Method for raising deguelin content with rare earth element

A technology of rare earth elements and deterin, applied in the biological field, can solve the problems of slow growth of callus of deer vine, easy browning of deterin content, increased cost, etc., to improve growth status, reduce browning degree, The effect of increasing content

Inactive Publication Date: 2011-08-31
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, problems such as slow growth of callus of deervine plant, easy browning (browning rate of 50% to 60%), and low deterin content often appear in the study of degeria plant tissue culture. In large-scale production, The inability to obtain an efficient and stable culture system will undoubtedly increase the cost of production, so it is very important and necessary to establish an efficient and stable plant cell culture system in production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Wash the soil and dust on the surface of deer vine leaves with tap water, rinse 2-3 times with distilled water, absorb the surface moisture with filter paper, soak in 75% alcohol for 30 seconds, rinse 2-3 times with sterile water, use 2% Soak in sodium hypochlorite solution for 6 minutes, then wash 5-8 times with sterile water, blot the water on the surface of the leaves with sterile filter paper, cut the leaves into small pieces of about one square centimeter with a sterilized puncher, and inoculate them on Add 6.5g / L agar, 30g / L sucrose, on the MS plant medium containing 0.5mg / L cytokinin and 2mg / L cytokinin (MS medium is the most common medium currently used. It has a higher The concentration of inorganic salts can ensure the mineral nutrition required for tissue growth and can also accelerate the growth of callus), placed in a 25°C incubator and cultured in the dark for 20 to 30 days, and then subcultured. After 3 subcultures, After 20-30 days of culture each time, ...

Embodiment 2

[0028] The calli obtained by the deervine leaves obtained in Example 1 after being induced and subcultured for 3 times were inoculated into the MS plant cell culture medium containing 0.005mM La, and 6.5g / L agar was added per liter of medium, and 30g / L L sucrose, 6 mg / L cytokinin and 6 mg / L cytokinin, cultivated in 25 ° C for 15 days in a culture division, compared with the control group without rare earth elements, the callus obtained increased by 30%, while brown The conversion rate was reduced by 35%. Transfer the cultured callus to MS plant cell medium containing 0.01mM Ce, add 6.5g / L agar, 30g / L sucrose, 0.5mg / L cytokinin and 4mg / L cell per liter of medium Auxin, cultured in the dark for 35 days in a 25°C incubator, compared with the control group without rare earth elements, callus browning decreased by 27%, and the content of detenin increased by 166%.

Embodiment 3

[0030] The callus obtained by the deervine blade obtained in Example 1 after being induced and subcultured for 3 times was inoculated into a mixture containing 0.02mM mixed rare earth elements (its composition ratio and molar ratio are La 2 o 3 : CeO 2 : Pr 6 o 11 : Sm 2 o 3 =90:120:10:35) MS plant cell culture medium, add 6.5g / L agar, 30g / L sucrose, 3mg / L cytokinin and 3mg / L cytokinin per liter medium, at 30°C After 30 days of culture in the culture division, compared with the control group without adding rare earth elements, the browning of callus was reduced by 38%, and the obtained callus increased by 45%. This callus is transferred on the MS plant cell culture medium that contains 0.05mM Ce, every liter of culture medium adds 6.5g / L agar, 30g / L sucrose, 1mg / L cytokinin and 4mg / L cytokinin, When cultured in a 20°C incubator in the dark for 25 days, compared with the control group without adding rare earth elements, the browning of the callus was reduced by 50%, and t...

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PUM

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Abstract

The invention provides a method for raising deguelin content with rare earth element, comprising the steps of inoculating derris callus to routine plant cell medium containing 0.005 to 0.2mmol / L of rare earth element or rare earth element oxide, adding 6.5g / L of agar, 30 g / L of cane sugar, 0.5 to 6 mg / L of cytokinin, and 1 to 6mg / L of cell auxin for light-shading culture at a temperature of 20 DEG C to 30 DEG C for 15 to 30 days, inoculating derris callus once more to routine plant cell medium containing 0.005 to 0.2mmol / L of rare earth element or rare earth element oxide after the culture is finished, and adding 6.5g / L of agar, 30 g / L of cane sugar, 0.5 to 6 mg / L of cytokinin, and 1 to 6mg / L of cell auxin for light-shading culture at a temperature of 20 DEG C to 30 DEG C for 15 to 50 days. The invention improves the growing situation of derris callus cells, promotes the growth of derris cells, and raises the accumulation amount of deguelin by inoculating derris callus to culture medium containing rare earth elements.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for increasing the content of deguelin by using rare earth elements. Background technique [0002] fish vine ( Derris trifoliata Lour) is the genus of Fabaceae Deervine ( Derris Lour ) plants, there are more than 80 species of this genus in the world, mainly distributed in tropical and subtropical regions, and about 20 species are found in my country, widely distributed in Fujian, Guangdong, Yunnan, Taiwan and other provinces in China. Deerides ( Derris Lour ) leguminous plants such as Demeritus ( Derris trifoliata Lour.), Maoyu vine ( Derris elliptica (Roxb.) Benth.), and Ashleaf ( Tephrosia Pers ) leguminous plant edamame ( Tephrosia vogelii Hook f. ) and so on contain a large amount of rotenone compounds, which are the main source of rotenone compounds. [0003] Deterin is one of the rotenone compounds. Recent studies have shown that deterin ca...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 甘纯玑陈潇彬李坚黄钰洁谢苗
Owner FUJIAN AGRI & FORESTRY UNIV
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