Full-automatic gel-preparation electrophoresis ultraviolet glue sweeping and cutting instrument

Abstract

The invention discloses a full-automatic glue-preparation electrophoresis ultraviolet glue sweeping and cutting instrument which comprises a box body. The box body is divided into three layers, a baffle is arranged in the box body, the baffle divides the interior of the box body into six cells on the left side and the right side, the upper part of the box body is provided with a panel, and a liquid crystal screen, a numerical control panel and a control circuit respectively connected with the liquid crystal screen and the numerical control panel are arranged on the panel of the box body respectively; the left side and the right side of the second layer of the box body are respectively provided with a periscope view port; and in the six cells, an elevator, a drying device, an automatic cleaning device, an air-exhaust liquid-exhaust device, a mechanical arm, a plastic gel supporting plate, an adjustable glue preparation tank, a stirring rod, microcontrol integrated automatic glue preparation equipment, a miniature sample adding gun, an ethidium bromide container, a liquid adding device, a periscope, an electrophoresis tank, an ultraviolet glue sweeping camera, a glue cutting instrument, a centrifuge tube clamp and an ethidium bromide dye vat are comprised. The full-automatic glue-preparation electrophoresis ultraviolet glue sweeping and cutting instrument disclosed in the invention has the advantages of automation, integration, convenience and quickness, and greatly increases the efficiency, the precision and the safety of experiments.

Description

technical field [0001] The invention relates to an experimental instrument, in particular to a fully automatic gel electrophoresis ultraviolet sweeping gel cutting instrument. Background technique [0002] In molecular biology experiments, in order to identify and extract target gene fragments, preparation of agarose gel, electrophoresis, ultraviolet scanning and photographing, and gel cutting and recovery are all indispensable steps. In the current traditional method, these four steps are completed manually. In this process, due to the manual contact with high-risk drugs such as ethidium bromide, the experimenters will wear gloves during the experiment, but due to the multi-step process of the experiment The experimenter must put on and take off the gloves many times, and always pay attention to avoid the gloves from polluting other instruments in the laboratory. However, since the glue is exposed to the air during the experiment, due to the volatility of ethidium bromide i...

AI Technical Summary

Problems solved by technology

In the current traditional method, these four steps are completed manually. In this process, due to the manual contact with high-risk drugs such as ethidium bromide, the experimenters will wear gloves during the experiment, but due to the multi-step process of the experiment The experimenter must put on and take off the gloves many times, and always pay attention to avoid the gloves from polluting other instruments in the laboratory. However, since the glue is exposed to the air during the experiment, due to the volatility of ethidium bromide itself, it will be very To a certain extent, it causes air pollution in the laboratory, especially for the experimenters who are conducting this experiment, ethidium bromide will enter the human body through the respiratory tract, causing great harm to the human body

Due to improper protection, there have been many reports of the health of the experimenters being damaged during the experiment. In addition, because the four steps are all completed by manual operations, especially in the process of cutting glue, the UV sweeping lasts for a long time. It has a destructive effect on the recovery band. On the other hand, manual operation will waste a lot of time, and due to the cascade amplification of manual operation errors, the accuracy of the experimental results cannot be guaranteed.

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