Brevibacillus brevis TW effervescent granule and preparation method thereof

Abstract

The invention relates to a brevibacillus brevis TW effervescent granule. The brevibacillus brevis TW effervescent granule is prepared through the following steps of: mixing a brevibacillus brevis TW strain single colony plaque PDA (Potato Dextrose Agar) liquid culture solution with soybean meal, corn meal, starches, cane sugar, yeast powder, calcium carbonate and MnSO4 by adding water; mixing bacterial fermentation liquor obtained by fermenting under the conditions of 100% dissolved oxygen, the pH of 7.0-7.2 and the temperature of 30 DEG C with the cane sugar, polyvinyl alcohol, citric acid monohydrate, potassium tartrate and sodium bicarbonate according to a proportion; screening, granulating, and drying through steam flows at a temperature of 60 DEG C, wherein the total concentration ofviable bacteria of a finished product is about 1*10<9>-1*10<10> cfu / g. The brevibacillus brevis TW effervescent granule can be effectively suspended on a water surface and then fast disintegrated, releases the biocontrol bacteria of living bodies and enables the biocontrol bacteria to attach to the contact surface of the stem of a rice plant and the water surface, thereby effectively cutting off or spatially occupying the initial invasion of rice rhizoctonia solani on the rice stem and achieving the purpose that control effect on rice sheath blight reaches more than 80 percent; and the brevibacillus brevis TW effervescent granule is convenient for application, has low cost and can be preserved for 2-3 years at normal temperature.

Description

technical field [0001] The invention relates to a small Bacillus pumilus TW effervescent granule preparation and a preparation method for preventing and treating rice sheath blight. Background technique [0002] Rice sheath blight (Rice sheathblight) caused by the plant pathogenic fungus Rhizoctonia solani (Rhizoctonia solani) is currently the main disease of rice in my country. The disease can cause 5% to 45% yield loss all year round, and 70% yield reduction in high-incidence years, or even no harvest. At present, the control of rice sheath blight is mainly based on the agricultural antibiotic Jinggangmycin, but Jinggangmycin has been widely used since the 1970s, and the drug resistance has increased, and the dosage has gradually increased. Once the sheath blight breaks out, it is difficult to use Jinggangmycin. Control the damage of sheath blight in the short term. Especially at present, farmers mostly use the method of low-water fine spraying, and the drug-receiving pa...

AI Technical Summary

Problems solved by technology

At present, the control of rice sheath blight is mainly based on the agricultural antibiotic Jinggangmycin, but Jinggangmycin has been widely used since the 1970s, and the drug resistance has increased, and the dosage has gradually increased. Once the sheath blight breaks out, it is difficult to use Jinggangmycin. Controlling the damage of sheath blight in a short period of time

Especially at present, farmers use the method of low-water fine spraying, and the drug-receiving parts are mainly the leaves and necks of rice, while the disease of rice is mostly in the stem of the plant, so it cannot reach the diseased part, so that the control of the disease Unsatisfactory effect

In recent years, some biocontrol bacterial and fungal preparations have been developed for the control of rice diseases, but the existing fungal preparations are difficult to preserve, and the validity period is too short; and the efficacy of the medicine is difficult to reach the stem of rice plants, and the control effect is not ideal

Images