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Radon gas and carbon monoxide gas united device for cell exposed contamination experiment

A technology of carbon monoxide and experimental equipment, applied in the direction of biochemical equipment and methods, biochemical instruments, biochemical cleaning devices, etc., can solve the problems of restricting the development of scientific research, lack of precise quantitative control, etc., and achieve small consumption of radon gas, convenient operation, The effect of precise quantitative control

Inactive Publication Date: 2011-10-12
SUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the existing experimental methods lack precise quantitative control, convenient operation, safety and no leakage, intuitive and reliable simulation and manufacture of special equipment for joint exposure of radon and carbon monoxide gas, and what is particularly lacking is the direct use of cells in vitro experiments Combined exposure to poisoning experiment equipment with carbon monoxide gas
Seriously restrict the development of scientific research in this area

Method used

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  • Radon gas and carbon monoxide gas united device for cell exposed contamination experiment
  • Radon gas and carbon monoxide gas united device for cell exposed contamination experiment

Examples

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Embodiment

[0025] When using the cell radon gas combined with carbon monoxide gas exposure exposure experimental equipment, first turn off the radon source 3 and the carbon monoxide generator 2, turn on the temperature controller 11, open the control room 7 and place the cell membrane plate 8 with the experimental cells planted in the cell exposure room 4, close the control room 7. Turn on the cell culture solution supplier 10, the radon source 3, the gas pump 1, and the carbon monoxide generator 2, and turn on and adjust the respective gas flow controllers to carry out the individual exposure or joint exposure poisoning experiment. When the radon gas is exposed to poison alone, only the radon source 3 is turned on, the gas pump 1 drives the radon gas and corresponding daughters to the cell exposure chamber 4 through the air inlet 5, and the flow controller is adjusted to expose the cells to the poisoned gas in the chamber 4 The flow rate is constant, and the mixed gas of predetermined c...

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Abstract

The invention provides a radon gas and carbon monoxide gas united device for a cell exposed contamination experiment. A radon source capable of preparing radon gas, a predetermined amount of radon gas which is generated by a carbon monoxide generator and decay products thereof and the carbon monoxide gas are conveyed into a cell exposing room through an air inlet; a radon and carbon monoxide gas united detector is used for monitoring the content of the contaminated gas; a temperature control room is connected with the temperature control room to guarantee that the temperature of the experiment environment is constant; a cell culturing liquid supplying device is connected with a culture liquid room to provide nutrients for the normal growth of the cell; the upper surface of a cell membranous lamina is exposed to the cell exposing room to carry out complete contamination; the experimental waste gas is recovered through an air outlet and can be recycled; the whole device is sealed and has no leakage. The beneficial effects are as follows: the invention provides the device for the cell exposed contamination experiment, which does not use experimental animals and can randomly adjust the contents of the radon gas and the carbon monoxide gas and the contamination combination order; and the invention provides a new method and a new experimental installation in the following scientific research fields such as toxicology, molecular biology, cytology, radioactivity, medical science and the like.

Description

technical field [0001] The invention relates to a cell radon gas combined with carbon monoxide gas exposure poisoning experiment equipment. Background technique [0002] Carbon monoxide is a colorless, odorless, non-irritating gas. It is a harmful substance that is often encountered in the human living environment. The main factor inducing lung cancer, and the harm to the human body produced by the combination of the two has been paid more and more attention to, and its research in the fields of toxicology, environmental protection, radiation, and medicine has become increasingly in-depth. However, the existing experimental methods lack precise quantitative control, convenient operation, safety and no leakage, intuitive and reliable simulation and manufacture of special equipment for combined exposure of radon and carbon monoxide gas, and what is particularly lacking is the direct use of cells in vitro experiments Combined with carbon monoxide gas exposure poisoning experim...

Claims

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Application Information

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IPC IPC(8): C12M1/34
Inventor 李建祥童建
Owner SUZHOU UNIV
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