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Method for extracting collagen from fish skin

A collagen and non-collagen technology, applied in fermentation and other directions, can solve the problems of low collagen extraction rate, long extraction time, fat residue, etc., and achieve the effects of good water solubility, less pigment residue, and low fishy smell

Inactive Publication Date: 2011-02-23
JIANGXI SCI & TECH NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the methods for extracting collagen in fish skin mainly include hot water extraction, acid extraction, enzymatic extraction, and methods such as acid extraction and then enzymatic hydrolysis, but most of the above methods have low collagen extraction rate, Or the process is more complicated, the extraction time is longer, the fishy smell is heavier, and the fat residue is serious, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] 1. Fish skin pretreatment: Thaw the frozen fish skin, wash it, and cut it into pieces for later use;

[0009] 2. Degreasing: Add 3 times the weight of 10% isopropanol to the fish skin, extract at 4°C, remove the isopropanol after standing for stratification, repeat 3 times, wash the fish skin with water, and evaporate the isopropanol;

[0010] 3. Remove non-collagen: add 10 times the volume of 5% sodium chloride to the defatted fish skin, stir at 4°C for 24 hours, filter with nylon gauze, add 10 times the volume of distilled water to wash, filter, repeat 3 times;

[0011] 4. Collagen extraction: add 15 times the volume of 0.6 mol / L acetic acid to the obtained fish skin, and at the same time add 5000 U / g pepsin according to the weight of the fish skin, stir for 7 hours, and inactivate the enzyme at 95°C. Centrifuge, concentrate under reduced pressure, and freeze-dry to obtain fish skin collagen powder.

Embodiment 2

[0013] 1. Fish skin pretreatment: Thaw the frozen fish skin, wash it, and cut it into pieces for later use;

[0014] 2. Degreasing: Add 4 times the weight of 10% isopropyl alcohol to the fish skin, extract at 4°C, remove the isopropyl alcohol after standing for stratification, repeat 3 times, wash the fish skin with water, and dry the isopropyl alcohol;

[0015] 3. Remove non-collagen: add 10 times the volume of 5% sodium chloride to the defatted fish skin, stir at 4°C for 16 hours, filter with nylon gauze, add 10 times the volume of distilled water to wash, filter, repeat 3 times;

[0016] 4. Collagen extraction: add 10 times the volume of 0.5mol / L acetic acid to the obtained fish skin, and at the same time add 4000 U / g pepsin according to the weight of the fish skin, stir for 7 hours, and inactivate the enzyme at 95°C. Centrifuge, concentrate under reduced pressure, and freeze-dry to obtain fish skin collagen powder.

Embodiment 3

[0018] 1. Fish skin pretreatment: Thaw the frozen fish skin, wash it, and cut it into pieces for later use;

[0019] 2. Degreasing: Add 5 times the weight of 10% isopropyl alcohol to the fish skin, extract at 4°C, remove the isopropyl alcohol after standing for stratification, repeat 3 times, wash the fish skin with water, and dry the isopropyl alcohol;

[0020] 3. Remove non-collagen: add 10 times the volume of 5% sodium chloride to the defatted fish skin, stir at 4°C for 48 hours, filter with nylon gauze, add 10 times the volume of distilled water to wash, filter, repeat 3 times;

[0021] 4. Collagen extraction: add 20 times the volume of 0.4 mol / L acetic acid to the obtained fish skin, add 3000 U / g pepsin according to the weight of the fish skin, stir for 7 hours, and adjust the pH to 7 to inactivate the enzyme. Centrifuge, concentrate under reduced pressure, and freeze-dry to obtain fish skin collagen powder.

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Abstract

The invention discloses a method for extracting collagen from fish skin. The method comprises the following steps of: treating raw materials; degreasing the treated raw materials with isopropanol; removing non-collagen protein with sodium chloride; leaching with acetic acid; adding acid protease for enzymolysis; centrifugally concentrating; and drying so as to obtain fish skin collagen. The collagen is extracted by performing acid extraction and enzymolysis simultaneously, so that extraction rate is high and extraction time is shortened. The fish skin is treated repeatedly with the isopropanol and fat and pigments are removed fully, so that product quality is enhanced. The method of the invention has high collagen extraction rate and bright color, and does not have fat residues.

Description

technical field [0001] The present invention relates to a method for extracting collagen from fish skin. Background technique [0002] my country is a big fishery country. With the development of the domestic aquatic product processing industry, the leftovers such as fish heads and fish skins generated during the processing of aquatic products are increasing year by year. These leftovers are mainly used to produce low-value feed, fertilizer, etc., or Abandoning it directly is a huge waste. Among them, fish skin is rich in collagen, which can be made into health care products, cosmetics and medical materials, etc., and has a high development prospect. [0003] At present, the methods of extracting collagen from fish skin mainly include hot water extraction, acid extraction, enzymatic extraction, and acid extraction followed by enzymatic hydrolysis. However, most of the above methods have low collagen extraction rates. Or the process is more complicated, the extraction time i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 赵利陈丽丽刘华
Owner JIANGXI SCI & TECH NORMAL UNIV
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