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Antidepressant effect and application of effective parts of hypericum wightianum

A technology with effective parts and gold everywhere, applied in the field of antidepressant effect and application, to achieve the effect of obvious antidepressant effect

Inactive Publication Date: 2011-10-05
NANJING UNIV OF TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research is more in-depth, and the traditional Chinese medicine with better antidepressant effect is the plant of the genus Hypericum in the family Garcinia, but as a preparation, there are only a few preparations of Hypericum perforatum on the market abroad, and there are basically no plants of this genus in China. The finished preparation of the drug is launched

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0009] (1) The preparation method of the effective part of the gold

[0010] (1) Pulverize the dried medicinal material of Biandijin, degrease according to the conventional method, and extract the degreased medicinal material with a water-alcoholic liquid made of water and methanol, ethanol, propanol or n-butanol with an alcohol content of 0-80%, Add 2-5 times the amount of ethanol to the extract for precipitation.

[0011] (2) After filtering out the alcohol precipitate in the extract, concentrate the filtrate into a concentrate.

[0012] (3) The concentrated solution is separated by resin column chromatography, first washed with water to remove impurities, and then eluted with a water-alcoholic liquid gradient with an alcohol content of 10-95%, and the eluents are combined, concentrated, and dried under reduced pressure to obtain an effective part.

[0013] (2) Through the mouse tail suspension test, the mouse forced swimming test, the body temperature drop test caused by r...

Embodiment 1

[0015] Embodiment 1: the preparation of gold separation component

[0016] Grind 2kg of the dried medicinal material of Biandijin, degrease with 6 times the amount of petroleum ether, reflux and extract with 6 times the amount of 80% ethanol for 2 hours at a reflux temperature of 88-90°C, extract 3 times, filter, and combine the extracts. Recover the solvent under reduced pressure to obtain the total extract, add 3 times the amount of ethanol to the extract, filter out the alcohol precipitate in the extract, concentrate the filtrate into a concentrated solution, and use a D-101 macroporous The resin is separated and eluted with a gradient of 0-95% ethanol. Each gradient is water, 30% ethanol, 50% ethanol, 70% ethanol, and 95% ethanol. The water part is removed, and the other parts are collected and combined. The eluent is decompressed The solvent was recovered, and the residue was vacuum-dried to yield 41.5 g, which was the isolated fraction.

Embodiment 2

[0017] Example 2: Tail Suspension Experiment on Mice with Bidijin Separation Components

[0018] (1) Test animals: KM mice, male, weighing (20±2) g.

[0019] (2) Positive control drug: fluoxetine hydrochloride capsules.

[0020] (3) Male Kunming mice, 18-22g, 140, were randomly divided into 14 groups, 10 animals in each group, respectively normal control group, fluoxetine group and test drug large, medium and low dose groups. Animals in each group were administered intragastrically, and the normal control group was administered intragastrically with the same amount of normal saline. The mice in each group were given intragastric administration once a day for 7 consecutive days, and the diet and drinking water were not limited. One hour after the last administration, the mouse was glued to a horizontal wooden board (60 cm above the ground) at a place 2 cm from the tip of the tail, and the sight of the mouse was blocked by boards on both sides, and the accumulated immobility t...

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Abstract

The invention relates to an antidepressant effect and application of effective parts of hypericum wightianum. The extraction and separation method of the effective parts of the hypericum wightianum comprises the following steps: degreasing the hypericum wightianum; extracting with a water-alcohol liquid which is prepared from methanol, ethanol, propanol or n-butanol and water with alcohol amount of 0-80%; separating a concentrated solution with resin column chromatography; and carrying out antidepressant pharmacodynamic screening on various separation parts so as to find a separation component with a good antidepressant effect. In the specific embodiment, the component can obviously shorten the immobility time of mice in a tail suspension test and the immobility time of the mice in a forced swimming test, can obviously inhibit mice hypothermia caused by reserpine, and can obviously increase the evasion times of the mice in a learned helplessness test. The effective component of the hypericum wightianum has obvious antidepressant effect, can be used for preparing antidepressant preparations, or used for studying a specific compound in the component and developing antidepressant monomer compound preparations.

Description

technical field [0001] The invention relates to the technical field of Chinese herbal medicines, in particular to the extraction and separation of effective parts of the traditional Chinese medicine biandijin, and more specifically to the antidepressant effect and application of the effective parts of biandijin. Background technique [0002] Depression is the most common mental illness, and the lifetime incidence of depression in western industrialized countries is between 6% and 8%. With the development of social economy and the intensification of competitive pressure, the incidence of depressive symptoms and the prevalence of depression will become higher and higher, especially in the urban population. The overall scale of China's antidepressant drug market is still quite small, and it accounts for a very low proportion of the total pharmaceutical market. At present, the incidence rate of depression in China is 4.6%, which is comparable to the world level. Considering tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P25/24
Inventor 石金城孙兴华刘媛夏超祁超张桂森
Owner NANJING UNIV OF TECH
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