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Method of loading a crystallization device

A crystallization device and crystallization technology, which is applied in peptide preparation methods, crystallization dishes, separation methods, etc., can solve the problems of easy errors in the weighing process, inappropriate limited quantities, etc., and achieve high flexibility, convenience, and flexibility sexual effect

Active Publication Date: 2011-08-31
QIAGEN GMBH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, for medium- or high-throughput crystallization experiments, it is not appropriate to individually weigh defined amounts of matrix-forming compounds.
Furthermore, the weighing process is also error-prone since the exact ratio of cubic phase and membrane protein must be provided for ideal and reproducible crystallization experiments

Method used

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  • Method of loading a crystallization device

Examples

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Embodiment approach

[0040] According to one embodiment, the crystallization device is a protein crystallization plate. Preferably the plate is in porous form. A suitable crystallization apparatus and suitable embodiments thereof are described in detail above; reference is made above.

[0041] According to one embodiment, the matrix-forming compound capable of forming a membrane protein crystallization matrix has at least one of the following properties:

[0042] a) capable of forming a mesophase; and / or

[0043] b) capable of forming a cubic phase; and / or

[0044] c) capable of forming a sponge phase; and / or

[0045] d) is a lipid; and / or

[0046] e) is amphiphilic; and / or

[0047] f) contains saturated or unsaturated fatty acid chains; and / or

[0048] g) are alcohol derivatives of fatty acids.

[0049] Further details regarding these compounds, suitable mixtures and compositions comprising additives are discussed in detail above in connection with the loading method, and apply equally to ...

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Abstract

The present invention pertains to a method for loading a crystallization device and for manufacturing a crystallization device comprising multiple receptacles with a pre-defined amount of at least one matrix-forming compound capable of forming a crystallization matrix for a membrane protein, said method comprising the following steps: a) modifying the state of aggregation of said at least one matrix-forming compound to a fluidic state which allows dispensing said at least one matrix-forming compound, and b) dispensing a defined amount of said at least one matrix-forming compound into at least one receptacle of the crystallization device, wherein said dispensed matrix-forming compound solidifies within said receptacle. Thereby, prefilled crystallization devices are obtained which can be used as consumables in particular in automated crystallization processes. Also provided are protein crystallization methods using respectively prepared crystallization devices.

Description

field of invention [0001] The present invention relates to the crystallization of biopolymers, in particular membrane proteins, and to an automatable method for loading a crystallization device with a matrix forming compound. technical background [0002] Three-dimensional protein structures are of very high commercial value as they enable rational (structure-based) design and engineering of novel drug molecules that bind proteins of interest. Furthermore, the engineering of novel proteins with desired properties can thereby be facilitated. The three-dimensional structure of proteins and other biopolymers is usually determined by X-ray diffraction of corresponding crystals. In order to observe them by X-ray diffraction crystallography, high-quality crystals of biopolymers must be obtained. Unfortunately, the science of protein crystal growth currently cannot predict the conditions under which a particular protein will crystallize. Therefore, in conjunction with many other...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D9/00C30B7/00C30B29/58B01L3/06
CPCB01L3/06B01D9/00B01D9/0077B01D9/02C07K1/00C30B7/00B01D2009/0086C07K1/306C07K14/705
Inventor J·库比西克F·谢弗J·拉巴恩G·布尔特
Owner QIAGEN GMBH
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