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Method for constructing congenital heart disease with diminutive pulmonary blood animal model

A technology of animal model and construction method, applied in the fields of medical technology and experimental zoology, to achieve the effect of small trauma, avoid large trauma, and reduce surgical mortality

Inactive Publication Date: 2011-01-12
蚌埠医学院附属医院
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Problems solved by technology

[0004] There are many problems in the animal models constructed by the predecessors. So far, there is no ideal animal model of congenital heart disease with reduced pulmonary blood flow. We have explored a novel hybrid (Hybird) technology through the pre-experimental research on the construction of animal models in young pigs. , the method of closed balloon dilation of artificial atrial septostomy + surgical pulmonary artery constriction successfully constructed a piglet animal model of congenital heart disease with reduced pulmonary blood flow that is closer to the clinical pathophysiological state, aiming to provide a basis for the treatment of this type of congenital heart disease. Basic research provides a stable and reliable animal model

Method used

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  • Method for constructing congenital heart disease with diminutive pulmonary blood animal model
  • Method for constructing congenital heart disease with diminutive pulmonary blood animal model
  • Method for constructing congenital heart disease with diminutive pulmonary blood animal model

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Experimental program
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Embodiment 1

[0026] Embodiment 1: Construction of animal model

[0027] 1. Experimental animals and anesthesia:

[0028] 20 Chinese miniature piglets, male or female, aged 1-2 months, weighing 10-12kg, were purchased from the Experimental Animal Breeding Center of Fudan University in Shanghai, license number: SCXK (Shanghai) 2007-0013. Randomly divided into 3 groups: control group (group C n=6), mild to moderate pulmonary artery stenosis group (T1 group n=7), severe pulmonary artery stenosis group (T2 group n=7).

[0029] After preoperative intramuscular injection of ketamine (10 mg / kg) and midazolam (0.5 mg / kg) for basic anesthesia and tranquillization, venous access was established by ear vein puncture, femoral artery or brachial artery puncture was connected to invasive blood pressure monitoring, and chest leads were connected to electrocardiogram monitoring. Anesthesia-induced endotracheal intubation and intravenous anesthesia, connected to an imported pediatric ventilator, inhaled ox...

Embodiment 2

[0032] Example 2: Evaluation of Observation Index and Model Establishment

[0033] 1. Blood gas analysis and blood routine. Femoral artery blood gas analysis was taken before operation, at the end of operation, and at 1 month and 2 months after operation for pH value and partial pressure of oxygen (PaO 2 ), carbon dioxide partial pressure (PCO2) and oxygen saturation (SaO2); venous blood was drawn to measure hemoglobin content (Hb) and hematocrit (Hct). See Table 1, Table 2, Table 3.

[0034] 2. Ultrasound observations Sonos-5500 color Doppler ultrasonic diagnostic instrument and Philips T6207 esophageal ultrasound probe from Philips Company of the United States were used. All experimental animals underwent premenstrual chest wall echocardiography before operation, at the end of operation, and at 1 month and 2 months after operation. Intraoperative ultrasonography of the esophagus was performed to eliminate differences within the group. see figure 1 shown.

[0035] 3. The ...

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Abstract

The invention discloses a method for constructing a congenital heart disease with diminutive pulmonary blood animal model, which is characterized in that: a surgical knife enters a fourth intercostal of right chest lateral incision, and a pericardium is opened and suspended; an interatrial septum is directly expanded by a puncture needle and a balloon dilator from the right chest lateral under ultrasound guidance to form an atrial septal defect with the diameter of 1.0cm; a main pulmonary artery is sleeved by an artificial blood vessel sheet to monitor peripheral arterial blood pressure and differential pressure of a girdle place, and then the differential pressure is decreased gradually; and when the differential pressure of the girdle place of mild-moderate pulmonary stenosis is 20 to 30mmHg, or the differential pressure of the girdle place of severe pulmonary stenosis is more than or equal to 30mmHg, and the arterial blood pressure is kept stable, a girdle belt is fixed so as to form the congenital heart disease with diminutive pulmonary blood animal model. The method has the advantages of exactness, feasibility, simple and reliable operation method, convenient operation and a few complications; and the constructed animal model is more ideal and better adapts to changes of clinical pathophysiology of the disease.

Description

technical field [0001] The invention belongs to the fields of medical technology and experimental zoology, and relates to a method for constructing an animal model of congenital heart disease with reduced pulmonary blood flow. Background technique [0002] Congenital heart diseases with diminutive pulmonary blood include tetralogy of Fallot (TOF), tricuspid atresia (TA), pulmonary atresia (PAA) and other complexes with pulmonary artery stenosis (PS) Cardiac malformations, such as double outlet right ventricle (DORV), complete transposition of the great arteries (D-TGA), corrected transposition of the great arteries (C-TGA), single ventricle (SV) and functional single ventricle (FSV), etc. The common pathophysiological manifestations are preoperative pulmonary vascular dysplasia, significantly reduced pulmonary blood flow, and low pulmonary arterial pressure. After dredging the right ventricular outflow tract in such diseases, a large amount of blood enters the underdevelope...

Claims

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Application Information

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IPC IPC(8): A61B19/00A61B90/00
Inventor 刘学刚刘戈张雷王康武施超
Owner 蚌埠医学院附属医院
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