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Method for extracting and refining rhizobia exocellular polysaccharide

An extracellular polysaccharide and purification method technology, applied in the biological field, can solve the problems of high extraction cost and many steps, and achieve the effects of high purity, few process steps and high efficiency

Active Publication Date: 2012-03-28
天津强微特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the disadvantages of many steps, high extraction cost, and the finished product contains neutral and alkaline polysaccharides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, the method for extracting and refining Rhizobium gum from Rhizobium fermented liquid is completed by following processing steps:

[0031] Preparation of fermented liquid: the fermented strain adopts the rhizobium bacterium Rhizobium astragula (CICC: 20026), and the slant medium formula: beef extract 0.5%, peptone 3.9%, glucose 0.1%, sodium chloride 0.5%, agar 1.5-2.0 %, pH 7.2, culture temperature 30°C; shaker seed medium is prepared in the same way as above except that agar is not added; fermentation medium formula: NaCl0.01%, glucose 2%, K 2 HPO 4 0.4%, MgSO 4 .7H 2 O 0.5%, soybean meal 2%, Na 2 Mo 4 .2H 2 O0.002%, pH 7.0, culture temperature 30 DEG C; after seed culture 18 hours, connect fermenter by 5% inoculum amount, cultivate after 40 hours, kill enzyme through 60 DEG C for 30 minutes, put the tank to obtain fermented liquid.

[0032] 1. Dilution pretreatment:

[0033] Add 3 times the volume of distilled water to the fermentation broth, use 4...

Embodiment 2

[0045] The method for extracting and refining rhizobia gum from the rhizobia fermentation broth is completed by the following process steps:

[0046] Preparation of the fermented liquid: the fermented strain adopts Rhizobium radiobacter (CICC: 10270), the slant medium formula: 5 grams of peptone, 3 grams of beef extract, 5 grams of sodium chloride, 15 grams of agar, and 1000 milliliters of distilled water to adjust the pH to 7.0; The shaker seed medium is prepared in the same way as above except that agar is not added; the fermentation medium formula: NaCl0.01%, glucose 2%, K 2 HPO 4 0.4%, MgSO 4 .7H 2 O 0.5%, soybean meal 2%, Na 2 Mo 4 .2H 2 O0.002%, pH7.0, culture temperature 30 DEG C; After seed culture 20 hours, insert fermenter by 3% inoculum amount, cultivate after 48 hours, kill enzyme through 60 DEG C for 30 minutes, put the tank to obtain fermented liquid.

[0047] 1. Dilution pretreatment

[0048] Add 2 times the volume of distilled water to the fermentation bro...

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PUM

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Abstract

The invention discloses a method for extracting and refining rhizobia exocellular polysaccharide. The method is finished by the following steps: dilution pretreatment, flocculation and protein removal, ion exchange, concentration and salt removal, alcohol precipitation with alcohol, and vacuum drying. The method for extracting and refining the rhizobia exocellular polysaccharide from rhizobia fermentation liquor has the characteristics of few process steps, high efficiency, low cost and suitability for industrial production; and the prepared exocellular polysaccharide has high purity.

Description

technical field [0001] The invention relates to a method for extracting and refining rhizobium exopolysaccharide from fermented liquid of rhizobium, belonging to the field of biotechnology. Background technique [0002] Microbial polysaccharides are biopolymers produced by microorganisms such as bacteria, fungi, and cyanobacteria during the metabolic process that protect microorganisms. According to different forms of existence in cells, they are usually divided into three categories: Inner polysaccharides; ② cell wall polysaccharides adhered to the cell surface; ③ exopolysaccharides secreted into the culture medium, including microcapsules, capsules, mucus layer and bacterial micelles. Microbial polysaccharides have a wide range of applications, such as microbial flocculants in many fields of industrial production and life, biochemical profile control agents in microbial oil recovery, food additives, preservatives, anticancer drugs, packaging materials, etc. [0003] Rhizo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C12R1/41C12P19/04
Inventor 郑春阳魏国祥张巍
Owner 天津强微特生物科技有限公司
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