Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of three-dimensional histioid cardiac muscular tissue for studying simulated microgravity effect

A microgravity-simulating, three-dimensional technology that is applied in the field of preparation of three-dimensional myocardium-like tissue can solve problems such as being easily disturbed by shear force, difficulty in harvesting cells, and large cell volume, and achieve the effect suitable for space loading

Inactive Publication Date: 2011-09-07
HARBIN INST OF TECH
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problem that cytodex microcarrier materials are toxic and have adverse effects on cardiomyocytes in the three-dimensional cell culture method currently used to study the simulated microgravity effect. The amount of cells required for culture is large, and it is difficult to collect cells. It is easy to be disturbed by shear force during culture, and the continuous contraction time of cardiomyocytes is short. Provide a method for preparing three-dimensional myocardial tissue-like microspheres for studying the effect of simulating microgravity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of three-dimensional histioid cardiac muscular tissue for studying simulated microgravity effect
  • Preparation method of three-dimensional histioid cardiac muscular tissue for studying simulated microgravity effect
  • Preparation method of three-dimensional histioid cardiac muscular tissue for studying simulated microgravity effect

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0015] Specific Embodiment 1: In this embodiment, a method for preparing a three-dimensional myocardium-like tissue for simulating microgravity effects is carried out according to the following steps: 1. A sodium alginate solution with a mass concentration of 0.05% to 3% is mixed with a mass concentration of Mixing 10% to 50% type I collagen solution to obtain a mixed solution; 2. Place the heart of a Wistar rat suckling mouse 1 to 3 days after birth in a 0.1mol / L PBS solution at 0 to 4°C, and then Wash the heart with 0.1mol / L PBS solution at 0-4°C for 3-5 times, then cut the heart tissue into 1-3mm 3 Tissue pieces of different sizes were washed with 0.1mol / L PBS solution at 0-4°C for 3-5 times, then digested with digestive juice for 10-30min, then added DMEM medium 10 times the volume of the tissue pieces, centrifuged Discard the supernatant, then add DMEM medium to the precipitate to make cell suspension A, inoculate cell suspension A into a culture bottle, adhere to the wal...

specific Embodiment approach 2

[0018] Embodiment 2: This embodiment is different from Embodiment 1 in that: the mass concentration of the sodium alginate solution in step 1 is 0.5%-2%. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0019] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the mass concentration of the sodium alginate solution in step 1 is 1%. Others are the same as in the first or second embodiment.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to a preparation method of a three-dimensional histioid cardiac muscular tissue for studying a simulated microgravity effect, in particular relating to a preparation method of a three-dimensional histioid cardiac muscular tissue microballoon, aiming at solving the problems that according to the current cell three-dimensional culture method for studying the simulated microgravity effect, the cytodex microcarrier material is toxic and is not conducive to generation of cardiocytes, a great number of cells are needed for culture, cell harvesting is difficult, the cells are prone to interference of shearing force when being cultured in a klinostat and the continual contraction time of the cardiocytes is short. The method comprises the following steps: preparing a mixed solution; preparing cardiocytes; preparing a cell suspension, adding the cell suspension to a divalent positive ion solution to obtain microballoons; culturing the mircoballons in a culture medium to form the three-dimensional histioid cardiac muscular tissue. The three-dimensional histioid cardiac muscular tissue prepared by the method prepared by the invention can be cultured on a long-term basis,needs no passage and can maintain contraction of the cardiac muscular tissue for a long time. The method is applied to the research fields of space biology, aerospace medicine and tissue engineering.

Description

technical field [0001] The invention relates to a method for preparing a three-dimensional myocardium-like tissue. Background technique [0002] The outer space environment has the characteristics of microgravity, ultra-low temperature, high vacuum and strong radiation. Among them, microgravity has a significant impact on human physiological functions. Aerospace medical researchers at home and abroad have conducted a large number of medical observations and studies on the effects of microgravity on human body functions, and found that long-term space flight can cause a certain degree of pathological changes in physiological functions, mainly including cardiovascular system, muscular system, immune system and Pathological changes of the skeletal system. Since the cardiovascular system maintains the normal life activities of the human body, it is of great significance to study the influence of microgravity on the human cardiovascular system, explore its mechanism and formulat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N11/04C12Q1/02
Inventor 田维明李钰郑红霞
Owner HARBIN INST OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com