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Method for producing erythritol by using broken rice

A technology of erythritol and broken rice, which is applied in the field of enzyme engineering and microbial fermentation, can solve the problems of complex erythritol separation process, many by-products, and long production cycle, so as to avoid the use of a large number of elution solvents, The effect of high utilization rate of strains and reduction of isolation cost

Inactive Publication Date: 2012-04-18
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on erythritol in our country is relatively late, and the currently used strains include Torula, Torulopsis, and Candida, etc., but the yield is mostly low, there are many by-products, and the production technology is relatively backward. The separation process of erythritol in liquid is complicated, and the purity of erythritol crystals is low, resulting in high production cost of erythritol
In terms of carbon source utilization, cornstarch fermentation is mainly used to produce erythritol in industrial production, which has the disadvantages of high cost, low conversion rate, long production cycle, darker color of fermentation liquid, and complicated separation.
Japanese scholars have studied the production method of using glycerol and alkanes as fermentation carbon sources to ferment and produce erythritol, but it has not yet been industrialized

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Production of Broken Rice Syrup

[0031] Wash the broken rice by-product of indica rice, pick out the impurities in the broken rice, add 40°C water to soak for 2 hours, then use a refiner to refine the pulp, the fineness is 40 mesh, add water to adjust the thick pulp to a concentration of 20°Bé, and make Get broken rice and rice milk;

[0032] Add liquid high-temperature-resistant α-amylase to the broken rice slurry, and add 160 mL of high-temperature-resistant α-amylase per ton of broken rice. Use a high-temperature jet liquefier for liquefaction, the temperature of the first jet is 105°C, and the pressure is maintained for 15 minutes. The temperature of the second injection is 130°C, and the pressure is maintained for 2 minutes. After cooling down to 100°C, pump it into the laminar flow tank, add the same high-temperature-resistant α-amylase to react again, add 240mL of high-temperature-resistant α-amylase per ton of broken rice, the reaction time is 90min, ...

Embodiment 2

[0046] (1) Production of Broken Rice Syrup

[0047] Wash the broken rice by-product of japonica rice, pick out the impurities in the broken rice, add 40°C water to soak for 6 hours, then use a refiner to refine the pulp to a fineness of 40 mesh, add water to adjust the thick pulp to a concentration of 18°Bé, and make Get broken rice and rice milk;

[0048] Add liquid high-temperature-resistant α-amylase to the broken rice slurry, and add 160 mL of high-temperature-resistant α-amylase per ton of broken rice. Use a high-temperature jet liquefier for liquefaction, the temperature of the first jet is 105°C, and the pressure is maintained for 15 minutes. The temperature of the second injection is 130°C, and the pressure is maintained for 2 minutes. After cooling down to 100°C, pump it into the laminar flow tank, add the same high-temperature-resistant α-amylase to react again, add 240mL of high-temperature-resistant α-amylase per ton of broken rice, the reaction time is 100mi...

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Abstract

The invention relates to a method for producing erythritol by using broken rice. In the method, moniliellaacetoabutans (ATCC18455) is used as a production strain and broken rice sugar liquid is used as a fermentation substrate for preparing the high-purity erythritol; and the method comprises three steps, namely preparation of the broken rice sugar liquid, preparation of erythritol fermentation liquid and refining of an erythritol product. The erythritol has high transformation rate, short fermentation period and stable fermentation performance, and has no byproducts such as glycerol and the like; the method reduces the production cost, and has the advantages of low operating cost, small equipment space, high product yield, few impurities and simple process; and the purity of the final product reaches over 98 percent.

Description

technical field [0001] The invention belongs to the technical field of enzyme engineering and microbial fermentation, and in particular relates to the use of P. Moniliella acetoabutans ATCC 18455) as the starting strain, using the enzymatically hydrolyzed broken rice sugar solution as the fermentation substrate to ferment and produce erythritol. Background technique [0002] Erythritol is a four-carbon sugar alcohol with a sweetness of about 70% of sucrose. Its chemical name is 1,2,3,4-butanetetraol, and its molecular formula is C 4 h 10 o 4 . Erythritol is widely distributed in nature and exists in mushrooms, wine, soy sauce, cheese, tissue fluids and body fluids of humans and animals. Due to the unique metabolism of erythritol in the human body, erythritol has many excellent physiological functions, such as zero calorie value, high tolerance, small side effects, suitable for diabetics, and non-cariogenic properties , scavenging free radical function, etc. Therefore, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/18C12P19/14C12R1/645
Inventor 王泽南刘鹏李莹张秋子吴红引苏娅
Owner HEFEI UNIV OF TECH
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