Unilateral ureteral ligation recanalisation animal model and building method thereof

Abstract

The invention discloses a unilateral ureteral ligation recanalisation animal model and a building method thereof. The method comprises the following steps of: 1) preparing an elastic plastic tube; and 2) after implanting an elastic plastic fat pad into the left ureter of an SD male mouse to pressurize to perform ligation obstruction, performing a surgery 7 days later for the second time, in the surgery, taking out the elastic plastic tube, dredging the left ureter to release the left kidney obstruction and separate the envelope of the left kidney at the same time, and ligaturing the right ureter with a NO. 4 thread. In addition, the invention also discloses the animal model built by the method. The animal model can reduce renal functions and cause renal tubule mesenchyme fibrosis change. The model building period is short; the response is quick; the cost is low; the model is convenient to use; the performance of the model is stable; the repeatability is good; pathomorphological changes of kidney after obstruction can be observed; the functional level of the kidney can be studied; and the relation between the pathomorphological changes and the functional level can be embodied; and the animal model provides a new study target for clinical research and development of medicaments for preventing and treating renal fibrosis.

Description

technical field [0001] The invention belongs to the field of animal models, and in particular relates to a novel animal model of tubulointerstitial fibrosis induced by unilateral ureteral elastic plastic ligation and recanalization in rats. In addition, the invention also relates to a method for establishing the animal model. Background technique [0002] Renal fibrosis is the common pathway and the main pathological basis for the development of renal diseases of various causes to end-stage renal disease (ESRD). This is a serious disease that endangers people's health. Although the prevalence of ESRD in my country (300 / million people) is lower than that in Japan (1642 / million people), Taiwan (1423 / million people) and the United States (1403 / million people), the incidence of chronic kidney disease in my country Quite high, the potential threat of end-stage renal disease is great, and experts predict that the ESRD prevalence rate will increase at a rate of about 10% per year. ...

AI Technical Summary

Problems solved by technology

However, the traditional unilateral ureteral occlusion (UUO) model can only observe the histomorphological changes of renal tissue after obstruction, and it is difficult to evaluate the function of the damaged kidney. Therefore, it is greatly affected in the development of animal models for the prevention and treatment of renal fibrosis drugs. limitations

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