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Method for acquiring genetic information of ancient animal

A technology of genetic information and extraction method, which is applied in the field of ancient animal DNA extraction and acquisition of ancient animal genetic information, can solve the problems of inability to obtain ancient DNA target sequences, discount of DNA fragment amplification efficiency, and inability to obtain genetic information, etc., to achieve reduction The effect of amplifying interfering substances, low cost, and high extraction efficiency

Inactive Publication Date: 2010-12-22
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] At present, the common methods for ensuring the authenticity of ancient DNA genetic information mainly focus on the pre-treatment of samples. Since the content of ancient DNA in samples is extremely rare, if the inhibitors and interferences contained in samples cannot be effectively removed during the extraction process , the amplification efficiency of the resulting DNA fragments will often be greatly reduced, and some measures to remove contamination may lead to a reduction in extraction efficiency, so that the desired ancient DNA target sequence cannot be obtained. Measures to exclude the interference of damaged DNA sequence information will also fail to obtain the required genetic information

Method used

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  • Method for acquiring genetic information of ancient animal
  • Method for acquiring genetic information of ancient animal
  • Method for acquiring genetic information of ancient animal

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Embodiment 1

[0053] Embodiment 1, the acquisition of ancient animal genetic information

[0054] 1. Preparation of DNA Extraction Solution

[0055] 1. Obtain DNA extract by the time-delayed precipitation extraction method at room temperature provided by the present invention

[0056] 1) Experiment preparation

[0057] ①Laboratory

[0058] All processes prior to PCR amplification (i.e., the process of preparing DNA extracts) were performed in an independent ancient DNA laboratory that never allowed access to modern animal DNA or amplified DNA. The instruments and equipment used are all dedicated to the operation of ancient DNA, and the corrosion-resistant experimental instruments and equipment are regularly diluted with bleach (the active ingredient is sodium hypochlorite) (1:8) to remove residual nucleic acid contamination. The water used in the laboratory was ultrapure water filtered twice, once filtered through an ultrapure water meter (Millipore, USA), and secondly filtered through a...

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Abstract

The invention discloses a method for acquiring the genetic information of an ancient animal. The invention also provides a method for extracting the DNA of the ancient animal. The method for extracting the DNA of the ancient animal comprises the following steps of: (1) putting an ancient animal sample in a cracking buffer solution for cracking and centrifuging to obtain a cracking solution; (2) adding a settling aid buffer solution with volume equal to that of the cracking solution to the cracking solution and centrifuging to obtain a supernatant; and (3) taking the supernatant, adding isopropyl alcohol with volume equal to that of the supernatant to the supernatant and centrifuging to obtain a DNA precipitate. On the basis of the step (3), the method for acquiring the genetic information of the ancient animal also comprises the following steps of: (4) washing; (5) dissolving the DNA precipitate obtained in the step (4) with water and carrying out amplification and sequencing after damaged templates are removed; and (6) carrying out multiple conventional amplification and sequencing by sampling, and then comparing with a result of the step (5) to acquire real genetic information of the ancient animal. The methods of the invention have the characteristics of simplicity, time saving, low cost and high extraction efficiency and is suitable for detection works with large sample quantities.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a method for extracting ancient animal DNA and a method for obtaining ancient animal genetic information. Background technique [0002] Ancient DNA (ancient DNA, aDNA) refers to the DNA obtained from the remains and remains of dead ancient animals. In the past ten years, the molecular biology of ancient animals has developed rapidly. People have used animal remains to carry out a large number of DNA extraction and research, and successfully obtained a large number of genetic information of extinct animals, revealing the system among these extinct animals. Genetic relationship, domestication, distribution and dispersal information can also be obtained through the analysis of genetic information of domestic animal remains, which provides evidence for human development and migration. [0003] Generally speaking, due to the long history and the influence of internal and external env...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10C12Q1/68
Inventor 赵兴波方迟
Owner CHINA AGRI UNIV
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