Stevia rebaudiana Bertoni extract and extraction method thereof and extraction method of rebaudioside A

An extraction method and technology of stevia rebaudiana, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of inability to separate and obtain rebaudioside A

Inactive Publication Date: 2011-01-12
成都华高瑞甜科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The inventor's repeated experiments have shown that such a simple treatment step cannot separate rebaudioside A from other components that are very similar in structure, so in practice, the claimed purity of 99% rebaudioside A cannot be obtained through this patented method Diglycoside A

Method used

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  • Stevia rebaudiana Bertoni extract and extraction method thereof and extraction method of rebaudioside A
  • Stevia rebaudiana Bertoni extract and extraction method thereof and extraction method of rebaudioside A
  • Stevia rebaudiana Bertoni extract and extraction method thereof and extraction method of rebaudioside A

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 The influence of stevia rebaudiana raw material and solvent ratio on continuous countercurrent extraction

[0034] According to the weight-to-volume ratio of 1:3, 1:5, 1:7, 1:9 (m / v), 10 kg of dried Stevia rebaudiana (Stevia rebaudiana Bertohi) leaves and stems (containing 8% rebaudioside A ) and 5% (v / v) mixed solvent of water and methanol at 70°C for continuous countercurrent extraction, and the flow rate of the mixed solvent of water and methanol for every kilogram of stevia raw material is 2L / h.

[0035] Measure the volume of the extract, and quantitatively measure part of the extract, evaporate to dryness to constant weight, weigh, and take samples respectively for purity identification by HPLC. The experimental results are shown in Table 1.

[0036] The influence of table 1 stevia rebaudiana raw material and solvent ratio on continuous countercurrent extraction

[0037]

[0038] The above experimental results show that in the continuous countercurre...

Embodiment 2

[0040] Embodiment 2 The influence of water and methanol mixed solvent ratio on continuous countercurrent extraction

[0041] 10 kg of Stevia rebaudiana Bertohi leaves and stems were mixed with 0%, 5%, 10%, 15% (v / v) water and methanol at a weight-to-volume ratio of 1:6 (m / v) The solvent is subjected to continuous countercurrent extraction at 70° C., and the flow rate of the mixed solvent of water and methanol for each kilogram of stevia raw material is 2 L / h.

[0042] Measure the volume of the extract, and quantitatively measure a part of the extract, evaporate it to constant weight, weigh it, and take samples for purity identification by HPLC. The experimental results are shown in Table 2.

[0043] Table 2 The influence of water and methanol mixed solvent ratio on continuous countercurrent extraction

[0044]

[0045] The above experimental results show that in the continuous countercurrent extraction of stevia rebaudiana raw materials, when the mixed solvent ratio of wat...

Embodiment 3

[0047] Embodiment 3 The influence of extraction temperature on continuous countercurrent extraction

[0048] According to the weight-to-volume ratio of 1:6 (m / v), 10 kilograms of stevia (Stevia rebaudiana Bertohi) leaves and stems were mixed with 7% (v / v) of water and methanol at 50°C, 60°C, and 70°C respectively. ℃, 80 ℃ for continuous countercurrent extraction, the flow rate of water and methanol mixed solvent per kilogram of stevia raw material is 2L / h.

[0049] Measure the volume of the extract, and quantitatively measure a part of the extract, evaporate to dryness to constant weight, weigh it, and take samples respectively for purity identification by HPLC. The experimental results are shown in Table 3.

[0050] Table 3 The influence of extraction temperature on continuous countercurrent extraction

[0051]

[0052] The above experimental results show that in the continuous countercurrent extraction of Stevia rebaudiana raw materials, the best extraction effect is ach...

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Abstract

The invention relates to an extraction method of rebaudioside A, a Stevia rebaudiana Bertoni extract and an extraction method thereof, belonging to the food chemical industry field. The invention aims to provide the method for extracting the high-purity and high-yield rebaudioside A from Stevia rebaudiana Bertoni. The method comprises the following steps: a. carrying out continuous countercurrent extraction on leaves and stem of the Stevia rebaudiana Bertoni and a water-methanol mixed solvent at 50-80 DEG C based on the proportion of 1:3-1:9m / v, wherein, flow velocity of the water-methanol mixed solvent on a Stevia rebaudiana Bertoni raw material per kilogram is 1-4L / h; b. concentrating the obtained extracting solution to obtain a crude rebaudioside A product, dispersing the crude product with water, and then ultra-filtering to obtain filtrate; c. adsorbing and eluting the obtained ultrafiltrate with polar resin, and then eluting with the water-methanol mixed solvent; and d. concentrating the obtained eluent, freezing, stirring and crystallizing the obtained concentrated solution at the temperature of -20 DEG C for 24 hours, freezing, stirring and recrystallizing at the temperature of -20 DEG C for 24 hours, collecting and drying crystal at 80 DEG C to obtain the rebaudioside A with purity up to not less than 99% and yield up to more than 85%. The obtained rebaudioside A has no adverse remaining taste on mouthfeel, large treatment capacity and low cost, thus being applicable to industrial production.

Description

[0001] This application is a divisional application of the invention patent application with the application number: 200610022507.2, the invention name: rebaudioside A and its extraction method, and the application date is December 15, 2006. technical field [0002] The invention relates to an extraction method of rebaudioside A, a stevia rebaudiana extract and the extraction method thereof, and belongs to the field of food chemical industry. Background technique [0003] Stevioside (Total steviosides) is a high-sweetness, low-calorie natural health-care sweetener extracted from the leaves and stems of Stevia rebaudiana Bertohi. It mainly contains eight different sweet components: stevioside (Stevioside); Rebaudioside (Rebaudioside) A, B, C, D, E and dulcoside (Dulcoside) A, B. Among them, stevioside accounts for about 66% of the total glycosides, rebaudioside A accounts for about 22% of the total glycosides, rebaudioside C accounts for about 9% of the total glycosides, and ...

Claims

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Application Information

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IPC IPC(8): C07H15/256C07H1/08
Inventor 阳明福华君秦岭许庆
Owner 成都华高瑞甜科技有限公司
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