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Method for preventing and treating fruit tree meloidogynosis by adopting carboxymethyl chitosan protective film

A technology of carboxymethyl chitosan and protective film, which is applied in the field of new biopesticides, can solve the problems of large side effects and poor effects, and achieve the effects of strong antibacterial ability, high bioavailability, and promoting wound healing

Inactive Publication Date: 2011-09-21
刘福京
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention focuses on the research and development of a carboxymethyl chitosan protective film that can both straighten Agrobacterium tumefaciens and protect wounds and promote wound healing. Bad question

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The technical scheme of the present embodiment is:

[0013] The first step: according to the weight ratio of water, citric acid, sodium hydroxide, chitosan oligosaccharide citrate 100:8:4:0.5, put citric acid, chitosan oligosaccharide citrate, and sodium hydroxide into water in sequence , to make a buffer with a pH value of <5, which is A solution.

[0014] The second step: according to the weight ratio of water, potassium iodide, iodine, and diiodotyrosine in the ratio of 100:0.3:0.15:0.01, put potassium iodide, iodine, and diiodotyrosine into the water in sequence, and heat while stirring. The temperature is 50°C, and it dissolves until transparent, which is B solution.

[0015] Step 3: Put carboxymethyl chitosan in water at a ratio of 100:4 by weight of water and carboxymethyl chitosan, heat at 60° C., stir to dissolve it, and form C solution.

[0016] Step 4: According to the weight ratio of water and sodium alginate of 100:0.01, the water is heated to 60°C, and t...

Embodiment 2

[0020] The technical scheme of the present embodiment is:

[0021] The first step: according to the weight ratio of water, citric acid, sodium hydroxide, chitosan oligosaccharide citrate 100:12:6:2, put citric acid, chitosan oligosaccharide citrate, and sodium hydroxide into water in sequence , to make a buffer with a pH value of <5, which is A solution.

[0022] The second step: according to the weight ratio of water, potassium iodide, iodine, and diiodotyrosine in the ratio of 100:0.8:0.3:0.02, put potassium iodide, iodine, and diiodotyrosine into the water in sequence, and heat while stirring. The temperature is 70°C, and it dissolves until transparent, which is B solution.

[0023] Step 3: Put carboxymethyl chitosan in water at a ratio of 100:6 by weight of water and carboxymethyl chitosan, heat at 80° C., stir to dissolve it, and form C solution.

[0024] Step 4: According to the weight ratio of water and sodium alginate of 100:0.03, heat the water to 80°C, and put the ...

Embodiment 3

[0028] The technical scheme of the present embodiment is:

[0029] The first step: according to the weight ratio of water, citric acid, sodium hydroxide, chitosan oligosaccharide citrate 100:10:4:1, put citric acid, chitosan oligosaccharide citrate, and sodium hydroxide into water in sequence , to make a buffer with a pH value of <5, which is A solution.

[0030] The second step: according to the weight ratio of water, potassium iodide, iodine, and diiodotyrosine in the ratio of 100:0.5:0.2:0.1, put potassium iodide, iodine, and diiodotyrosine into the water in sequence, and heat while stirring. The temperature is 70°C, and it dissolves until transparent, which is B solution.

[0031] Step 3: Put carboxymethyl chitosan in water at a ratio of 100:5 by weight of water and carboxymethyl chitosan, heat at 80° C., stir to dissolve it, and form C solution.

[0032] Step 4: According to the weight ratio of water and sodium alginate of 100:0.01, heat the water to 80°C, and put the s...

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Abstract

The invention discloses a method for preventing and treating fruit tree meloidogynosis by adopting a carboxymethyl chitosan protective film, and relates to a formula of a novel biological pesticide for preventing and treating fruit tree meloidogynosis and a preparation method of the novel biological pesticide. The preparation method comprises the steps of: preparing solution A in the weight ratio of water to citric acid to sodium hydroxide to chitosan oligosaccharide citrate of being 100: (8-12): (4-6): (0.5-2); preparing solution B in the weight ratio of water to potassium iodide to iodine to bioactive organic iodine of being 100: (0.3-0.8): (0.15-0.3): (0.01-0.02); preparing solution C in the weight ratio of water to carboxymethyl chitosan of being 100: (4-6); preparing solution D in the weight ratio of water to sodium alginate of being 100: (0.01-0.03); and finally, adding the solution B, the solution C and the solution D into the solution A in sequence. The novel biological pesticide can be used for effectively protecting wounds and promoting wound healing when the root systems of fruit trees have wounds because of grafting, meloidogynosis tumor removal and the like.

Description

(1) Technical field [0001] The invention relates to a formula for preventing and treating Agrobacterium tumefaciens in fruit trees by using a novel biological pesticide carboxymethyl chitosan protective film and a preparation method thereof. (2) Background technology [0002] Fruit tree root cancer (growngall of fruit trees), also known as crown gall, is a worldwide stubborn disease caused by Agrobacterium tumefaciens (Agrobactium spp.). This fungus has a wide range of hosts and can infect 643 species of plants belonging to 331 genera in 93 families, causing very large losses in production. Agrobacterium tumefaciens showed a negative Gram staining reaction and was suitable for growth in alkaline soils. The optimum pH value was PH7.3, and the range of acid and alkali resistance was PH5.7-9.2. It is recognized by the international academic community that all rhizobacteria use wounds as the only way of invasion, and they all use the same pathogenic mechanism to cause plant dis...

Claims

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Application Information

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IPC IPC(8): A01N59/12A01N59/00A01N43/16A01N37/44A01N37/36A01P3/00A01P21/00
Inventor 刘福京
Owner 刘福京
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