Energy-saving method for culturing haliotis diversicolor aquatilis
A nine-hole abalone, energy-saving technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, climate change adaptation, etc. Residue and other problems, to achieve the effect of improving immunity, improving intestinal environment, and accelerating growth rate
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[0030] (1) Preparation of the Bdellovibrio Bruldoid Bacteria Liquid for Haliotis diversicolor aquatilis Seedling Cultivation
[0031] Inoculate 0.5mL of 10 in a conical flask containing 100mL of nutrient broth liquid medium (peptone 10g, beef extract powder 3g, sodium chloride 5g, pH 7.4±0.2) 7cfu / mL Aeromonas hydrophila (Aeromonas hydrophila, strain number: GIM 1.172, sourced from the Guangdong Provincial Microbial Culture Collection Center), 200rpm, 35°C shaker culture for 12 hours, the culture solution was centrifuged at 4°C, 5000rpm for 15min , discard the supernatant. Suspend and precipitate the bacterial cells with 5mL normal saline, and then add them to a liquid culture medium containing 100mL DNB (nutrient broth 0.8g, casein acid hydrolyzate 0.5g, yeast extract 0.1g, dissolve in 1000mL distilled water, pH 7.2 ~ 7.6) Erlenmeyer flask, add 1mL containing 10 3 Bdellovibrio BDFM05 in pfu / mL (CCTCC M209172). Incubate on a constant temperature shaker at 250 rpm at 28°C fo...
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