Method for identifying moisture resistance of sesame during germination
A technology of germination and moisture resistance, which is applied in the field of identification of moisture resistance of sesame germination, to achieve the effect of accelerating the identification process, good repeatability, and less equipment investment
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Embodiment 1
[0046] Example 1: Determination of suitable time for flooding treatment of sesame germination stage and main evaluation indicators
[0047] The materials used in the experiment included 7 materials including Zhongzhi 11, Zhongzhi 13, Ezhi 2, Yuzhi 10, H98, ZZM1138 and ZZM1327 and 31 randomly selected sesame materials.
[0048] I. Analysis of the response characteristics of sesame germination to flooding treatment
[0049] The specific method is:
[0050] (1) Select 600 plump seeds of Zhongzhi 11, Zhongzhi 13, Ezhi 2, Yuzhi 10, H98, ZZM1138 and ZZM1327, weigh them, and place them in a petri dish covered with double-layer moist filter paper. Germinate at a constant temperature of 28°C until the seeds are white;
[0051] (2) Select 50 seeds that have germinated to Lubai. The standard of Lubai is: the radicle exposed outside the seed coat is 1 / 2 to 3 / 4 of the seed length, and placed in a 10ml centrifuge tube filled with water, and then After the centrifuge tube was placed upsid...
Embodiment 2
[0071] Example 2: Identification of moisture resistance of 222 sesame germplasm resources in germination stage
[0072] (1) Sample preparation: 222 sesame germplasm resource materials with rich genetic base were selected, and 600 plump seeds were taken for each sesame material;
[0073] (2) Seed germination to dew white: put the seeds of each material evenly in the petri dish that has been pre-laid with double-layer moist filter paper, and cultivate them in the incubator at a constant temperature of 28°C until most of the seeds are dew white. The dew white standard is dew white. The radicle outside the seed coat is 1 / 2 to 3 / 4 of the seed length;
[0074] (3) Flooding treatment: select 2 portions of Lubai seeds with the same number of grains for each material, 50 grains each; place 1 portion in a 10ml centrifuge tube filled with water for sealing, and then place the centrifuge tube upside down in the incubator 28°C constant temperature flooding treatment for 9 hours; the other...
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