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Method for producing L-ascorbyl palmitate through non-aqueous phase enzymatic synthesis

A technology of ascorbyl palmitate and enzymatic synthesis, applied in directions such as fermentation, can solve the problems of unfavorable environment and low yield of waste liquid discharge, and achieve the effects of facilitating large-scale production, high yield and improving yield

Inactive Publication Date: 2011-07-20
GUANGZHOU FOOD IND RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The chemical synthesis method with mature technology is still the most important method for the industrial production of L-ascorbyl palmitate. The enzyme-catalyzed synthesis of L-ascorbyl palmitate has not yet been industrialized, and there are technically low yields and waste liquid discharges that cause environmental problems. unfavorable

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0032] Add 10Kg of L-ascorbic acid, 30Kg of palmitic acid, and 350L of tert-butanol into the reaction kettle, add immobilized lipase Novozyme435, and catalyze the reaction for 40 hours at a temperature of 55°C and a stirring speed of 120rpm. The obtained reaction liquid is recovered by suction filtration to recover the immobilized lipase and undissolved L-ascorbic acid, and the resulting filtrate was evaporated under reduced pressure at 55°C to remove the solvent tert-butanol. The residue was dissolved in 400L ethyl acetate, washed twice with deionized water, the ethyl acetate phase was separated, and the ethyl acetate solvent was evaporated under reduced pressure at 50°C to obtain a crude product. The crude product was dissolved by heating with 400L toluene at 60°C, cooled and crystallized at room temperature, centrifuged to obtain a filter cake, washed twice with n-hexane, centrifuged at 50°C, and vacuum-dried at -0.09MPa for 2 hours to obtain the target product L-ascorbyl pa...

example 2

[0034]Add 10Kg of L-ascorbic acid, 24Kg of palmitic acid, and 380L of tert-butanol into the reaction kettle, add immobilized lipase Novozyme435, and catalyze the reaction at a temperature of 50°C and a stirring speed of 120rpm for 36 hours. The obtained reaction solution is suction filtered to remove the immobilized lipase and undissolved L-ascorbic acid, the resulting filtrate was evaporated under reduced pressure at 55°C to remove the solvent tert-butanol, the residue was dissolved in 350L ethyl acetate, washed twice with deionized water, the ethyl acetate phase was separated, and then reduced to 50°C The solvent ethyl acetate was removed by evaporation under reduced pressure to give crude product. The crude product was dissolved by heating with 350L toluene at 60°C, cooled and crystallized at room temperature, centrifuged to obtain a filter cake, washed twice with n-hexane, centrifuged at 50°C, and vacuum-dried at -0.09MPa for 2 hours to obtain the target product L-ascorbyl ...

example 3

[0036] Add 10Kg of L-ascorbic acid, 32Kg of palmitic acid, and 350L of tert-butanol into the reaction kettle, add the immobilized lipase Lipozyme TLIM, and catalyze the reaction at a temperature of 55°C and a stirring speed of 120rpm for 40 hours. The obtained reaction solution is suction filtered to remove the immobilized fat Enzyme and undissolved L-ascorbic acid, the resulting filtrate was evaporated under reduced pressure at 58°C to remove the solvent tert-butanol, the residue was dissolved in 300L ethyl acetate, washed twice with deionized water, the ethyl acetate phase was separated, and then heated at 50°C The solvent ethyl acetate was evaporated under reduced pressure to obtain a crude product. The crude product was dissolved by heating with 200L toluene at 60°C, cooled and crystallized at room temperature, centrifuged to obtain a filter cake, washed twice with n-hexane, centrifuged at 52°C, and vacuum-dried at -0.09MPa for 2 hours to obtain the target product L-ascorby...

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Abstract

The invention discloses a method for producing L-ascorbyl palmitate through non-aqueous phase enzymatic synthesis. The method comprises the following steps of: (1) mixing L-ascorbic acid with palmitic acid in a non-aqueous medium, adding immobilized lipase for catalytic reaction for 20-50 hours; (2) filtering the reaction liquid obtained in the step (1) to remove the immobilized lipase and undissolved L-ascorbic acid and evaporating the obtained filtrate under reduced pressure to remove a solvent; and (3) dissolving the residue obtained in the step (2) with ethyl acetate, washing with deionized water, separating an ethyl acetate phase out, evaporating under reduced pressure to remove the solvent, dissolving the residue with toluene, recrystallizing, centrifuging, washing the obtained substance with normal hexane and drying the obtained undissolved substance in vacuum to obtain an L-ascorbyl palmitate product. The method relates to non-aqueous enzymatic synthesis, has the advantages of high catalytic efficiency, mild reaction condition, low pollution, simple process, readily-available raw materials, high yield and convenience for large-scale production, and is accordant with the practical situation of the food additive production industry in China.

Description

technical field [0001] The invention relates to a production method for enzymatically synthesizing L-ascorbyl palmitate. Background technique [0002] Antioxidants are substances that prevent or delay food oxidation, improve food stability and prolong storage life. It can prevent or delay the oxidation of oil and fat-soluble components (such as vitamins, carotenoids, etc.) Thereby improving the stability of food and prolonging the shelf life of food. At present, there are many types and varieties of food antioxidants at home and abroad, and their application range in food is becoming more and more extensive. However, the use of some highly toxic products is being restricted or phased out. Now, food antioxidants are developing in the direction of non-toxic or low-toxicity and high efficiency. [0003] L-Ascorbyl Palmitate (Ascorbyl Palmitate, English abbreviation AP) is a white or slightly yellow powder crystal with a slight citrus odor, extremely insoluble in water, and ea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/04
Inventor 曾永青晏日安李善军
Owner GUANGZHOU FOOD IND RES INST
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