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Method for identifying moisture resistance of sesame during germination

A technology of germination and moisture resistance, which is applied in the field of identification of moisture resistance of sesame germination, to achieve the effect of accelerating the identification process, good repeatability, and less equipment investment

Inactive Publication Date: 2011-03-30
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the method of evaluating the moisture resistance of sesame in the germination stage.

Method used

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  • Method for identifying moisture resistance of sesame during germination
  • Method for identifying moisture resistance of sesame during germination
  • Method for identifying moisture resistance of sesame during germination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Determination of suitable time for flooding treatment of sesame germination stage and main evaluation indicators

[0047] The materials used in the experiment included 7 materials including Zhongzhi 11, Zhongzhi 13, Ezhi 2, Yuzhi 10, H98, ZZM1138 and ZZM1327 and 31 randomly selected sesame materials.

[0048] I. Analysis of the response characteristics of sesame germination to flooding treatment

[0049] The specific method is:

[0050] (1) Select 600 plump seeds of Zhongzhi 11, Zhongzhi 13, Ezhi 2, Yuzhi 10, H98, ZZM1138 and ZZM1327, weigh them, and place them in a petri dish covered with double-layer moist filter paper. Germinate at a constant temperature of 28°C until the seeds are white;

[0051] (2) Select 50 seeds that have germinated to Lubai. The standard of Lubai is: the radicle exposed outside the seed coat is 1 / 2 to 3 / 4 of the seed length, and placed in a 10ml centrifuge tube filled with water, and then After the centrifuge tube was placed upsid...

Embodiment 2

[0071] Example 2: Identification of moisture resistance of 222 sesame germplasm resources in germination stage

[0072] (1) Sample preparation: 222 sesame germplasm resource materials with rich genetic base were selected, and 600 plump seeds were taken for each sesame material;

[0073] (2) Seed germination to dew white: put the seeds of each material evenly in the petri dish that has been pre-laid with double-layer moist filter paper, and cultivate them in the incubator at a constant temperature of 28°C until most of the seeds are dew white. The dew white standard is dew white. The radicle outside the seed coat is 1 / 2 to 3 / 4 of the seed length;

[0074] (3) Flooding treatment: select 2 portions of Lubai seeds with the same number of grains for each material, 50 grains each; place 1 portion in a 10ml centrifuge tube filled with water for sealing, and then place the centrifuge tube upside down in the incubator 28°C constant temperature flooding treatment for 9 hours; the other...

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Abstract

The invention relates to a method for identifying moisture resistance of sesame during germination, which comprises the following steps of: (1) germinating the sesame seed until sprout emerges; (2) soaking treatment; soaking one part of seed; cultivating the other part of seed at constant temperature of 28-30 DEG C, wherein the cultivating time is equal to soaking time, and the cultivated seed is used for non-soaking comparison; (3) aftertreatment: washing the emerged seed subjected to soaking treatment in the step (2) with distilled water and then cultivating the seed at constant temperature of 28-30 DEG C; keeping on cultivating the emerged seed subjected to non-soaking comparison treatment, wherein the cultivating time is equal to the cultivating time of the seed subjected to soaking treatment; (4) measuring and calculating relative survival rate of normal seedling; and (5) dividing moisture resistance level into high moisture resistance, moisture resistance, middle moisture resistance, available moisture resistance and unavailable moisture resistance according to the relative survival rate of the normal seedling. The method is easy to operate, low in cost and reliable in result, has the characteristic of exactly reflecting the reaction to moisture damage during the germination of the sesame, and provides technical and material support for cultivating moisture-resistant variety and identifying moisture resistance of new variety.

Description

technical field [0001] The invention relates to a method for identifying the humidity resistance of sesame in the germination stage. Background technique [0002] Sesame is a crop sensitive to moisture damage, and its moisture tolerance evaluation is of great significance for identifying and screening sesame germplasm resources with strong moisture tolerance, improving and cultivating moisture-tolerant varieties, and promoting stable and increased yield of sesame. of general concern. It has been reported that the evaluation of moisture tolerance of sesame is mostly based on the phenotypic evaluation of moisture damage in the field full-flowering stage (Feng Xiangyun et al., 1991; Zhang Xiurong, 1992; Sun Jian et al., 2008), but this identification method is affected by soil type, air temperature, humidity , wind speed, sunshine and rain, and the growth and development of sesame are healthy or weak. The evaluation period is long, the operation is complicated, and the results...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G7/00A01C1/00
Inventor 张秀荣王林海张艳欣危文亮张晓燕
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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