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Cultivated fresh water microalgae and application thereof in production of biodiesel and nervonic acid

A technology of fresh water and microalgae, applied in the direction of microorganism-based methods, microorganisms, biofuels, etc., to achieve the effects of rich content, excellent culture cycle, and excellent oil content

Active Publication Date: 2011-10-19
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the cost problem has become a bottleneck problem restricting the industrialization of microalgae oil production. The main way to reduce the cost is through technological improvement and economical production. Attempts have not yet been resolved, so the breeding of energy microalgae that can be cultivated on a large scale, the cultivation of economically feasible energy microalgae and the development of high value-added products have become a powerful way to reduce production costs

Method used

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  • Cultivated fresh water microalgae and application thereof in production of biodiesel and nervonic acid
  • Cultivated fresh water microalgae and application thereof in production of biodiesel and nervonic acid
  • Cultivated fresh water microalgae and application thereof in production of biodiesel and nervonic acid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Isolation of Mychonastes sp.HSO-3-1 strain

[0031] Mychonastes sp.HSO-3-1 was isolated in the lotus root pool in the suburbs of Jinan City. The specific process was as follows: take an appropriate amount of water samples from the lotus root pool with a sterile bottle, and spread the BG-11 medium plate under aseptic conditions in the laboratory. Light incubator, cultivate at 25°C until algae colonies appear, pick multiple algae colonies and perform multiple streaking and separation steps until completely purified algae colonies are obtained. One of them, named HSO-3-1, was identified as a species of the genus Mychonastes by 18S rDNA and ITS sequence analysis.

[0032] Pick Mychonastes sp.HSO-3-1 single algae colony on solid plate under aseptic condition, pick the algae colony that obtains purifying through repeatedly streaking (applicant preserved described algae strain on March 4, 2011 In the General Microbiology Center of China Microbial Culture Collection M...

Embodiment 2

[0033] Embodiment 2: the initial cultivation of Mychonastes sp.HSO-3-1 algae liquid

[0034] Under laboratory conditions, the above-mentioned Mychonastes sp.HSO-3-1 was preserved as a test tube slant culture medium, or as a liquid culture medium in an Erlenmeyer flask, and the medium used was BG-11 medium. The activity of the preserved algae species is transferred in a cycle of 2 months. The algae liquid used for inoculation is generally at 200μmol photons m -2 the s -1 OD 750 Reach 2.0, inoculate to contain 50ml sterilized BG-11 culture fluid (NaNO 3 1500mg / L; K 2 HPO 4 40mg / L; MgSO 4 ·7H 2 O 75mg / L; CaCl 2 2H 2 O 36mg / L; Citric acid 6mg / L; Ferric ammonium citrate 6mg / L; Na 2 EDTA 1mg / L; NaCO 3 20mg / L;H 3 BO 3 2.86mg / L; MnCl 2 4H 2 O 1.81mg / L; ZnSO 4 ·7H 2 O 0.222mg / L; NaMoO 4 2H 2 O 0.39mg / L; CuSO 4 ·5H 2 O 0.079mg / L; Co(NO 3 ) 2 ·6H 2 O 0.049mg / L; deionized water 1L, adjust pH to 7.4) in a 100ml Erlenmeyer flask; then cultivate under continuous light...

Embodiment 3

[0035] The expanded culture of embodiment 3 Mychonastes sp.HSO-3-1 algae liquid and its use in the production of oil (the content of sodium nitrate is 0.15g / L)

[0036] The initial algae liquid that embodiment 2 obtains is inoculated in the sterilized BG-11 culture fluid of 250ml (K 2 HPO 4 40mg / L; MgSO 4 ·7H 2 O 75mg / L; CaCl 2 2H 2 O 36mg / L; Citric acid 6mg / L; Ferric ammonium citrate 6mg / L; Na 2 EDTA 1mg / L; NaCO 3 20mg / L;H 3 BO 3 2.86mg / L; MnCl 2 4H 2 O 1.81mg / L; ZnSO 4 ·7H 2 O 0.222mg / L; NaMoO 4 2H 2 O 0.39mg / L; CuSO 4 ·5H 2 O 0.079mg / L; Co(NO 3 ) 2 ·6H 2 (00.049mg / L; 1L of deionized water, adjust the pH to 7.4), wherein the content of sodium nitrate is 0.15g / L, insert the final concentration of cells OD 750 0.2, using a column culture device (diameter 42mm, length 600mm, wall thickness 2mm, material is ordinary glass, the same below), continuous light, light intensity 280 ~ 320μmol photons m -2 the s -1 , the temperature is 24 ℃ ~ 30 ℃, CO 2 The concen...

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Abstract

The invention discloses a microalgae strain Mychonases sp. HSO-3-1 and application thereof in the production of biodiesel and nervonic acid with high value. The invention also discloses growth conditions and grease content of the microalgae strain Mychonases sp. HSO-3-1 in different medium components. The microalgae strain Mychonases sp. HSO-3-1 can be used for producing multiple unsaturated fatty acids including linolenic acid C18: 3 and nervonic acid C24:1, with high added value; therefore, byproducts with high added value are obtained while biodiesel is obtained; and an environment-friendly production technology with low cost, which can be used for producing biodiesel and the byproducts with high added value, is supplied.

Description

Technical field: [0001] The present invention relates to microalgae, methods of screening for microalgae, and methods of utilizing algae to produce various products. Specifically, the present invention relates to a strain of freshwater microalgae Mychonastes sp.HSO-3-1, and the use of said strain for large-scale cultivation and for the production of oil, biodiesel, algae protein, fatty acid and / or raw application of material components. Background technique: [0002] Due to the energy crisis and global warming, coupled with human beings' emphasis on environmental protection issues, and the expectation of future fossil fuel price increases, it is urgent to develop a renewable bio-energy as a substitute, and to find a renewable green energy has become a scientific research Research hotspot of researchers, among which microalgae diesel is considered to be the most promising renewable green energy. Microalgae have high photosynthetic efficiency and do not compete with food cro...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P7/64C12P21/00C12P7/40C12R1/89
CPCY02E50/10
Inventor 李福利袁程刘君寒何茹
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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