Microbiological feed additive for replacing antibiotics and preparation method thereof
A technology for microbial feed and antibiotic substitution, applied in animal feed, animal feed, additional food elements, etc., can solve the problems of many anti-nutritional factors in feed raw materials, low quality of livestock and poultry products, low digestion and utilization, etc., to activate the growth potential of animals , Enhance the body's anti-stress ability, the effect of low digestion and utilization
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[0090]The preparation method of wort juice: use 2 times the water and 0.5 times the malt of the required wort dose: mash at 55-63 ° C for 4-6 hours and filter it with four layers of gauze to form the wort filtrate (use 0.02 mol / L iodine When the liquid is measured to be yellow to colorless, the saccharification is complete), the sugar concentration in the saccharification liquid is detected with a Baume hydrometer, the filtrate is diluted with water to 10-15 Paulines, and the pH is adjusted to 6.4.
[0091] Prepare Aspergillus niger and Aspergillus oryzae female species: the components of the female species medium and their weight ratios consist of: potato juice: glucose: corn steep liquor=100: 2: 1, and the nutrient solution was sterilized at 120° C. for 20 minutes, cooled to After 35°C, inoculate the strain at a weight ratio of 1 to 3%, and cultivate at a constant temperature of 30-35°C for 36-72 hours;
[0092] The preparation method of potato juice: peel the potatoes, remo...
Embodiment 1
[0100] According to the above method, a microbial feed additive for substituting antibiotics is prepared, and its components and weight percentages are as follows: 25% of Bacillus subtilis, 25% of Saccharomyces cerevisiae, 25% of Aspergillus niger and 25% of Aspergillus oryzae.
Embodiment 2
[0151] According to the above method, a microbial feed additive for substituting antibiotics is prepared, and its components and weight percentages are as follows: 40% of Bacillus subtilis, 40% of Saccharomyces cerevisiae, 10% of Aspergillus niger, and 10% of Aspergillus oryzae.
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