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Method for detecting cattle ANGPTL4 gene single nucleotide polymorphism

A single nucleotide polymorphism, gene technology, used in biochemical equipment and methods, microbial determination/inspection, etc.

Inactive Publication Date: 2010-12-22
NORTHWEST A & F UNIV
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Problems solved by technology

There is no research on the genetic variation of cattle ANGPTL4 gene at home and abroad

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  • Method for detecting cattle ANGPTL4 gene single nucleotide polymorphism
  • Method for detecting cattle ANGPTL4 gene single nucleotide polymorphism
  • Method for detecting cattle ANGPTL4 gene single nucleotide polymorphism

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Embodiment Construction

[0026] The present invention detects the single nucleotide polymorphism that may result in a change in the conformation of the encoded protein due to the missense mutation at the 1422nd position of the yellow cattle ANGPTL4 gene, so as to be used for the marker-assisted selection of the growth traits of Chinese yellow beef, and to quickly establish genetic resources Excellent cattle population. In the following, the present invention will be further described in detail in combination with the detection of specific samples and the association of traits, which is an explanation of the present invention rather than a limitation.

[0027] a. Design of PCR primers for the third exon region of cattle ANGPTL4 gene

[0028] Taking the bovine (NC 007305) sequence published by NCBI as a reference, Primer 5.0 was used to design PCR primers capable of amplifying the ninth exon region of the cattle ANGPTL4 gene. The primer sequences are as follows:

[0029] Upstream primer P1: tgcgaatcca ...

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Abstract

The invention discloses a method for detecting cattle ANGPTL4 gene single nucleotide polymorphism, comprising the following steps of: carrying out PCR proliferation on a cattle ANGPTL4 gene by using a cattle whole-genome DNA to be detected as a template and using a primer pair P as a primer, wherein the cattle whole-genome DNA to be detected includes an ANGPTL4 gene; digesting a product subjected to PCR proliferation by using restriction enzyme MspI, and carrying out polyacrylamide gel electrophoresis on a proliferated segment subjected to enzyme cutting; and authenticating the polymorphism of the single nucleotide in the 1422nd position of the cattle ANGPTL4 gene according to the result of the polyacrylamide gel electrophoresis. Because the ANGPTL4 gene function relates to four important growth characteristics of weight, daily gain, body slanting length and chest circumference, the method provided by the invention lays the foundation for establishing the relation of SNP (Single Nucleotide Polymorphism) of ANGPTL4 gene and the growth characteristics so as to be convenient for mark assisted selection (MAS) of the growth characteristic for Chinese cattle meat and rapidly establishes cattle species groups with excellent genetic resources.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to the detection of gene single nucleotide polymorphism (SNP), in particular to a method for detecting the single nucleotide polymorphism of cattle ANGPTL4 (angiogenic growth factor 4) gene. Background technique [0002] Gene polymorphism refers to the difference in genome sequence between different species or different individuals within the same species. These differences are caused by nucleotide changes in DNA alleles in chromosomes, mainly including base substitutions, insertions, deletions and duplications. Changes in sequence copy number. [0003] Single nucleotide polymorphism (Single Nucleotide Polymorphism, SNP) is a type of genetic marker system proposed by Lander (1996), a scholar at the Human Genome Research Center of the Massachusetts Institute of Technology. Polymorphism caused by acid (A / T / C / G) substitution. Its variant forms include: transversion, conversion, insertion an...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 陈宏侯飞马云李荣荣朱金龙蓝贤勇
Owner NORTHWEST A & F UNIV
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