Method for identifying plant introgression line with high tissue culture ability
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A tissue culture, infiltration technique, applied in the fields of botanical equipment and methods, plant regeneration, horticultural methods, etc.
Inactive Publication Date: 2013-05-01
CHINA AGRI UNIV
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[0007] The genetic analysis of rice cultivability is limited to the study of cultivated rice, and the study of using wild rice as a material to analyze the genetics of its cultivability has not been reported
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Embodiment 1
[0046] Embodiment 1, screening the Yuanjiang wild rice infiltration line with high tissue culture capacity
[0047] method one:
[0048] 1. Obtainment of Yuanjiang wild rice infiltration line
[0049] Based on the geographical distribution, ecological environment and botanical characteristics of common wild rice in Yuanjiang, Yunnan (O. rufipogon Griff), Yuan Pingrong; He Qingrui; Wen Guosong, Yunnan Agricultural Science and Technology, 4, 3-4, 1994, public Obtained from China Agricultural University.) as the female parent (donor parent), and the excellent indica rice (O. sativa ssp. , the public can obtain from China Agricultural University.) as the male parent (recipient parent), prepare a hybrid combination, use Teqing as the recurrent parent, backcross 3 times in a row, and self-cross 1 time. During the continuous backcrossing process, no option, obtained from different BC 3 f 1 383 BC of a single plant 3 f 2 Strains, continuous selfing 1-2 times to obtain BC 3 f ...
Embodiment 2
[0128] Embodiment 2, identification
[0129] The infiltration lines obtained by method one are identified below.
[0130] 1. Verification of culture-related traits of several infiltration lines
[0131] Isolation and Identification of Typical Indica Rice Varieties Teqing, 9311 (Indica 9311 Radiation Mutants, Hu Biaolin, Xie Jiankun, Bao Jinsong, Yu Shouwu, Zhang Zheng, Molecular Plant Breeding, 2006, 4(2) 181-188, available from China Obtained from Agricultural University.), Guichao 2 (Genotype Analysis of Rice Variety Guichao 2 Heading Stage, Luo Linguang, Wan Jianmin, China Rice Science, 2002 16 (2) 99-105, public available from China Agricultural University obtained) was used as a control to further verify the culture ability of the above 8 infiltration lines. In the tissue culture process, it is the same as the aforementioned method, but pre-differentiation is carried out after two subcultures (pre-differentiation is to improve the state of callus, and pre-differentiatio...
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Abstract
The invention discloses a method for screening a Yuanjiang common wild rice introgression line material with high tissue culture ability. The method comprises the following steps of: 1) performing inducing culture on seeds of plant introgression lines to be identified to obtain calluses, and counting the inductivity; 2) sequentially subculturing the calluses obtained in step 1) twice to obtain first subcultured calluses and second subcultured calluses respectively, and counting the browning rate of the first subcultured calluses and the browning rate of the second subcultured calluses respectively; and 3) differentiating the calluses obtained in step 2), and counting the differentiation rate. The experiment proves that: Yuanjiang common wild rice and Teqing are utilized to construct an introgression line population, mature embryos of 80 introgression lines are cultured, and phenotype data of characters related to culture abilities of the introgression lines are investigated to screen the line with high culture ability by using the Teqing as control, so that a transgenic receptor with high culture ability can be provided for genetic improvement and functional genome research of rice.
Description
technical field [0001] The present invention relates to a method for identifying high tissue culture potency plant infiltration lines. Background technique [0002] Using transgenic technology to introduce exogenous good genes into plants is an important way to improve the efficiency of crop breeding. However, to carry out large-scale transgenic breeding, it is necessary to have a good tissue culture system with high callus proliferation and regeneration efficiency. As a model crop for genome research, rice has a relatively complete genetic transformation technology system, but most indica rice varieties are prone to browning during callus subculture, resulting in reduced differentiation ability. A lot of research, but so far, an effective tissue culture system suitable for different indica rice varieties has not been established, which seriously affects the research and application of indica rice transgenics. [0003] Using different varieties, different explants, and dif...
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