Method for detecting drift treatment of target gene
A detection method and gene technology, applied in the field of gene drift processing and detection, can solve the problems that it is difficult to avoid the participation of exogenous gene drift, and achieve the effect of avoiding drift and ensuring environmental safety
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[0042] 1 Scope
[0043] Including human β-defensin-3 gene transgenic cattle and sheep cell culture fluid, nuclear transfer operation fluid, reconstituted embryo culture fluid, and all liquids in the operation process of cell culture and nuclear transfer of offspring of transgenic β-defensin-3 cattle and sheep Qualitative PCR detection of the treatment and drift of the target gene.
[0044] It is suitable for the detection of gene drift in the production process of transgenic cows and goats derived from human defensin-3 gene.
[0045] 2 Terms, definitions and acronyms
[0046] 2.1 Target gene
[0047] The hBD3 gene is located in the p22-23 region of human chromosome 8, including 2 exons and 1 intron, with a total length of 1156bp.
[0048] 2.2 polymerase chain reaction polymerase chain reaction (PCR)
[0049] The template gene sequence is first denatured at high temperature to become a single strand. Under the action of DNA polymerase and suitable reaction conditions, the t...
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