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Rubber tree powdery mildew in vitro culture method and culture medium thereof

A powdery mildew bacteria, in vitro culture technology, applied in the field of plant pathology, can solve the problems of large occupied area and difficult to meet the test requirements, and achieve the effect of small occupied space, simple method and convenient operation

Inactive Publication Date: 2011-04-06
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the storage method of bagged seedlings has the disadvantages of large area and less young leaves that can be used, so it is difficult to meet the test requirements.

Method used

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Experimental program
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Embodiment Construction

[0019] The present invention will be described in further detail below in conjunction with specific embodiments.

[0020] 1. Preparation of isolated leaves

[0021] A, select the tender leaves of the rubber tree in the discoloration period;

[0022] B, after the fresh rubber tree leaf of gathering is washed three times with sterile water, soak 5 minutes in 0.5% sodium hypochlorite solution, then rinse three times with sterile water, drain;

[0023] 2. Strain selection: select fresh rubber tree powdery mildew asexual conidia on rubber tree diseased leaves;

[0024] 3. Nutrient solution and culture plate preparation

[0025] A. Use the following formula to prepare nutrient solution: calcium chloride 100~200mg / L, 6-benzylaminopurine 5~10mg / L, salicylic acid 10~80mg / L, vitamin C 0.2~0.8mg / L, thiosulfuric acid Sodium 0.2~0.8g / L, activated carbon 1~5g / L, 0.2 times MS medium macroelements (ammonium nitrate 3.3g / L, potassium nitrate 3.8g / L, calcium chloride dihydrate 0.88g / L, sulfu...

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Abstract

The invention discloses a rubber tree powdery mildew in vitro culture method and a culture medium thereof, belonging to the technical field of plant pathology. The rubber tree powdery mildew in vitro culture method comprises the following steps of: firstly, preparing a nutrient solution and a culture disc; secondly, preparing an in vitro leaf; and thirdly, inserting the in vitro leaf after treating into a PCR plate hole filled with the nutrient solution, lightly brushing powdery mildew on an infected rubber tree leaf on the in vitro leaf which is treated with the nutrient solution by using a writing brush or a brush which is sterilized in 75 percent alcohol, arranging in an artificial climate box with the temperature of 23 DEG C and the moisture of 90 percent, carrying out light culture for 16h per day and carrying out dark culture for 8h per day. The rubber tree powdery mildew in vitro culture method has the advantages of convenience, durability and high efficiency and can be used for culturing rubber tree powdery mildew in a large scale, thereby realizing in vitro culture of the rubber tree powdery mildew on the leaf and solving the problems of culture and preservation of the rubber tree powdery mildew.

Description

technical field [0001] The invention belongs to the technical field of plant pathology, and in particular relates to a method for in vitro culture of rubber tree powdery mildew bacteria and a culture medium thereof. Background technique [0002] Rubber tree powdery mildew is one of the most important leaf diseases of Hevea brasiliensis caused by fungus infection of the genus oidium heveae B.A. Steinm. Since it was first discovered in Java, Indonesia in 1918, it has spread all over the world's rubber planting areas. When the disease is serious, the powdery mildew causes the leaves and flowers of the rubber tree to fall, which reduces latex production, weakens the vigor of the rubber tree, and dies new shoots, which has a great impact on the growth and output of the rubber tree. [0003] Rubber tree powdery mildew, as a climate disease, mainly breaks out in cold and humid spring conditions, and rubber tree powdery mildew is an obligate parasite, which cannot grow on artificial...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 涂敏蔡海滨华玉伟黄华孙
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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