Lachnum melanin hair dye and preparation method thereof
The technology of P. granulosa and melanin is applied in the field of P. granulosa melanin hair dye and its preparation, and can solve the problems of unstable chromophore group of natural dyes, unsatisfactory fastness of natural dyes, poor stability of natural dyes, and the like, To achieve good light protection, glossy dyeing effect, natural color effect
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Embodiment 1
[0011] First, according to the Chinese patent application number 200910145058.4 "A kind of Trichomyces granulosa and its method for preparing melanin by liquid fermentation", the extracellular melanin is prepared by using the Trichomyces granulosa YM-223 with the preservation number of CCTCC No: M 209193: Discobacteria YM-223 was inoculated in a 250mL Erlenmeyer flask filled with 125mL fermentation medium (PDA modified medium), and cultured at 25°C with constant temperature shaking at 200r / min for 10-15 days. After the cultivation, the supernatant was obtained by suction filtration, and the pH value of the obtained supernatant was adjusted to 10-11 with a NaOH aqueous solution with a concentration of 2 mol / L. Adjust the pH value to 2-3, let it stand overnight, centrifuge the resulting flocculent precipitate at 5,000r / min for 10 minutes, pour off the supernatant; add deionized water to the obtained solid precipitate and mix well Centrifuge at 5,000 r / min for 10 min, pour off th...
Embodiment 2
[0024] First, according to the Chinese patent application number 201110072469.2 "A strain of Trichodia singeri and its liquid fermentation method for preparing intracellular melanin", it was prepared by using a strain of Trichosanthes singeri YM-292 with a preservation number of CCTCC No: M 2011054 Intracellular melanin: inoculate Trichodia singerii YM-292 bacteria block into a 250mL Erlenmeyer flask filled with 50mL fermentation medium, and culture at 27°C with constant temperature shaking at 160r / min for 8-10 days. After the cultivation, the fermentation broth was centrifuged to obtain mycelia, and the mycelia was dried at 60° C. and ground, and the weight of mycelia (g): volume of NaOH solution (mL) = 1: 50-100 Liquid ratio Add the mycelium to the NaOH solution with a concentration of 1mol / L, extract it in a water bath at 80°C for 40 minutes, filter it with suction, adjust the pH value of the filtrate to 2-3 with 12mol / L hydrochloric acid, let it stand overnight, and floccul...
Embodiment 3
[0032] First, according to the Chinese patent application number 200910145058.4 "A kind of Trichomonad and its method of preparing melanin by liquid fermentation", the intracellular melanin is prepared by using the preservation number of CCTCC No: M 209193 Trichomonas YM-223: The bacterium block of Discobacteria YM-223 was inoculated in a 500mL Erlenmeyer flask filled with 250mL fermentation medium, and cultured at 26°C with constant temperature shaking at 150r / min for 10-15 days. After the cultivation, the fermentation broth is centrifuged to obtain mycelia. The obtained mycelium was dried at 55° C., ground, and the mycelium was added to a concentration of 2 mol / In L NaOH solution, leaching in 90°C water bath for 5h, suction filtration, the filtrate was adjusted to pH 2-3 with 12mol / L hydrochloric acid, and stood overnight, flocculent precipitates formed. After the flocculent precipitate was centrifuged at 5000r / min for 10min, deionized water was repeatedly added to the sep...
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