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Method for culturing marine oil-producing microalgae

A technology of oil-producing microalgae and cultivation method, which is applied to the high-density cultivation of marine oil-producing microalgae and the production of oil-rich marine microalgae biomass, can solve the problem of increasing the production cost of oil-rich microalgae and the cultivation density of marine microalgae. problems such as low energy consumption, increased energy input, etc., to achieve the effect of simplifying the accumulation process, low cost, and promoting accumulation

Inactive Publication Date: 2011-09-21
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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Problems solved by technology

[0003] So far, the general problems of low culture density and high cost of marine microalgae have not been well resolved; in addition, the "two-step method" is commonly used to induce microalgae to accumulate a large amount of oil due to nitrogen deficiency. After harvesting by centrifugation, the microalgae were resuspended in nitrogen-deficient medium to induce oil production, adding additional energy input and increasing the production cost of oil-rich microalgae

Method used

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  • Method for culturing marine oil-producing microalgae
  • Method for culturing marine oil-producing microalgae
  • Method for culturing marine oil-producing microalgae

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Embodiment 1

[0026] Step 1: Optimization of the proportion of domestic sewage. Based on natural seawater, add 10%, 30%, 50%, 80% and 100% of domestic sewage after secondary treatment and filtration, inoculate with fresh Nannochloropsis, and use conventional f / 2 culture medium as a control. At a temperature of 25°C and a light intensity of 70 μmolm -2 the s -1 Under the conditions of static culture for 6 days. refer to figure 1 , the results of many tests show that the more suitable proportion of domestic sewage is 30% to 50%, and the biomass accumulated by Nannochloropsis within this range is 1.2 to 1.6 times that of the control medium.

[0027] Step 2: using domestic sewage and high-concentration carbon dioxide to cultivate Nannochloropsis. Add 50% domestic sewage in natural seawater as a medium, add fresh Nannochloropsis algae, and feed 15% CO2 after 24 hours 2 Culture was carried out until the growth of Nannochloropsis cells entered the middle and late stages of logarithmic growth...

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Abstract

The invention discloses a method for culturing marine oil-producing microalgae, which is characterized in that: the marine microalgae are culture by using daily sewage to supply nutritional salts and using high-concentration carbon dioxide to provide a carbon source, so the biomass yield of the marine microalgae is improved; and the microalgae are subjected to direct high-light exposure so as to improve the oil and fat yield of the marine microalgae. In the invention, the culture cost of the oil-enriched marine microalgae is lowered, the process of inducing the oil and fat accumulation in microalgae is simplified, and the industrialization of the large-scale culture of oil-enriched marine microalgae is promoted.

Description

technical field [0001] The invention relates to microalgae cultivation technology, in particular to a high-density cultivation method of marine oil-producing microalgae, which can be widely used in the production of oil-rich marine microalgae biomass. Background technique [0002] Microalgae are a solar-driven cell factory that absorbs CO through efficient photosynthesis 2 , convert light energy into chemical energy of compounds such as fat or starch, and release O 2 . The use of microalgae biomass to produce biodiesel can effectively solve the raw material bottleneck of the biofuel industry and provide a new development path for the healthy, stable and sustainable development of the bioenergy industry. Microalgae are photoautotrophic aquatic organisms. Microalgae cultivation requires a large amount of water. If microalgae are cultivated in a track pond, 1.1 to 1.3×10 per hectare (ha) per year is required. 7 liters of water. The ocean area on the earth accounts for 71% o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 郭荣波蒋礼玲罗生军杨智满范晓蕾
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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