Device and method for biological culture of cell or tissue engineering

Abstract

The invention relates to a bioreactor and method, particularly a reactor and method for seed cell culture or cell / biological material compound culture in cytobiology, tissue engineering and medicine. The method for biological culture of cell or tissue engineering comprises the following steps: putting biological culture raw materials of cell or tissue engineering into a culture vessel with a U-shaped bottom surface, W-shaped bottom surface or V-shaped bottom surface; and driving the culture vessel to annularly swing by using an annular swing power system, wherein the culture vessel annularly swings by using the center as the pivot, and each point on the same circumference sequentially swings up and down. The invention also discloses a device for implementing the method. Under the action of the power provided by the external annular swing, the invention can reduce the cell damage and provide a uniform culture environment under ideal mass transfer conditions.

Description

technical field [0001] The invention relates to a bioreactor and a method, in particular to a reactor and a method for performing seed cell culture or compound culture of cells and biological materials in cell biology, tissue engineering and medicine. Background technique [0002] Tissue engineering is a science that applies the principles and methods of engineering and life sciences to construct biological substitutes to restore, maintain or enhance the function of damaged organs or tissues. The construction of this biological substitute requires biological materials, seed cells and a suitable culture environment, and the bioreactor that can link the three together. [0003] In the field of tissue engineering, most of the seed cells used to construct tissue engineered tissues or organs are anchorage-dependent cells, and the source of seed cells is often primary cultured cells. From the perspective of safety, these cells should not be genetically modified. Transformation an...

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Problems solved by technology

However, the wave bioreactor uses the up and down reciprocating motion provided by the shaking table to enhance the mass transfer. This method has insufficient lateral mass transfer and the cells at both ends may collide with the walls of the culture bag during large wave movements, resulting in Cell damage; while the torrent bioreactor uses the horizontal rotation movement provided by the shaker to strengthen the horizontal mass transfer, but the culture medium at the bottom is still horizontally rotating, and there are still defects of poor vertical mass transfer; it is especially important Unfortunately, these two bioreactors can only be used for the expansion of ordinary animal cells. There is no case for tissue engineering seed cells, nor can they be used for the construction of tissue engineering cell scaffold complexes, especially when an air-liquid interface is required. Construction of cultured tissue-engineered skin

Neither of them can provide ideal gas-liquid two-phase culture environment or culture conditions of gas-liquid interface

[0009] The current culture of tissue engineering constructs is mostly carried out manually in the laboratory, and the cell scaffold complex is cultured in a petri dish. This static culture method has obvious poor mass transfer inside the tissue engineering constructs. Low oxygen content inside the construct, combining with stirred bioreactors or using perfusion bioreactors can solve some of the mass transfer problems, but does not improve the oxygen content inside the culture much

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