier of particles of antitumor drug, the multidrug resistant mesenchymal stem cells are multidrug resistant mesenchymal stem cells of a P-glycoprotein-expressing. According to the invention, the problem of necrocytosis of mesenchymal stem cells caused by antineoplastic drug accumulation is solved well, the particles of antitumor drug can be transported to a malignant tumor and released slowly in local, so that the targeted therapy of tumor is realized, curative effect is improved, toxic and side effect is reduced, and the invention has a wide application prospect.

Description

technical field [0001] The invention relates to a pharmaceutical preparation, in particular to a preparation loaded with antitumor drug particles, belonging to the technical field of medicine. Background technique [0002] Targeted therapy of malignant tumors has always been a hot spot of domestic and foreign medical workers. Mesenchymal stem cells (MSCs) are non-hematopoietic stem cells with multi-directional differentiation potential. In addition to the characteristics of hematopoietic support, immune regulation and multi-directional differentiation, they also have the ability to specifically migrate to injury sites and tumor tissues. characteristic. Recent studies have shown that mesenchymal stem cells can be transfected with adenovirus, retrovirus and lentivirus without changing the biological properties of mesenchymal stem cells. Therefore, tumor-targeted gene therapy using mesenchymal stem cells as cell carriers can specifically migrate to the primary tumor and metas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/46A61K9/14C12N5/10A61P35/00
Inventor 高子彬胡洁张丽男孙勇军申宁张莉李硕谢英花吴韶梅刘磊姚军杨恩芸
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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