Microalgae high-throughout culture equipment

Abstract

The invention provides microalgae high-throughout culture equipment comprising a frame, an oscillating mechanism, a temperature control device, a humidity control device, a ventilation control device, an objective table and a lighting device, wherein the objective table is suitable for fixing a culturing structure for containing an algae seed or algae liquid, the lighting device is arranged belowthe objective table and is suitable for providing even lighting for microalgae culture, the lighting device consists of a light source and a light guiding structure which comprises a light guiding layer, light reflection structures are arranged on the side surfaces and bottom surface of the light guiding layer so as to guide light from the light source to emit out from one surface of the light guide layer, therefore, light from the light source is evenly provided to the culturing structure containing the algae seed or algae liquid, and the lighting uniformity degree and lighting intensity formicroalgae high-throughout culture can be improved so as to meet the demands of microalgae growth and high-throughout screening.

Description

technical field [0001] The invention relates to the field of industrial application of microalgae, and in particular relates to equipment suitable for high-throughput cultivation of microalgae used in high-throughput screening of algae species. Background technique [0002] Microalgae are a kind of autotrophic plants widely distributed in land and ocean, rich in nutrients and high in photosynthetic utilization. Many microalgae can accumulate a large amount of oil in their cells. Some algae can contain up to 80% of the dry weight of the cell in the absence of nitrogen. Oil can be extracted from this type of microalgae by biorefinery for food preparation. oil and biodiesel; microalgae can also be used in industrial processes such as wastewater treatment to reduce environmental pollution. [0003] The environmental friendliness of microalgae, its health benefits to humans and animals, and its good application of bioenergy have triggered a global upsurge in research and develop...

AI Technical Summary

Problems solved by technology

[0006] At present, the laboratory system for high-throughput culture of algae species is relatively simple. The culture of algae strains is generally placed in an artificial climate box in a triangular flask system, with no ventilation or simply inserting a ventilation tube for ventilation, light and The temperature is regulated by the artificial climate box, and the temperature control is relatively uniform, but it is difficult to achieve consistent lighting and ventilation effects in this way

In addition, there is another culture system that is placed in a shaking table. Both the light and temperature are regulated by the shaking table, and the gas exchange is accelerated by shaking. Problems meeting the needs of algae growth and high-throughput screening

In addition, the above two methods also have the problems of large system and slow growth of microalgae.

[0007] Moreover, the existing light incubator or light shaker uses fluorescent tubes for light, which not only has problems such as uneven light and obvious light attenuation, but also has defects such as large heat production, short life, and discontinuous light adjustment.

Such a culture device is currently only suitable for culturing in a triangular flask. If it is used for culturing miniaturized experimental samples, these problems will become more prominent

In addition, the top lighting and container fixing methods of the traditional shaker also limit the use of microplates, especially in terms of ventilation, mixing, cross-contamination, evaporation and light, which cannot meet the implementation of miniaturized and high-throughput experimental techniques

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