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Compound gene chip for detecting salmonella serotype and detection method thereof

A Salmonella and gene chip technology, which is applied to the composite gene chip for detecting Salmonella serotypes and the field of detection thereof, can solve the problems that there are no national standards for Salmonella serotype detection and inspection and quarantine industry standards, etc., and achieves good sensitivity and high detection throughput. , a strong specific effect

Inactive Publication Date: 2011-01-26
中华人民共和国珠海出入境检验检疫局
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AI Technical Summary

Problems solved by technology

[0007] At present, there is no high-throughput, multi-gene rapid detection and serotype identification of Salmonella using gene chip technology, and there is no national standard for Salmonella serotype detection and industry standards for inspection and quarantine.

Method used

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  • Compound gene chip for detecting salmonella serotype and detection method thereof
  • Compound gene chip for detecting salmonella serotype and detection method thereof
  • Compound gene chip for detecting salmonella serotype and detection method thereof

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Embodiment Construction

[0064] In a specific embodiment, the mentioned BW, TMB, washing solution A, washing solution B, chromogenic solution and the instruments used are the biosensor gene chip system from Zhuhai Seleqi Biotechnology Co., Ltd.

[0065] 1. Design of target gene probes and primers

[0066] According to the similarities and differences of the genomes of the above seven Salmonella serotypes, eight target genes, including viaB, tyv, fliC-α, fliC1, rfbJ, fliC2, invA, and hilA, were selected. The correspondence between the seven Salmonella serotypes and the eight target genes is as follows: Table 1; Then, corresponding primers and probes were designed according to the eight target genes, the relevant information of the primers and probes is shown in Table 2, and the relevant information of the target genes is shown in Table 3.

[0067] Table 1 Correspondence between Salmonella serotypes and target genes

[0068]

[0069] * "√" indicates the target genes contained in the seven Salmonella...

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Abstract

The invention relates to a compound gene chip for detecting salmonella serotype and a detection method thereof. salmonella paratyphi A, salmonella paratyphi B, salmonella typhimurium, salmonella paratyphi C, salmonella typhisuis, salmonella typhi and salmonella enterica can be identified by integrating probes for detecting salmonella serotype target genes viaB, tyv, filiC-alpha, fliCl, rfbJ, fliC2, invA and hilA on the same chip. The invention can rapidly and accurately carry out the preliminary screening and identification of salmonella and serotype thereof, greatly shortens detection time and is applicable to the requirement of high throughput detection.

Description

【Technical field】 [0001] The invention relates to a gene chip, in particular to a composite gene chip for detecting Salmonella serotype and a detection method thereof. 【Background technique】 [0002] Salmonella is a group of bacteria that parasitize in the intestinal tract of humans and animals and can cause food poisoning and symptoms such as gastroenteritis, typhoid fever and paratyphoid fever. . In addition to infecting humans, they can also infect many animals including mammals, birds, reptiles, fish, amphibians and insects. Humans can be asymptomatic carriers after infection, or have clinical symptoms. Since 1985, the number of confirmed human illnesses caused by foodborne Salmonella has increased significantly worldwide. In 1999, Salmonella illnesses caused by eating ice cream occurred in the United States, with 22,400 people sick; in 2002, a group of Eggs exported from Spain contained salmonella, which caused the worst disease infection in British history; on Septe...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/10
CPCY02A50/30
Inventor 冯家望王小玉胡松楠成晓维游淑珠邝筱珊周新朋
Owner 中华人民共和国珠海出入境检验检疫局
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