Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bacillus brevis for preventing and treating hot pepper epidemic disease as well as preparation method and application of biological agent

A technology of Bacillus brevius and pepper blight, applied in the direction of microorganism-based methods, biochemical equipment and methods, botany equipment and methods, etc., can solve the problems of no pepper biocontrol bacteria preparations, and achieve the problem of no pesticide residues, The effect of advanced production technology and low cost

Inactive Publication Date: 2012-09-19
PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the biological control of Bacillus brevus is mainly in the stage of isolation and screening and the determination of antibacterial activity in vitro, and there is no biocontrol preparation for pepper production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacillus brevis for preventing and treating hot pepper epidemic disease as well as preparation method and application of biological agent
  • Bacillus brevis for preventing and treating hot pepper epidemic disease as well as preparation method and application of biological agent
  • Bacillus brevis for preventing and treating hot pepper epidemic disease as well as preparation method and application of biological agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Isolation of bacteria

[0036] The plate dilution method was used to separate and separate the bacteria collected from the vegetable field soil in Wushan, Tianhe District, Guangzhou, Guangdong, and the soil samples were diluted to 10 with sterile water. -6 、10 -7 、10 -8 、10 -9 、10 -10 Gradient concentration. NA solid medium (beef extract 3g, yeast extract 1g, peptone 5g, glucose 10g, agar 18g, water to 1000mL, pH 7.2-7.4) was sterilized and poured into 9cm dishes (about 15mL per dish). Use a pipette to pipette 0.1ml of each concentration and put it into each dish, spread it evenly with a coating rod, repeat 10 dishes for each concentration, culture at 30-32°C for 24-30h, and select 10-30 bacteria per dish to grow The plate of the colony was picked, and aseptically suppressed the slant of the NA test tube, cultured at 30-32°C for 24-30h, and then stored for later use.

[0037] (2) Screening of bacteria for preventing and treating pepper blight

[0038] The bac...

Embodiment 2

[0072] Preparation of Bacillus brevis 26-13 biological preparation

[0073] (1) Activation of strains: After preparing NA medium (composition per liter: beef extract 3g, yeast extract 1g, peptone 5g, glucose 10g, water to 1000mL, pH 7.2-7.4), each specification Fill a 500mL Erlenmeyer flask with 100mL of NA medium, and sterilize at 121 for 15min. Inoculate Bacillus brevis 26-13 into sterilized NA medium for shake flask culture; shake flask culture conditions are as follows: 30-32°C, rotation speed 160-200 rpm, culture time 15 hours; the activated Strains;

[0074] (2) The mass reproduction of the thalline is a continuous amplification fermentation process.

[0075] First use a 100L fermenter to ferment, and the fermented liquid is LA medium (composition of every liter: cornstarch 3g, soybean protein powder 15g, corn steep liquor 25g, MnSO 4 0.0308mg, K 2 HPO 4 3g, KH 2 PO 4 1.5g, MgSO 4 0.5g, FeSO 4 0.1g, CaCO 3 1g, water is fixed to 1000mL, pH7.0), and liquid ...

Embodiment 3

[0079] Experiment of Controlling Pepper Blight with Biological Agent of Brevibacillus 26-13

[0080] (1) biological agent: the biological agent obtained in Example 3, the bacterial cell content is about 5 × 10 11 CFU / g.

[0081] (2) Phytophthora capsici zoospore suspension: Inoculate Phytophthora capsici into V8 culture medium (V8 vegetable juice 100mL, CaCO 3 0.2g, dilute to 1000mL in water), culture at 26-28°C for 5-7 days, filter the mycelium to obtain spore suspension, count with a hemocytometer and dilute to a concentration of 800cfu / mL.

[0082](3) Greenhouse test: Cultivate pepper seedlings in a greenhouse (28-30°C) until the pepper seedlings grow to 3 leaves. One day before the test, the pepper seedlings were fully irrigated to keep the substrate moist. With the Bacillus brevis 26-13 biological agent obtained in Example 2 400×, 800×, 1200× spraying and root drenching treatment, with 50% dimethomorph wettable powder 2000 times liquid as the control agent, clear wate...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a Bacillus brevis for preventing and treating a hot pepper epidemic disease as well as a preparation method and application of a biological agent. The Bacillus brevis for preventing and treating the hot pepper epidemic disease is Bacillus brevis 26-13 which was preserved in China center for type culture collection (CCTCC) on January 8, 2011, wherein the preservation number is CCTCC M2011009. The preparation method of the biological agent comprises the following steps: fermenting the Bacillus brevis 26-13 and drying the obtained fermentation liquid; and mixing a fermentation liquid dried substance with a vector and a protective agent so as to obtain the biological agent for preventing and treating the hot pepper epidemic disease. The biological agent is environment-friendly, has the advantages of no pesticide residue, low cost, advanced production process and the like, and the effect of the biological agent which is diluted to 400 times on the hot pepper epidemic disease reaches above 95%.

Description

technical field [0001] The invention relates to biological pesticide control technology, in particular to a preparation method and application of a brevibacillus for preventing and treating pepper blight and a biological agent. Background technique [0002] Capsicum blight is a worldwide soil-borne disease caused by Phytophthora capsici. The rate of diseased plants in fields with mild disease is 15-30%, resulting in 20-25% yield loss. More than 50%, or even no income. In recent years, with the increase of pepper planting area in protected areas, the occurrence of pepper blight has a tendency to increase year by year. At present, there is no ideal disease-resistant variety in production, and the prevention and control of diseases mainly depends on chemical agents. The long-term single use of metalaxyl and other similar agents has significantly improved the drug resistance of Phytophthora capsici, and chemical pesticides are harmful to the ecological environment and human hea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/01
Inventor 林壁润蒲小明沈会芳李培谦潘群英
Owner PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com