Method for inducing and differentiating functional cardiocytes by utilizing endometrial stem cells

Abstract

The invention discloses a method for inducing and differentiating functional cardiocytes by utilizing endometrial stem cells. The method comprises the following steps of: 1) performing separation culture on the endometrial stem cells and amplifying; and 2) inducing and differentiating in vitro, namely digesting 4th to 6th generation of cells in good growth condition, which is obtained in step 1) by using pancreatin, terminating the digestion by using a Dulbecco modified eagle medium (DMEM) containing 13 to 17 volume percent fetal calf serum when the cells are changed into single circular shapes under a microscope, centrifuging, washing an obtained cell precipitate by using phosphate buffer solution (PBS), adding an induction culture medium into the cells washed by the PBS, putting into anincubator which contains 4 to 6 percent of CO2 and has the saturated humidity of between 94 and 96 percent at the temperature of between 36 and 38 DEG C, and culturing to obtain the functional cardiocytes, wherein the culture time is 68 to 76 hours. The obtained functional cardiocytes can be used for treating myocardial infarction.

Description

technical field [0001] The invention relates to a method for using endometrium stem cells to induce differentiation of functional cardiomyocytes. Background technique [0002] At present, the incidence of congestive heart failure is increasing year by year. There are 15 million patients worldwide, with 465,000 new cases each year. It is the leading cause of hospitalization for people over 65 years old and the leading cause of cardiovascular death. Cell transplantation therapy using stem cells as a seed resource can promote angiogenesis and myocardial regeneration in the infarct area, thereby improving cardiac function after myocardial infarction, and has become a research hotspot in the cardiovascular field. However, embryonic cardiomyocytes available for transplantation have limited proliferative ability and inconvenient materials, and skeletal muscle myoblasts cannot form gap junctions with cardiomyocytes and can cause arrhythmia, which makes this treatment method stagnant...

AI Technical Summary

Problems solved by technology

[0004] 1. Insufficient potential to differentiate into functional cells such as cardiomyocytes and neuron cells;

[0005] 2. Most of the cells died after transplantation;

[0006] 3. The curative effect of transplantation is not satisfactory;

[0007] 4. Cell Number Limitation

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