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Rape bee-pollen extract and use thereof

A technology for rape bee pollen and extract, which is applied in the application, food science, drug combination and other directions, and achieves the effects of low requirements for equipment conditions, simple process flow and simple method

Active Publication Date: 2011-02-23
陕西康泰莱生物医药工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about the related effects of rapeseed bee pollen

Method used

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  • Rape bee-pollen extract and use thereof
  • Rape bee-pollen extract and use thereof
  • Rape bee-pollen extract and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A preparation method of rapeseed bee pollen extract, the preparation method comprises the following steps in sequence:

[0020] Weigh 100g of rapeseed bee pollen, add 300ml of distilled water, extract with ultrasonic assistance at 50°C for 1 hour, centrifuge to obtain the extract, repeat the extraction once for the precipitated part, centrifuge, combine the supernatant, concentrate to 1g pollen / ml by rotary evaporation at 50°C, and place in a magnetic field Slowly add 95% ethanol to the concentrated water extract under stirring until no precipitation occurs, let it stand, centrifuge to separate the supernatant, remove the ethanol by rotary evaporation at 50°C and concentrate it into an extract, add methanol until it is completely dissolved, and slowly add Acetone until no more precipitation occurs, centrifuge to take the supernatant, and evaporate to dryness at 50°C. That is, the extract is obtained.

Embodiment 2

[0022] A preparation method of rapeseed bee pollen extract, the preparation method comprises the following steps in sequence:

[0023] Vacuum-dry fresh bee pollen to a moisture content of 10.0%, take 300g of dried pollen and put it into a 1L extractor, and use carbon dioxide with a purity of 99% to boost the pressure of the extraction vessel to a pressure of 45MPa at the temperature of the extraction vessel at 45°C; After 10 minutes of pressure, open the exhaust valve of the extraction tank to quickly empty the carbon dioxide and collect the pollen. Electron microscopy showed that the pollen cells had been broken.

[0024] Weigh 100g of the broken rapeseed bee pollen, add 1000ml of distilled water, extract with ultrasound at 20°C for 1 hour, centrifuge to obtain the extract, repeat the extraction of the precipitated part, centrifuge, combine the supernatant, and concentrate to 0.5g by rotary evaporation at 60°C Pollen / ml, slowly add 95% ethanol to the concentrated solution un...

Embodiment 3

[0026] A preparation method of rapeseed bee pollen extract, the preparation method comprises the following steps in sequence:

[0027] Weigh 100 g of the broken wall rape bee pollen of Example 2, add 500 ml of distilled water, extract with ultrasonic assistance at 50°C for 1 hour, centrifuge to obtain the extract, repeat the extraction of the precipitated part once, centrifuge, combine the supernatant, and rotary evaporate at 60°C to 0.8g of pollen / ml, under magnetic stirring, slowly add absolute ethanol to the concentrated water extract until no precipitation occurs, let stand, centrifuge to separate the supernatant, concentrate under reduced pressure at 60°C to form an extract, add methanol until it is completely dissolved, Slowly add acetone until no more precipitate occurs, remove the precipitate, and concentrate the supernatant to dryness under reduced pressure to obtain the extract.

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PUM

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Abstract

The invention provides an anti-inflammatory, antalgic and antibacterial extract which is extracted from rape bee-pollen according to lots of process researches. Strict tests prove that the extract has anti-inflammatory, antalgic and antibacterial functions and inhibition effect on human leukocyte elastase, excellent antalgic effect on visceral pain caused by ethylic acid and external pain caused by a hot plate in mice and excellent inhibition effect on Escherichia coli, staphylococcus aureus, pseudomonas aeruginosa, bacillus subtilis and other bacterial. The extract can be used in the development of plant-source anti-inflammatory agents, antalgics and bacteriostatic agents. In addition, China's rape pollen productivity is huge, so the rape pollen can be used as the raw material for the plant-source anti-inflammatory agents, antalgics and bacteriostatic agents; and the extract has the advantages of low cost and simple process flow.

Description

technical field [0001] The invention relates to a biological extract, in particular to a rapeseed bee pollen extract and its application. Background technique [0002] Rapeseed (Brassica campestris L.) is a plant of the genus Brassica in the family Brassicaceae. Rapeseed bee pollen is the male reproductive cell of rapeseed collected by bees. It not only contains many nutrients, but also contains many medicinal ingredients related to life sciences. It is a traditional Pure natural nutritional health products. Because of its rich nutrients and numerous physiologically active components, rape bee pollen has a variety of nutritional and pharmacological effects. So far, it has been confirmed that rape bee pollen has the functions of promoting growth and development, anti-fatigue, improving exercise ability, promoting hematopoietic function, regulating immune function, anti-aging, regulating metabolism and endocrine function in the body, inhibiting prostatic hyperplasia and anti-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/64A23L1/076A61P29/00A61P31/04A61K36/31A23L1/29A61K133/00A23L21/20A23L33/00A61K35/644
CPCY02A50/30
Inventor 孙丽萍徐响安仲姚董捷李春艳
Owner 陕西康泰莱生物医药工程有限公司
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