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Biosynthesizing method of cadmium telluride quantum dots

A cadmium telluride quantum dot and biosynthesis technology, applied in fermentation, nanotechnology, etc., can solve the problems of low quantum yield, inconvenient operation, and low quality, and achieve good biocompatibility, uniform shape, and reproducibility Good results

Inactive Publication Date: 2011-07-27
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the cost of aqueous phase synthesis is low, the surface charge and surface properties are highly adjustable, and various functional groups are easy to introduce, but most of them use highly toxic chemical agents (such as trioctylphosphine or trioctylphosphine oxide) and need to react at high temperature
Although there are also reports of biological synthesis of quantum dots, such as the preparation of CdSe quantum dots by yeast, a series of complicated post-processing processes are involved, the operation is inconvenient, and the quality of the obtained is not high, and the quantum yield is low.

Method used

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  • Biosynthesizing method of cadmium telluride quantum dots
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  • Biosynthesizing method of cadmium telluride quantum dots

Examples

Experimental program
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Embodiment 1

[0019] With 10g peptone, 5g yeast extract, 10g.L -1 Prepare Escherichia coli medium (LB medium, pH=7.0) with NaCl, inoculate Escherichia coli K12 (E.coli K12 (ATCC 29181)), and cultivate until the absorbance of the bacteria reaches 0.6 under 600nm light to obtain a seed solution spare.

[0020] Transfer 1ml of Escherichia coli seed solution into a single-neck flask, dilute it into 45ml of LB medium (composition as above), then add 4ml of 0.04mol.l under constant stirring -1 CdCl 2 Solution, 100mg trisodium citrate dihydrate, 1ml 0.04mol.l -1 Na 2 TeO 3 Solution, 60mg mercaptosuccinic acid (MSA), 50mg sodium borohydride (NaBH 4 ), continue to use a rotary oscillator (200rpm) to cultivate at 37°C for 1 to 10 days, centrifuge at 6000 rpm to remove E. coli cells, collect the supernatant and concentrate to obtain biosynthesized protein-coated CdTe quantum dots .

[0021] Four samples with reaction times of 2, 4, 6 and 8 days were tested on a UV-visible spectrometer to obta...

Embodiment 2

[0023] With 10g peptone, 5g yeast extract, 10g.L -1 The culture medium of E. coli (LB medium, pH=7.0) was prepared with NaCl, inoculated with E. coli (E. coli K12 (DH10B)), cultured until the absorbance of the bacteria under 600nm light reached 0.6, and the seed solution was obtained for use.

[0024] Transfer 1ml of Escherichia coli seed liquid into a single-necked flask, dilute it into 45ml of LB medium (composition as above), then add 4ml of 0.05mol.l under constant stirring -1 CdCl 2 Solution, 100mg trisodium citrate dihydrate, 4ml 0.04mol.l -1 Na 2 TeO 3 Solution, 60mg mercaptosuccinic acid (MSA), 50mg sodium borohydride (NaBH 4 ), continue to use a rotary oscillator (200r, p, m) to cultivate at 37°C for 6 days, and centrifuge step by step according to the method of Example 1 to collect CdTe quantum dots with a diameter of about 4.5nm wrapped in proteins.

Embodiment 3

[0026] With 10g peptone, 5g yeast extract, 10g.L -1 The culture medium of E. coli (LB medium, pH=7.0) was prepared with NaCl, inoculated with E. coli (E. coli K12 (MG1655)), cultured until the absorbance of the bacteria under 600nm light reached 0.6, and the seed liquid was obtained for later use.

[0027] Transfer 1ml of Escherichia coli seed liquid into a single-necked flask, dilute it into 45ml of LB medium (composition as above), then add 4ml of 0.04mol.l under constant stirring -1 CdCl 2 Solution, 100mg trisodium citrate dihydrate, 1ml 0.04mol.l -1 Na 2 TeO 3 solution, 60mg L-cysteine, 50mg sodium borohydride (NaBH 4 ), continue to use a rotary oscillator (200r, p, m) to cultivate at 28°C for 5 days, and collect protein-wrapped CdTe quantum dots with a diameter of about 3.5nm by step-by-step centrifugation according to the method in Example 1.

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Abstract

The invention provides a biosynthesizing method of cadmium telluride quantum dots, which comprises the steps of: with alkali metal tellurite as a Te source, water-solubility as a Cd source, escherichia coli cells as a catalyst and sulfhydryl compound as a stabilizing agent, culturing the Te source and the Cd source in a culture system suitable for escherichia coli in an environment of citrosodine and sodium borohydride to obtain CdTe quantum dots, wherein the quantity ratio of substances of the Te source to the Cd source is 1:(1-10). The biosynthesizing method of cadmium telluride quantum dots mainly has the benefits that: in the escherichia coli biosystem, cadmium telluride quantum dots which have adjustable fluorescence wavelength, wide luminous range and good biocompatibility can be biosynthesized by using the tellurite as a raw material and through controlling the culture time; and the method is simple to operate, has good repeatability, and is a high-efficiency way to prepare cadmium telluride quantum dots which have uniform shape, excellent optical performance and good biocompatibility.

Description

(1) Technical field [0001] The invention relates to a biological synthesis method of cadmium telluride quantum dots. (2) Background technology [0002] II-VI semiconductor quantum dots (quantum dots) have unique electronic and optical properties. In the past two decades, quantum dots of different types, shapes, and compositions have been widely studied and practically applied, and a variety of Methods for the synthesis of quantum dots. [0003] At present, there are two main ways to prepare cadmium telluride quantum dots, one is the metal-organic phase reaction at high temperature (see: C.B. Murray, et al., Journal of the American Chemical Society, Vol. 115, 8706 (1993)). The other is an aqueous phase synthesis method in which thiol is used as surface ligand protection (see: Zhang Hao, et al., Advanced Materials, Vol. 15, 1712 (2003)). Among them, the cost of aqueous phase synthesis is low, the surface charge and surface properties are highly adjustable, and various functi...

Claims

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Application Information

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IPC IPC(8): C12P3/00B82Y40/00
Inventor 包海峰吕靖陈灿梁玉洁
Owner HANGZHOU NORMAL UNIVERSITY
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