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Application of active ingredients of juncus effuses in preparation of medicaments for resisting tumors or inhibiting angiogenesis, health-care food or cosmetics

A technology for tumor angiogenesis and effective components

Active Publication Date: 2013-02-27
JIANGSU SUDA INVESTMENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to literature research and novelty search, the effect of Juncus effuses L extract on tumor cells and tumor angiogenesis has not been reported in the literature at home and abroad so far.

Method used

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  • Application of active ingredients of juncus effuses in preparation of medicaments for resisting tumors or inhibiting angiogenesis, health-care food or cosmetics
  • Application of active ingredients of juncus effuses in preparation of medicaments for resisting tumors or inhibiting angiogenesis, health-care food or cosmetics
  • Application of active ingredients of juncus effuses in preparation of medicaments for resisting tumors or inhibiting angiogenesis, health-care food or cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Ethanol extract and water extract of Rush rush. Preparation: Dried Rush rush material, crushed, extracted twice with 5-10 times the amount of 95% ethanol or water cold soaking, each time for 24 hours, combined extracts, filtered, and reduced pressure Concentrate to obtain a thick extract, and dry it in an oven at 65°C.

Embodiment 2

[0040] Example 2: Dried rushes, crushed, extracted by cold immersion in 90% ethanol, the alcohol solution was concentrated under reduced pressure until it had no alcohol smell, let it cool to obtain a thick extract, suspended with water, filtered, and applied to the processed AB-8 macroporous resin column, eluted with water, 40% ethanol, and 80% ethanol in sequence, collected 90% ethanol eluted parts, recovered to dryness under reduced pressure, mixed with silica gel, loaded on silica gel (200 mesh) column, petroleum Ether-ethyl acetate 3:1 was eluted until blue fluorescence appeared in the eluent, all the light yellow fractions were combined, concentrated under reduced pressure until the precipitate was precipitated, filtered, and the filtrate was washed with petroleum ether-ethyl acetate 3 : 1 Repeatedly concentrate the precipitates, combine the precipitates to obtain the effective components of the rush.

Embodiment 3

[0041] Example 3: Effects of the effective components of Rush rush on the proliferation of various tumor cells:

[0042] 1 tumor cell line

[0043] Human Burkitt's lymphoma cells Namalva and Raji, pre-tumor stem cells 2C4G2, PMKO102-3# and p3B11, human T lymphocytic leukemia cell Jurkat, human T lymphocyte line H9, human breast cancer cell line MCF-7, human leukemia cell line UC -CUC, NB4 and HL-60, human osteosarcoma cell lines U-2OS and SW1353, human melanoma cell line C8161, human lung cancer cells (lymph node metastasis) NCI-H292, human gastric cancer cell line HGC-27, human liver cancer cell line HepG2 and human colon cancer cell line SW480 were cryopreserved in our laboratory.

[0044] Cell culture medium RPMI 1640 and DMEM (high sugar) are products of Hyclone Company. Alamarblue reagent is the product of INVITROGEN company.

[0045] 2 test drug

[0046] The ethanol extract of Rush rushes (prepared according to the method in Example 1, containing active components) i...

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PUM

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Abstract

The invention discloses application of active ingredients of juncus effuses in the preparation of medicaments for resisting tumors or inhibiting angiogenesis, health-care food or cosmetics. In the invention, the active ingredients of juncus effuses has the activity of inhibiting the tumors and tumor angiogenesis, and the prepared medicaments for inhibiting the tumors and the tumor angiogenesis can be used for treating or preventing malignancy and diseases relevant to the tumor angiogenesis and also can be used for treatment in tumor chemotherapy and / or auxiliary chemotherapy. In the application, the medicinal curative effect and effect mechanism of the active ingredients of juncus effuses are studied and clarified by taking tumor stem cells, tumor cells and tumor neoangiogenesis as target spots for treating diseases so as to establish a foundation for the research and development of the active ingredients of juncus effuses and the innovative anti-tumor traditional Chinese medicines of the active ingredients and provide scientific basis and important information for the Chinese medicinal treatment of the malignancy.

Description

technical field [0001] The invention relates to the application of the effective components of rushes in the preparation of antitumor drugs and tumor angiogenesis inhibitors, health food or cosmetics. Background technique [0002] According to statistics from the Ministry of Health in February 2010, the number of cancer deaths worldwide reaches 10 million each year. At present, one-quarter of the global population's death is caused by cancer, and it is predicted that if the current trend continues, one-half of the deaths will be due to cancer by 2050. The incidence of malignant tumors in our country is increasing at a rate of 2.5-5% per year, and has become the culprit that seriously endangers human life. Traditional Chinese medicine has a long history of treating tumors. So far, yew, camptophylla, arsenic, green tea, ganoderma lucidum, ginseng, periwinkle, asparagus, barbata, geranium, etc. have been used in clinical treatment of cancer, and some good results have been ach...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/88A61K8/97A23L1/29A61P35/00A61P35/02A61P27/02A61P17/06A61P19/02A61P9/14A61P9/10A61P17/00A61P17/10A23L33/00
Inventor 周泉生顾振纶蒋小岗曹志飞朱峰妍郭次仪
Owner JIANGSU SUDA INVESTMENT CO LTD
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