Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Joint detection kit for alpha,beta-thalassemia associated mutant genes

A technology for thalassemia and mutant genes, applied in the field of alpha, which can solve problems such as alpha that have not yet appeared

Active Publication Date: 2011-09-14
潮州凯普生物化学有限公司 +1
View PDF6 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] So far, no α, β-thalassemia synchronous joint detection product has been clinically available

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Joint detection kit for alpha,beta-thalassemia associated mutant genes
  • Joint detection kit for alpha,beta-thalassemia associated mutant genes
  • Joint detection kit for alpha,beta-thalassemia associated mutant genes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0064] 1. Preparation of gene chip for detection of α, β-thalassemia

[0065] 1-1. Probe design

[0066] α-thalassaemia is commonly seen in three α-globin gene deletions (-- SEA , -α 4.2 , -α 3.7 ) and 2 mutant types (Hb QS, Hb CS); β-thalassaemia is commonly found in 16 β-globin gene mutations (-28(A-G), -29(A-G), Cap(-AAAC), Int(T-G ), CD14 / 15(+G), CD17(A-T), βE(G-A), CD27 / 28(+C), CD31(-C), CD41 / 42(-TTCT), CD43(G-T), CD71 / 72 (+A), IVS-I-1(G-T, G-A), IVS-I-5(G-C), IVS-II-654(C-T)), in order to detect the above common mutation types of α, β-thalassaemia, this The inventors designed 31 probes in total, and the specific probes are SEQ ID Nos: 1-31.

[0067] 1-2. Spotting and Immobilization of DNA Probes

[0068] (1) Spotting and arrangement of probes

[0069] In the immobilization of direct oligonucleotide DNA probes, the DNA probes were first diluted with probe diluent (0.5M Na at pH 8.4 2 CO 3 and 0.5M NaHCO 3 solution) mixed and sampled. After the synthesis of the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a joint detection kit for detecting alpha,beta-thalassemia associated with mutant genes, which comprises (1) a gene chip and (2) a primer, wherein the gene chip is provided with probes, and the probes are a sequence SEQ ID Nos:1-31 and a sequence which is complementary to the sequence SEQ ID Nos:1-31; and the primer is a sequence SEQ ID Nos:32-42. The thalassemia gene detection kit provides a platform for detecting 16 mutant genes associated with alpha-thalassemia (three deletion types and two mutant types) and beta-thalassemia, can perform synchronous joint detection,improve the specificity of detection, reduce cost and shorten detection time, and has a great significance for the screening of patients suffering from thalassemia, genetic counseling and prenatal diagnosis.

Description

technical field [0001] The invention relates to the field of genes, in particular to a combined detection kit for α, β-thalassemia mutation genes. Background technique [0002] Thalassemia (hereinafter referred to as thalassemia) is a hemolytic anemia caused by an imbalance in the synthesis rate of α, β-globin peptide chains due to human gene mutation or deletion. Thalassemia is one of the most common genetic diseases in the world, and it is widely distributed in Mediterranean countries and other areas where malaria used to be high. In my country, the provinces south of the Yangtze River have a high incidence rate. In addition to the highest incidence rates in Guangdong, Guangxi, and Hainan provinces, Guizhou, Yunnan, and Sichuan are also high-incidence areas. The two common types of thalassemia are alpha-thalassemia and beta-thalassemia. [0003] α-thalassemia is a chronic hemolytic disease caused by autosomal genetic defect, which causes the loss or dysfunction of α-glob...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 朱娟娟李烈军邱美兰
Owner 潮州凯普生物化学有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com