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Method for preparing germinated brown rice by repeated cold stress forcing method

A technology of germinated brown rice and adversity stress, which is applied in the field of preparation of germinated brown rice and germinated brown rice by repeated low-temperature adversity stress method, can solve undisclosed problems in the field of food processing, etc., and achieve the effect of improving germination rate

Inactive Publication Date: 2011-02-09
孙兆远 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Although low temperature stress has the ability to increase the content of soluble sugars and free amino acids in plants, they are all used in botanical fields such as improving plant seed frost resistance and germination rate, and have not disclosed the technology applied in the field of food processing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Preparation of nutrient solution The nutrient solution is made of 0.2mmol / L Ca(NO 3 ) 2 , 0.1mmol / L CaSO 4 , 0.1mmol / L K 2 HPO 4 , 0.3mmol / L K 2 SO 4 , 0.1mmol / L MgSO 4 , 8μmol / L Fe-EDTA, 14μmol / L H 3 BO 3 , 1μmol / L MnSO 4 , 0.1μmol / L CuSO 4 , 0.03μmol / L ZnSO 2 , 0.01μmol / L Na 2 MoO 4 Dilute solution composed of water and nutrient solution to adjust the pH value to 6.0;

[0028] (2) Low temperature stress Soak the husked rice seeds in a nutrient solution with a temperature of 5°C for 10 hours, and inject air into it at the same time;

[0029] (3) High-temperature accelerating germination Place the soaked seeds in a constant temperature accelerating germination room with a temperature of 30° C. and a relative humidity of 85% for 10 hours in the dark;

[0030] (4) Repeat low temperature stress Repeat steps (2), (3) five times;

[0031] (5) Ultra-low temperature stress Soak the germinated rice seeds in a nutrient solution at 0°C for 5 hours;

[0032] (6...

Embodiment 2

[0034] (1) Preparation of nutrient solution The nutrient solution is made of 0.5mmol / L Ca(NO 3 ) 2 , 0.05mmol / L CaSO 4 , 0.15mmol / L K 2 HPO 4 , 0.1mmol / L K 2 SO 4 , 0.3mmol / L MgSO 4 , 14μmol / L Fe-EDTA, 14μmol / L H 3 BO 3 , 3μmol / L MnSO 4 , 0.3μmol / L CuSO 4 , 0.09μmol / LZnSO 2 , 0.03μmol / L Na 2 MoO 4 Dilute solution composed of water and nutrient solution to adjust the pH value to 6.4;

[0035] (2) Low temperature stress Soak the husked rice seeds in a nutrient solution with a temperature of 10°C for 5 hours, and inject air into it at the same time;

[0036] (3) High-temperature accelerating germination Place the soaked seeds at a temperature of 40° C. and a relative humidity of 95% in a constant temperature accelerating germination chamber in the dark for 5 hours;

[0037] (4) Repeat low temperature stress Repeat steps (2), (3) three times;

[0038] (5) Ultra-low temperature stress Soak the germinated rice seeds in a 5°C nutrient solution for 20 hours;

[0039] (6)...

Embodiment 3

[0041] (1) Preparation of nutrient solution The nutrient solution is made of 0.35mmol / L Ca(NO 3 ) 2 , 0.07mmol / L CaSO 4 , 0.13mmol / L K 2 HPO 4 , 0.2mmol / L K 2 SO 4 , 0.2mmol / L MgSO 4 , 10μmol / L Fe-EDTA, 10μmol / L H 3 BO 3 , 2μmol / L MnSO 4 , 0.2μmol / L CuSO 4 , 0.06μmol / LZnSO 2 , 0.02μmol / L Na 2 MoO 4 A dilute solution composed of water and water, and the nutrient solution adjusts the pH value to 6.2;

[0042] (2) Low temperature stress Soak the husked rice seeds in a nutrient solution with a temperature of 7°C for 7 hours, and inject air into it at the same time;

[0043] (3) High-temperature accelerating germination Place the soaked seeds at a temperature of 35° C. and a relative humidity of 90% in a constant-temperature accelerating germination chamber in the dark for 7 hours;

[0044] (4) Repeat low temperature stress Repeat steps (2), (3) four times;

[0045] (5) Ultra-low temperature stress Soak the germinated rice seeds in a nutrient solution at 2°C for 14 h...

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Abstract

The invention provides a method for preparing germinated brown rice by the repeated cold stress forcing method. The method comprises the following steps: placing the brown rice in low temperature solution to force cell tissues of the brown rice to accumulate reactive oxygen; and promoting content of soluble saccharide, free amino acid and polyphenol material to increase. In addition, to prevent excessive reactive oxygen from doing harm to cells, the repeated and short-time low temperature forcing and high temperature stratification are alternatively employed to cause the reactive oxygen to begenerated slowly; as a result, not only high soluble saccharide, free amino acid and polyphenol material are obtained, but also germination percentage of the brown rice is improved; and finally, lower temperature is employed to force the cell membrane of the brown rice to be damaged, so that the soluble sugar and the free amino are extravasated and the new product of the germinated brown rice with good mouthfeel can be produced.

Description

(1) Technical field [0001] The invention relates to a method for preparing germinated brown rice, in particular to a method for preparing germinated brown rice by repeated low-temperature adversity stress method. The invention belongs to the technical field of deep processing of rice. (2) Background technology [0002] Germinated brown rice is a product composed of young sprouts and endosperm with a bran layer after germinating brown rice to a suitable bud length. Currently, research on germinated brown rice is as follows: [0003] Disclosed in 200810047494.3 is a kind of technology that contains cellulase, phytase nutrient solution soaking (temperature 10-40 ℃) to produce germinated brown rice, and its advantage is that the brown rice cortex cellulose is decomposed by the hydrolysis of cellulase, thereby Improve the mouthfeel of brown rice, but the hydrolysis of cellulase is very slow, and the effect of improving the mouthfeel is not obvious. [0004] Disclosed in 200310...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/185A23L7/20
Inventor 孙兆远侯会绒郝涤非
Owner 孙兆远
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