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Process for extraction separation of bamboo leaf flavonoids by two aqueous phases

A technology of bamboo leaf flavonoids and two-phase water, which is applied to medical preparations containing active ingredients, plant/algae/fungus/moss components, plant raw materials, etc., can solve the problems of cumbersome operation, time-consuming, and low extraction rate, and achieve The effect of rapid phase separation, shortening time and ensuring stability

Inactive Publication Date: 2011-03-30
HUNAN PROVINCE HONGJIANG HUAGUANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of these methods are that the operation is complex, time-consuming, the extraction rate is low, and the quality of the extracted bamboo leaf flavonoids is not high, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Add 150ml of ethanol with a mass ratio of 70% to the bamboo leaves that are impurity-removed, cleaned, dried, and pulverized, and refluxed at 90°C for 2 times, each time for 2 hours, filtered at 100 mesh, and the combined filtrates, concentrated filtrates at -0.09MPa, Ethanol is recovered to obtain a concentrated solution.

[0015] (2) Place the concentrated solution in a centrifuge, centrifuge at a speed of 2000r / min for 5min, and collect the supernatant for later use.

[0016] (3) According to the supernatant, weigh polyethylene glycol with a mass ratio of 11% and 8% dextran, mix uniformly, add the supernatant, adjust pH7-9, mix uniformly, centrifuge (2000r / min, 5min) to separate the phases and collect the dextran phase (lower phase);

[0017] (4) Use the dextran phase of the step c to routinely separate dextran to obtain bamboo leaf flavonoids.

Embodiment 2

[0019] (1) Add 140ml of ethanol with a mass ratio of 70% to the bamboo leaves that have been impurity-removed, cleaned, dried, and pulverized, and refluxed at 90°C for 2 times, each time for 2 hours, filtered through 100 mesh, and the combined filtrates, -0.08MPa concentrated filtrates, Ethanol is recovered to obtain a concentrated solution.

[0020] (2) Place the concentrated solution in a centrifuge, centrifuge at 2000r / min for 5min, and collect the supernatant for later use.

[0021] (3) According to the supernatant, weigh 10% polyethylene glycol and 8% dextran by mass, mix uniformly, add the supernatant, adjust pH7-9, mix uniformly, centrifuge (2000r / min, 5min) to separate the phases and collect the dextran phase (lower phase);

[0022] (4) Use the dextran phase of the step c to routinely separate dextran to obtain bamboo leaf flavonoids.

Embodiment 3

[0024] (1) Add 160ml of ethanol with a mass ratio of 70% to the bamboo leaves that have been impurity-removed, cleaned, dried, and pulverized, and extracted twice at 90°C under reflux, each time for 2 hours, filtered through 100 mesh, combined filtrates, and concentrated filtrates at -0.1MPa, Ethanol is recovered to obtain a concentrated solution.

[0025] (2) Place the concentrated solution in a centrifuge, centrifuge at a speed of 2000r / min for 5min, and collect the supernatant for later use.

[0026] (3) According to the supernatant, weigh polyethylene glycol with a mass ratio of 12% and dextran of 8%, mix uniformly, join in the supernatant, adjust pH7-9, mix uniformly, centrifuge (2000r / min, 5min) to separate the phases and collect the dextran phase (lower phase);

[0027] (4) Use the dextran phase of the step c to routinely separate dextran to obtain bamboo leaf flavonoids.

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Abstract

The invention discloses a process for the extraction separation of bamboo leaf flavonoids by two aqueous phases. For overcoming the drawbacks of complex operation, time consumption, low extraction rate and low quality of extracted bamboo leaf flavonoids and the like of the conventional method for extracting bamboo leaf flavonoids from bamboo leaves, the processes comprises: adding ethanol into bamboo leaf powder, filtering, concentrating filtrate, recovering ethanol, and obtaining concentrated solution; centrifuging the concentrated solution and collecting supernate; adding polyethylene glycol and glucan into the supernate, uniformly mixing, centrifuging and collecting a glucan phase; and separating glucan by a conventional method and obtaining bamboo leaf flavonoids. The method is used for extracting bamboo leaf flavonoids from the bamboo leaves can amplify a project and realize continuous operation and is simple, high in extraction rate, large in handling capacity, low in energy consumption and production cost, free from arousing inactivation or denaturation of bamboo leaf flavonoids, short in phase separation time, small in interface tension and free from organic solvent residue.

Description

technical field [0001] The invention relates to a process for extracting bamboo leaf flavonoids from bamboo leaves, in particular to a process for separating bamboo leaf flavonoids by two-phase extraction. Background technique [0002] The main active ingredient in bamboo leaves is bamboo leaf flavonoids. Bamboo leaf flavonoids have anti-oxidant, anti-bacterial and anti-viral effects, and are a promising anti-oxidant with heat-clearing and detoxifying effects. [0003] Bamboo leaf flavonoids mainly exist in the form of carbon sugar groups. Existing methods for extracting bamboo leaf flavonoids from bamboo leaves generally use water, ethanol aqueous solution and other polar solvents for extraction, or use macroporous adsorption resin for extraction. The disadvantages of these methods are that the operation is cumbersome, time-consuming, the extraction rate is low, and the quality of the extracted bamboo leaf flavonoids is not high. Contents of the invention [0004] The ...

Claims

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Application Information

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IPC IPC(8): C07G99/00A61K36/899
Inventor 黄洪慧
Owner HUNAN PROVINCE HONGJIANG HUAGUANG BIOTECH
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