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Process for preparing nutritional, therapeutic or organoleptic products from crude glycerol

A therapeutic and nutritional technology, applied in the direction of microorganism-based methods, methods using microorganisms, biochemical equipment and methods, etc., can solve problems such as the difficulty of large-scale implementation feasibility

Inactive Publication Date: 2010-12-15
BIO PROCESSING AUSTRALIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the feasibility of implementing these methods on a large scale has proven elusive, and therefore, there remains a need for viable methods that can convert excess crude glycerol into high-value products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1 : Process crude glycerin

[0064] Crude glycerol, a by-product from biodiesel production, is heated to about 25°C to liquefy the material. The crude glycerol has a pH of about 10.0 and a methanol content of less than 1% by weight. Crude glycerol (1000 L) was transferred to a 1300 L stainless steel tank equipped with overhead stirring and heating / cooling jacket. After warming to 60°C, 85% by weight phosphoric acid solution was slowly pumped into the stirred mixture until an endpoint pH 4.0 was reached as determined by titration. Additional water (250 L) was added to keep the salts formed in solution. Subsequently, the mixture was allowed to stand for 2 hours, after which the lower aqueous glycerol phase (TCG) was separated and stored for use in the fermentation stage. Alternatively, the mixture can be centrifuged to obtain upper and lower phases, which are then separated. The glycerol content in the TCG phase was about 700 g / l. Before further use, TCG wa...

Embodiment 2

[0071] Example 2 : In the presence of processed crude glycerol in Example 1, continuous fermentation of Candida utilis

[0072] A 2L laboratory aerobic fermenter was set up in a continuous manner with facilities for controlling pH as well as temperature and foam, as well as measuring dissolved oxygen (DO), supplying fermentation medium and collecting culture. Oxygen was supplied to the fermenter as needed in order to prevent oxygen starvation of the continuous culture if necessary.

[0073] The medium supplied to the fermentor consists of the following three components:

[0074] (1) The treated crude glycerol (as sole carbon source) of Example 1 with a glycerin concentration of about 500 g / L and a pH of 4.0, provided at a flow rate of 50 ml / hour;

[0075] (2) (i) Mineral salt concentrate (containing S, K, P, Mg, Na, Ca, Zn, Fe, Mn, Co, Cu, Mo and B), mixture of (ii) sulfuric acid and (iii) phosphoric acid , the mixture has a pH of 0.8 and is provided at a flow rate of 110 ...

Embodiment 3

[0087] Example 3 : Large-scale continuous fermentation of Candida utilis in the presence of the processed crude glycerol of Example 1.

[0088] Set up a 500 liter laboratory aerobic fermenter in continuous mode with facilities for controlling pH as well as temperature and foam, as well as measuring dissolved oxygen (DO), supplying fermentation medium and collecting culture.

[0089] The components in the medium include the processed crude glycerol of Example 1 (as the sole carbon source), mineral salt concentrate (containing S, K, P, Mg, Na, Ca, Zn, Fe, Mn, Co, Cu , Mo and B), phosphoric acid, sulfuric acid and water. Appropriate amounts of all these components were premixed in 5000 liter batches to achieve the concentrations shown in Table 2 below. The combined medium is then pasteurized at 90°C for 60 to 90 minutes before being sent to the fermenter. The pH of the pasteurized combined medium was about 1.8. On a larger scale, media components such as crude glycerol, mine...

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Abstract

The present invention relates to a process for preparing a nutritional, therapeutic o organoleptic product by growing non-recombinant yeast under aerobic conditions, in medium that includes crude glycerol, as one possible carbon source to produce a yeas product. The yeast product can be processed to obtain such nutritional, therapeutic o organoleptic products as yeast paste, yeast metabolites, carbohydrates, proteins functional proteins, nucleotides, yeast autolysates, yeast extract, yeast cell walls, beta glucans, mannans or a product derived from a mineralized yeast product.

Description

technical field [0001] The present invention relates in particular, but not exclusively, to a method of fermenting yeast using crude glycerol as a feedstock for the manufacture of nutritional, therapeutic and / or sensory products. Background technique [0002] Crude glycerin is available in large quantities as a by-product of processes such as soap and detergent production, alcoholic beverage production, fatty acid manufacture, and biodiesel production. Biodiesel is a fuel derived from vegetable oils or animal fats that is used in diesel engines and heating systems. The fuel is renewable and non-toxic, and its use reduces harmful emissions. As a result, the manufacture of biodiesel has sprung up around the world, making it a compelling alternative to expensive and polluting petroleum-based fuels. In 2008, the United States National Biodiesel Board reported that in the United States alone, biodiesel production reached 2.24 billion gallons per year (see http: / / www.biodiesel....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A23J1/18A23L1/28
CPCC12P1/02A23K1/009C12N1/32A23L1/3016C12P19/00A61K36/064A23L1/30A23K1/008A61K36/06A23K1/1631A23K10/16A23K10/18A23K20/147A23L33/10A23L33/14C12R2001/645C12N1/145C12R2001/72C12N1/165C12R2001/85C12N1/185C12R2001/865Y02E50/10
Inventor 罗宾·费德豪斯唐诺·芬莱·麦克连南玛莉·伊丽莎白·麦克连南大卫·葛拉汉·麦克连南
Owner BIO PROCESSING AUSTRALIA
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