Method for breeding raphanus sativus L. CMS (genic male sterility) lines by using marker assisted selection

Abstract

The invention relates to a method for breeding raphanus sativus L. CMS (genic male sterility) lines by using marker assisted selection, which belongs to the technical field of crop breeding and is used for efficient breeding of excellent male sterility lines in raphanus sativus L. hybrid advantage utilization. The invention adopts the technical scheme that: the method comprises the following steps of: screening inbred lines with excellent traits and stable fertility; selecting specific primers NAUmsF4 and NAUmsR4 to perform PCR amplification on the screened inbred lines, and determining that the inbred lines capable of amplifying 1069bp specific strips cannot be used as candidate maintenance lines; performing restored gene functional marker analysis on the inbred lines incapable of amplifying the specific strips, and screening the inbred lines capable of serving as the candidate maintenance lines; and performing cross and back cross of 4 to 5 generations on the original sterile lines and the identified candidate maintenance lines to obtain new sterile lines and corresponding maintenance lines with excellent traits. The method for quickly screening the candidate maintenance lines by using molecular makers is not affected by a growth period and an environment, can remarkably improve the breeding efficiency of the raphanus sativus L. CMS lines, and accelerates the breeding progress of excellent F1 hybrids.

Description

technical field [0001] The invention discloses a method for cultivating radish CMS male sterile lines by using molecular marker-assisted selection, belongs to the technical field of crop breeding, and is used for efficient cultivation of excellent CMS male sterile lines in radish heterosis utilization. Background technique [0002] Radish (Raphanus sativus L.), also known as radish and reed, belongs to the genus Radish of Cruciferae. It is an important root vegetable crop native to my country. It has a long history of cultivation in my country, with various types and wide distribution; radish is mainly edible The part is a fleshy root, which is not only rich in nutrition, but also has high medicinal and therapeutic value, and is deeply loved by people (Edited by Wang Longzhi and He Qiwei, Chinese Radish, Science and Technology Literature Publishing House, 2005). [0003] Male sterility refers to the inability of plants to produce functional pollen or male gametes, and can be ...

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to develop a method for assisted selection of radish candidate maintainer lines by using molecular markers in order to solve the problem of large workload and time-consuming screening and identification of candidate maintainer lines in the current breeding process of radish CMS male sterile lines. Accurately select excellent candidate maintainer lines, thereby improving the breeding process of excellent CMS male sterile lines in radish, and providing strong technical support for rapid breeding of large-scale excellent male sterile lines

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