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Saccharothrix used for preventing and controlling tomato leaf mold and preparation method thereof

A technology of tomato leaf mold and saccharomyces, applied in the field of saccharomyces for preventing and treating tomato leaf mold and its preparation

Inactive Publication Date: 2011-01-26
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]In-dish antagonism tests of antagonistic bacteria against tomato leaf mold are relatively common, but there are not many examples of using antagonistic bacteria to control tomato leaf mold. Even fewer biocontrol agents

Method used

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  • Saccharothrix used for preventing and controlling tomato leaf mold and preparation method thereof
  • Saccharothrix used for preventing and controlling tomato leaf mold and preparation method thereof
  • Saccharothrix used for preventing and controlling tomato leaf mold and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0034] Embodiment 1: Isolation and identification of Hhs.015 bacterial strain

[0035]Cucumber roots were collected from vegetable greenhouses in Yangling High-tech Demonstration Zone, Shaanxi, rinsed with tap water, and then dried. Take 1 g of the root samples of the plants and carry out surface disinfection (immerse in 99% ethanol for 1 min, then soak in 3.125% NaOCl). 6min, put it into 99% ethanol again for 30sec, and finally rinse it with sterile water for 5 times). Take 200 μL of the sterile water that has been rinsed for the last time and spread it on the Gaoshi No. 1 plate to test whether the surface disinfection is thorough. The decontaminated materials were cut into 0.5×0.5mm thin slices with a sterile scalpel, and the surface-sterilized cucumber root tissue pieces were placed on a Gaoshi No. 1 plate, cultured in an incubator at 28±1°C for 21 days and counted The number of Saccharomyces colonies was purified and the Hhs.015 strain could be isolated, and the obtained ...

Embodiment 2

[0046] Embodiment 2: the preparation method of Hhs.015 bacterial strain thalline

[0047] Inoculate the Saccharomyces yangling Hhs.015 strain preserved at -80°C on Gao’s No. 1 medium, culture it at 28°C for 4 days, inoculate its single colony into 100mL soybean flour liquid culture medium, and inoculate it at 28°C, 150rpm in the dark Shake culture for 48 hours, then inoculate 100mL soybean flour liquid culture solution according to 1% seed liquid, shake and culture in the dark at 28°C and 150rpm for 120h, centrifuge the fermentation liquid at 5000rpm for 20min, and the precipitate obtained is Saccharomyces yanglingensis Hhs .015 strain of bacteria.

[0048] Described soybean powder liquid culture solution is made of soybean powder 20g (boiled in water for 30min, filter and retain the filtrate), sucrose 10g, soluble starch 5g, peptone 2g, yeast extract 2g, NaCl 2g, CaCO 3 1g, MgSO 4 ·7H 2 O0.5g, KH 2 pO 4 0.5g, agar 15g, H 2 O 1000mL composition, and the pH value is 7.2. ...

Embodiment 3

[0049] Embodiment 3: the preparation method of the antibacterial active substance of Hhs.015 bacterial strain

[0050] Inoculate the Saccharomyces yangling Hhs.015 strain preserved at -80°C on Gao’s No. 1 medium, culture it at 28°C for 4 days, inoculate its single colony into 100mL soybean flour liquid culture medium, and inoculate it at 28°C, 150rpm in the dark Shake culture for 48 hours, then inoculate 100 mL of soybean flour culture solution with 1% seed liquid, shake and culture in the dark at 28°C and 150 rpm for 120 hours, centrifuge the obtained bacterial liquid at 5000 rpm for 20 minutes, and then concentrate the supernatant obtained.

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Abstract

The invention discloses Saccharothrix yanglingensis Hhs.015. The bacterial strain has been preserved in a China General Microbiological Culture Collection Center in June, 2009 with a collection number of CGMCC NO.4.5627; and the bacterial strain also has been preserved in a Type Culture Collection Center of the Korea Institute of Bioscience and Biotechnology in October, 2009 with a collection number of Saccharothrix yanglingensis Hhs.015T / KCTC 19722T. The invention also discloses a separation and identification method for the bacterial strain, a preparation method for thallus and a preparation method for crude antibacterial active extracts, and also discloses application of the Hhs.015 bacterial strain and the thallus and antibacterial active substances thereof in preventing and controlling the tomato leaf mold. The Hhs.015 bacterial strain has the advantages of stable natural raw material source, simple screening method, convenient operation, stable product quality, no toxic or side effect, environmental protection, obvious effect of preventing and controlling the tomato leaf mold, and convenient popularization and application.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and relates to a new bacterial strain for preventing and treating tomato leaf mold, in particular to a saccharomyces for preventing and controlling tomato leaf mold and a preparation method thereof. Background technique [0002] Tomato leaf mold (Fulvia fulva (Cook) Ciferri), commonly known as "black hair disease", is a common disease in tomato production, especially in protected areas. Once the disease occurs, it spreads rapidly and brings great harm to tomato production. a huge loss. Tomato leaf mold is a typical epidemic disease caused by fungi of the subphylum Deuteromycotina, which has the characteristics of intermittent outbreaks. In addition to causing leaf lesions and affecting photosynthesis, the pathogen can also infect stems and fruits, affect fruit quality, reduce edible value, and cause economic losses. In recent years, due to the expansion of tomato planting area in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/01
Inventor 黄丽丽颜霞康振生姚敏高小宁涂璇王心选
Owner NORTHWEST A & F UNIV
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