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New extraction method of agaricus bisporus polysaccharide

A technology for extracting Agaricus bisporus and polysaccharides, which is applied in the field of new technology for deep processing of agricultural and sideline products, can solve the problems of low yield, difficulty in large-scale production, and high price, and achieve the effects of low protein content, simple process operation, and easy-to-obtain equipment

Inactive Publication Date: 2011-04-06
宜昌益农科技开发有限公司
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

[0003] At present, the extraction process of Agaricus bisporus polysaccharide is very complicated and the yield is low, the production cost is high, and it is difficult to put it into large-scale production, resulting in high market price, which is unacceptable for ordinary patients.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0040] (1) Rinse: Select freshly picked Agaricus bisporus with thick flesh, no disease spots, and no deformities, cut off the stem, pour it into the rinsing tank and rinse for 1 minute to remove the soil and impurities on the mushroom.

[0041] (2) Cooking: Pour Agaricus bisporus into a stainless steel jacketed pot filled with boiling water at 95°C and blanch for 2 minutes to make it transparent and not rotten, and set aside for later use.

[0042] (3) Grinding: take 2kg Agaricus bisporus, add purified water in a ratio of 1:2 (W / W), and grind it in a food grinder to obtain mushroom paste.

[0043] (4) Refining: The mushroom mud can be obtained by passing the mushroom mud through a colloid mill.

[0044] (5) Homogenization: pass the mushroom mud through a high-pressure homogenizer to break the cells of Agaricus bisporus to obtain a homogeneous mixture of Agaricus bisporus.

[0045] (6) Enzyme reaction and leaching: add 0.2% (W / W) cellulase to the homogeneous mixture of Agaricu...

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PUM

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Abstract

The invention provides an extraction method of agaricus bisporus polysaccharides, comprising the following steps of: carrying out pretreatments, i.e. rinsing, cooking, grinding, pulp refining, homogenizing, and the like, and then carrying out enzyme reaction and extracting; then removing proteins; precipitating the agaricus bisporus polysaccharides under the action of ethanol; and freeze-drying to obtain the agaricus bisporus polysaccharides. The method has simplified process and low cost and is easy for industrialized production.

Description

technical field [0001] The invention relates to a new technology for deep processing of agricultural by-products, in particular to a new method for extracting Agaricus bisporus polysaccharide. Background technique [0002] Agaricus bisporus polysaccharides (ABPS) is an immune-modulating anti-tumor auxiliary drug. It is a high-molecular biological product extracted from the fruiting body of Agaricus bisporus by using natural product chemical technology. Its molecular weight is generally 40- 800 000. Modern medical research shows that Agaricus bisporus polysaccharide (ABPS) has resistance to human virus infection and carcinogenesis. Agaricus bisporus polysaccharide can not only inhibit the growth of S-180 solid tumor in mice, but also interfere with the proliferation of SMMC-7721 human liver cancer cells cultured in explants, and has good anti-tumor activity. It has a significant effect on the treatment of persistent hepatitis, chronic hepatitis, hepatomegaly, and early live...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 黄艺邵伟
Owner 宜昌益农科技开发有限公司
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