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Botanical fungicide composition containing Eupatorium Adenophorum extract as well as preparation method and application of botanical fungicide composition

A botanical fungicide, Eupatorium adenophorum, is applied in the field of pesticides to achieve obvious control effects, environmental friendliness, and good synergistic effect

Inactive Publication Date: 2018-03-23
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, at present, there are no research reports and patents on the use of Eupatorium adenophorum extract and other medicinal plant extracts to combat tobacco black shank.

Method used

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  • Botanical fungicide composition containing Eupatorium Adenophorum extract as well as preparation method and application of botanical fungicide composition
  • Botanical fungicide composition containing Eupatorium Adenophorum extract as well as preparation method and application of botanical fungicide composition
  • Botanical fungicide composition containing Eupatorium Adenophorum extract as well as preparation method and application of botanical fungicide composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Preparation of a botanical fungicide composition containing Eupatorium adenophorum extract

[0031](1) Extraction: collect the non-lignified part of the aboveground part of Eupatorium adenophorum plant, dry it in the shade, crush it, pass it through an 80-mesh sieve, add solvent, extract it ultrasonically at room temperature for 3 times, each time for 30 minutes, combine the filtrate, and pour the filtrate into a rotary evaporator , concentrated by rotary evaporation under reduced pressure and transferred a small amount into a graduated volumetric flask, and fixed the volume to 1 g / mL with solvent to obtain Eupatoria adenophorum extracts Z1-Z3. The parameters are shown in Table 1.

[0032] Weigh the plants used for traditional Chinese medicine, pulverize them, add a solvent, extract them by ultrasonic at room temperature for 3 times, each time for 30 minutes, combine the filtrate, pour the filtrate into a rotary evaporator, concentrate it by rotary evaporation...

Embodiment 2

[0041] (1) Extracting Eupatorium adenophorum extract: collect the non-lignified part of the aboveground part of Eupatorium adenophorum plant and then pulverize it after drying in the shade, pass through a 60 mesh sieve, add the extraction solution, the mixture of the pulverized Eupatoria adenophorum and the extraction solution The ratio of solid to liquid (g / mL) is 1:10, and the extraction solution is ethanol with a volume content of 95%. Ultrasonic extraction at room temperature is carried out 3 times, each time for 20 min. The combined filtrate is poured into a rotary evaporator and decompressed. Concentrate by rotary evaporation until a small amount is transferred to a volumetric flask, and the volume is adjusted to 1g / mL with a solvent to obtain Eupatoria adenophorum extract;

[0042] (2) extract the crude extract of traditional Chinese medicine: take the Chinese medicinal plant, pulverize it, add the extraction solution, the solid-liquid ratio (g / mL) of the Chinese medicin...

Embodiment 3

[0046] (1) Extracting Eupatorium adenophorum extract: collect the non-lignified part of the aboveground part of Eupatorium adenophorum plant and pulverize it after drying in the shade, pass through a 100 mesh sieve, add the extraction solution, the mixture of the pulverized Eupatoria adenophorum and the extraction solution The ratio of solid to liquid (g / mL) is 1:8, the extraction solution is water, ultrasonically extract at room temperature 3 times, each time for 40 minutes, combine the filtrate, pour the filtrate into a rotary evaporator, and concentrate it to a small amount by rotary evaporation under reduced pressure. Put it into a volumetric flask, and dilute it to 1g / mL with a solvent to obtain the extract of Eupatorium adenophorum;

[0047] (2) extract the crude extract of traditional Chinese medicine: take the Chinese medicinal plant, pulverize it, add the extraction solution, the solid-liquid ratio (g / mL) of the pulverized Chinese medicinal plant and the extraction sol...

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Abstract

The invention discloses botanical fungicide composition containing Eupatorium Adenophorum extract as well as a preparation method and an application of the botanical fungicide composition and belongsto the technical field of pesticides. The composition comprises components in percentage by weight as follows: 2%-4% of the Eupatorium Adenophorum extract, 0.2%-0.5% of crude extract of traditional Chinese medicine, 1%-30% of auxiliaries and the balance of water, and the content sum of the components in percentage by weight is 100%. The composition has good synergistic effect in the range of a certain ratio and has obvious control effect on Phytophthora parasitica var.nicotianae; medicinal plants are taken as the raw materials, so that the problem of pesticide residues is solved; compared withsingle use of chemical pesticides, the botanical fungicide composition is environmentally friendly; besides, during using, effective concentration is low, and the cost is low.

Description

technical field [0001] The invention belongs to the technical field of pesticides, and in particular relates to a botanical fungicide composition containing Eupatorium adenophorum extract and its preparation method and application. Background technique [0002] Plant diseases have always been one of the important restrictive factors for high-quality and high-yield crops. Among plant diseases, 70%-80% of the diseases are caused by pathogenic fungus infection. Plant fungal diseases not only directly cause the decline of crop yield and quality, but also some pathogenic fungi can secrete and produce a variety of toxins and metabolites harmful to humans and animals during the process of infecting crops, posing a great threat to the safety of agricultural products. [0003] Tobacco black shank (Phytophthora parasitica var.nicotianae) is one of the most devastating soil-borne fungal diseases in tobacco production. Tobacco is extremely destructive. Tobacco black shank can infect ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/12A01N65/20A01N65/36A01N65/00A01N65/30A01N65/32A01P3/00
CPCA01N65/00A01N65/12A01N65/20A01N65/30A01N65/32A01N65/36
Inventor 李强戴美玲刘晓颖谭军张一扬
Owner HUNAN AGRICULTURAL UNIV
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