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Method for diagnosing soil pollution condition by using earthworm biomarkers

A biomarker and soil pollution technology, which is applied in the field of earthworm biomarker diagnosis of soil pollution, achieves the effect of low cost, simple operation and good sensitivity

Inactive Publication Date: 2013-11-06
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still no relevant literature on how to use these index systems related to earthworms to diagnose soil pollution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Weigh 200g of artificial standard soil and test soil respectively. The formula of artificial standard soil is by weight percentage: industrial quartz sand 69% (containing more than 50% of 0.05-0.2 mm fine particles), kaolin 20%, moss soil 10% , CaCO 3 1%, mixed well, and adjusted the soil pH to 6.5 with 1 M NaOH. The artificial standard soil and the test soil were respectively used as the culture medium, and the clean cow dung or pig manure (in line with the bio-organic fertilizer industry standard NY884-2004) was used as the earthworm food, and the soil moisture content was kept at 35% by weight; Healthy adults and 24 hours after bowel cleansing, Eisenia chinensis ( Esisenia fetida ), cultured at 25°C for 5 days.

[0029] The earthworms were put into a petri dish with wet filter paper for 24 hours, and then the content of oxygen free radicals (ROS) in the earthworms was measured. Specifically: after the earthworms were rinsed with ice-cold saline, quickly weighed 0...

Embodiment 2

[0035] Basic steps are the same as in Example 1. Weigh 300g of artificial standard soil and test soil respectively. The formula of artificial standard soil is by weight percentage: industrial quartz sand 69% (containing more than 50% of 0.05-0.2 mm fine particles), kaolin 20%, moss soil 10% , CaCO 3 1%, mixed well, and adjusted the soil pH to 6.5 with 1 M NaOH. The artificial standard soil and the test soil were respectively used as the culture medium, and the clean cow dung or pig manure (in line with the bio-organic fertilizer industry standard NY884-2004) was used as the earthworm food, and the soil moisture content was kept at 30% by weight; Enderia worm ( Eisenia andrei ), cultured at 25°C for 7 days.

[0036] The earthworms were put into a petri dish with wet filter paper for 24 hours to clean the intestines, and then the content of malondialdehyde (MDA) in the earthworms was determined. Specifically: take 200 μL of earthworm tissue homogenate, add 8.1% SDS, 0.2 mL...

Embodiment 3

[0042] Basic steps are the same as in Example 1. Weigh 500g of artificial standard soil and test soil respectively. The formula of artificial standard soil is by weight percentage: industrial quartz sand 69% (containing more than 50% of 0.05-0.2mm fine particles), kaolin 20%, moss soil 10% , CaCO 3 1%, mixed well, and adjusted the soil pH to 6.5 with 1 M NaOH. The artificial standard soil and the test soil were respectively used as the culture medium, and the clean cow dung or pig manure (in line with the bio-organic fertilizer industry standard NY884-2004) was used as the earthworm food, and the soil moisture content was kept at 30% by weight; Healthy adults and 24 hours after bowel cleansing ( Pheretima aspergillum ), cultured at 25°C for 7 days.

[0043] The earthworms were put into a petri dish with wet filter paper for 24 hours, and then the protein carbonyl content (PCO) of the earthworms was measured. Specifically: use 50 mM potassium phosphate (pH 7.4) buffer (co...

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Abstract

The invention provides a method for diagnosing soil pollution condition by using earthworm biomarkers, and belongs to the technical field of soil pollution monitoring and control. In the method, artificially cultured earthworm is used as a standard test animal; four oxidative damage test indexes, namely oxygen free radical, malondialdehyde content, protein carboxyl content and reductive glutathione-oxidative glutathione proportion, are used as the biomarkers for diagnosing soil pollution; clean artificial soil is used as standard soil; and the pollution condition of the tested soil can be quickly and sensitively diagnosed by analyzing the difference of the four oxidative damage indexes in vivo of the earthworm after the earthworm is cultured in the clean artificial soil and the tested soil for certain time. The method can be used for comprehensively diagnosing the soil pollution condition and the health degree, and the method has good sensitivity for trace pollutants in the soil and can be used as a monitoring method for soil pollution early warning. Compared with a chemical analysis method, the method has the characteristics of simple operation and low cost.

Description

technical field [0001] The invention relates to a method for soil pollution monitoring, in particular to a method for diagnosing soil pollution status with earthworm biomarkers, that is, using oxidative damage indicators in earthworms to monitor soil pollution status, including heavy metal pollution and organic matter pollution. Background technique [0002] In recent years, with the rapid development of industrial and agricultural production, a large number of pollutants have entered the soil environment and coexisted through various channels, and the soil pollution that my country is facing is showing an increasing trend. Soil is an important part of the environment, and it is also the final carrier of various pollutants. It bears about 90% of the pollutants in the environment from various sources. When foreign pollutants exceed the self-purification ability of the soil, it will destroy the normal state of the soil. function, causing it to lose its natural ecological balan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/24G01N24/10G01N21/17G01N21/33G01N21/64
Inventor 王晓蓉谢显传薛银刚顾雪元耿金菊
Owner NANJING UNIV
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