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Detection method for solid phase competition ELISA (Enzyme Linked Immuno Sorbent Assay) of foot and mouth disease

A detection method and solid-phase competition technology, applied in the field of serological diagnosis of animal epidemics, can solve problems such as complex operation

Inactive Publication Date: 2011-09-07
YUNNAN ANIMAL SCI & VETERINARY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For the above situation, the purpose of the present invention is to solve the problem of complex operation in the FMD solid-phase competition ELISA

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Preparation of FMD antigen immobilized plate:

[0018] After the standard rabbit anti-foot-and-mouth disease virus antibody was diluted proportionally with carbonic acid-sodium bicarbonate buffer solution of pH 9.6, 50 microliters per well was added to the wells of a 96-well ELISA plate, and after slight shaking, it was left overnight at 4°C. Discard the liquid in the wells the next day, wash with ELISA special washing solution (phosphate buffer, pH7.2, 0.05% Tween 20) for 3 times, add standard foot-and-mouth disease antigen at 50 microliters per well, and incubate at 37°C for 60 minutes Discard the liquid in the plate, wash 3 times with ELISA special washing solution, add 50 microliters of stabilizer to each well, place at 4°C overnight, control to dry and then vacuum pack. The packaged ELISA plate should be stored at 4°C for use, and can be stored at -20°C for long-term storage.

[0019] The use of FMD antigen immobilized plate:

[0020] Unpack the foot-and-mouth di...

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Abstract

The invention relates to a detection method for solid phase competition ELISA (Enzyme Linked Immuno Sorbent Assay) of foot and mouth disease, belonging to the technical field of serology diagnosis of animal epidemic diseases. The detection method comprises the following steps of: bonding a foot and mouth disease antibody on an ELISA plate through a coating technology; then firmly binding the foot and mouth disease antibody into the ELISA plate through an antigen-antibody binding reaction; processing and drying the foot and mouth disease antibody with a stabilizing agent and packaging the foot and mouth disease antibody in vacuum; and finally placing the foot and mouth disease antibody at low temperature for storage in a long period of time. When the foot and mouth disease antibody is necessary to detect, the ELISA plate can be directly used for experiments and can ensure that the experiment process is simple and saves time.

Description

technical field [0001] The invention relates to a detection method for foot-and-mouth disease solid-phase competitive ELISA, which belongs to the technical field of serological diagnosis of animal diseases. technical background [0002] Foot-and-mouth disease is a Class I disease identified by the International Veterinary Bureau, which has a serious impact on the economy of the affected area. The detection of the antibody level of foot-and-mouth disease is of great significance for vaccine immunity and disease control. [0003] ELISA is the abbreviation of enzyme linked immuno-sorbent assay, that is, enzyme-linked immunosorbent assay, which is an important method for the detection of foot-and-mouth disease antibodies today. This method is divided into liquid-phase blocking ELISA, namely LPB-ELISA (liquid phase block, LPB), Competitive ELISA with solid phase, namely SPC-ELISA (soil phase competition, SPC). Among them, SPC-ELISA is gradually becoming the mainstream FMD antibo...

Claims

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Application Information

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IPC IPC(8): G01N33/543
Inventor 李华春李乐苗海生廖德芳高林信爱国胡骑
Owner YUNNAN ANIMAL SCI & VETERINARY INST
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