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Preparation method of chickpea total saponin

A technology of chickpea and total saponins, which is applied in the field of preparation of total chickpea saponins, can solve the problems of complex operation, inapplicability to industrial production, and high cost

Active Publication Date: 2011-03-30
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Methods such as adsorption chromatography, partition chromatography, high performance liquid chromatography, and droplet countercurrent chromatography (DCCC) can purify high-purity saponins and single compounds, but their operations are complicated and expensive, and they are not suitable for industrial production.

Method used

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  • Preparation method of chickpea total saponin
  • Preparation method of chickpea total saponin
  • Preparation method of chickpea total saponin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] a. Crush the chickpeas, pass through a 40-mesh sieve, take 500g of chickpea flour, add 6# solvent oil, and use a water bath to heat and reflux to degrease. The heating temperature is 40°C. 5 times the amount of 30% ethanol was extracted once by heating and refluxing with an electric heating mantle for 1 hour, the extract was collected, and the ethanol was recovered under reduced pressure;

[0053] b. Concentrate the extract from step a to a mass concentration of 0.87g crude drug / mL, and then purify the concentrated solution with HPD100 macroporous resin. The diameter-to-height ratio of the resin bed is 1:4, the sample loading time is 1h, and the flow rate of the effluent is controlled. 3BV / h, let it stand for 2h, let it fully absorb, wash the resin bed with 1BV of pure water, the flow rate of water washing is 1BV / h, remove the part and impurities that are not adsorbed by the resin, elute with 80% ethanol with a concentration of 3BV, and the flow rate is 1BV / h;

[0054]...

Embodiment 2

[0056] a. Crush the chickpeas, pass through a 50-mesh sieve, take 500g of chickpea flour, add 6# solvent oil, and use a water bath to heat and reflux to degrease. The heating temperature is 55°C. 10 times the amount of 50% ethanol was heated and refluxed for 3 times with an electric heating mantle for 2-3 hours, the extract was collected, and the ethanol was recovered under reduced pressure;

[0057] b. Concentrate the extract from step a to a mass concentration of 0.87g crude drug / mL, and then purify the concentrated solution with HPD300 macroporous resin. The diameter-to-height ratio of the resin bed is 1:4, the sample loading time is 3 hours, and the flow rate of the effluent is controlled. 1BV / h, let it stand for 2h, let it fully absorb, wash the resin bed with 3BV of pure water, the flow rate of water washing is 3BV / h, remove the part that is not adsorbed by the resin and impurities, elute with 1BV of 50% ethanol, the flow rate is 3BV / h;

[0058] c. Collect the ethanol e...

Embodiment 3

[0060] a. Crush the chickpeas, pass through a 40-mesh sieve, take 500g of chickpea flour, add 6# solvent oil, and use a water bath to heat and reflux to degrease at a heating temperature of 70°C. 15 times the amount of 80% ethanol was heated and refluxed with an electric heating mantle to extract 4 times, the time was 3 hours, the extraction temperature was 95°C, the extract was collected, and the ethanol was recovered under reduced pressure;

[0061] b. Concentrate the extract from step a to a mass concentration of 0.87g crude drug / mL, and then purify the concentrated solution with SA-3 macroporous resin. The bed diameter-to-height ratio of the loaded resin is 1:4, and the sample loading time is 6 hours to control the outflow The liquid flow rate is 4BV / h, let it stand for 2h, let it fully absorb, wash the resin bed with 3BV of pure water, the water washing flow rate is 4BV / h, remove the part that is not adsorbed by the resin and impurities, and elute with 4BV of 80% ethanol, ...

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Abstract

The invention relates to a preparation method of chickpea total saponin, comprising the following steps of: crushing and sieving chickpeas; adding solvent oil; degreasing by heating and refluxing; extracting by heating and refluxing; collecting an extracting solution; reducing pressure to recover ethanol; concentrating; purifying a concentrated solution by using macroporous resin; drying in vacuum; and obtaining a purifying product of the chickpea total saponin, wherein the mass content of the purifying product reaches 22-25 percent. Through an in-vitro hypoglycemic experiment, the obtained chickpea total saponin product has an inhibit function on protein-tyrosine-phosphatase 1B, and a result indicates that the chickpea total saponin has better activity; meanwhile, the preparation method has good reproduction quality, and resin can be repeatedly used.

Description

Technical field: [0001] The invention relates to a preparation method of chickpea total saponins. Background technique: [0002] Chickpea (Cicer arietinum L.) originated in West Asia and the Mediterranean coast, and the global planting area has reached 150 million mu. It is currently the second largest edible bean crop in the world. With a growth history of 10 years, it is a commonly used medicinal material in Uyghur medicine. It has been recorded in the Uyghur medicine sub-volume of "Drug Standards" and "Uyghur Medicine Chronicles" of the Ministry of Health of the People's Republic of China. For thin body, low libido, loss of appetite, skin itching and diabetes and other diseases. [0003] Chickpeas are rich in saponins, and studies such as P.A.Ireland have shown that chickpeas contain 0.23-6% of total saponins. The saponins in chickpea are mainly triterpenoid soybean saponins. Saponins have a variety of pharmacological effects, such as anti-cancer, cardiovascular diseas...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61P3/10
Inventor 阿吉艾克拜尔・艾萨程珍吕俏莹阿布力米提・伊力窦君
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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