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Compound of prostate stem cell antigen and heat shock protein and preparation method and application thereof

A technology of stem cell antigen and heat shock protein, which is applied in the field of complexes of prostate stem cell antigen and heat shock protein, can solve the problems of androgen-independent lesions, inability to accurately distinguish prostate cancer, lack of specificity in diagnostic methods, etc., and achieve the goal of preparing The method is simple and the effect of good development and application prospects

Inactive Publication Date: 2010-12-15
ARMY MEDICAL UNIV
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  • Abstract
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Problems solved by technology

Although some progress has been made recently in the diagnosis and treatment of localized prostate cancer, there are still many problems to be solved, such as the lack of specificity of diagnostic methods, the inability to accurately distinguish between quiescent and progressive prostate cancer, and the dif

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  • Compound of prostate stem cell antigen and heat shock protein and preparation method and application thereof
  • Compound of prostate stem cell antigen and heat shock protein and preparation method and application thereof
  • Compound of prostate stem cell antigen and heat shock protein and preparation method and application thereof

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Embodiment Construction

[0019] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The experimental methods that do not specify specific conditions in the preferred embodiments usually follow conventional conditions, such as those described in the molecular cloning experiment guide (third edition, J. Sambrook et al., translated by Huang Peitang et al., Science Press, 2002) Or in accordance with the conditions recommended by the manufacturer.

[0020] 1. Preparation of PSCA-HSP70 complex

[0021] 1. Preparation of PSCA

[0022] (1) Cloning of full-length PSCA cDNA

[0023] According to the PSCA gene sequence with GenBank accession number NM_005672, the following primers were designed and synthesized to amplify the full length of PSCA cDNA: F1: 5'-atgaaggctgtgctgcttgccctgt-3' (SEQ ID No. 3), R1: 5'-cgagctggccgggtccccagagc-3' (SEQ ID No.4); Extract the total RNA of prostate cancer cell LNCaP, reverse transcribed into cDNA, and then ...

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Abstract

The invention discloses a compound of prostate stem cell antigen and heat shock protein and a preparation method and application thereof. The compound is prepared by combining the prostate stem cell antigen and the heat shock protein 70 with a non-covalent bond. Through the compound, the specificity CTL can be activated, powerful immunity against carcinoma of prostate can be induced without any adjuvant, and the application between the same species is not limited to MHC; meanwhile, the prostate stem cell antigen comprises a plurality of epitopes of class I molecules and class II molecules of the MHC, so CD8+ cells and CD4+T cells can be activated in a broad spectrum; and the prostate stem cell antigen also comprises T-cell epitopes and B-cell epitopes, so the antitumor response of the T cells and the B cells can be activated. The preparation method of the compound is simple, and the compound can be used for preparing the vaccines against the carcinoma of prostate and has broad development and application prospect in the field of immunotherapy for the carcinoma of prostate.

Description

Technical field [0001] The invention relates to a protein complex, in particular to a complex of prostate stem cell antigen and heat shock protein, and also to a preparation method and application of the complex. Background technique [0002] Prostate cancer is a common tumor in western countries and ranks the second cause of death from tumors in men. Although recent progress has been made in the diagnosis and treatment of localized prostate cancer, there are still many problems that need to be resolved, such as the lack of specificity of the diagnosis method, the inability to accurately distinguish between static and progressive prostate cancer, and it is difficult to predict the prognosis of patients. About 20%-40% of patients relapse after surgery and radiotherapy. Although androgen blockade therapy can relieve the condition of some patients, most patients inevitably develop androgen-independent lesions that cannot be treated at present. Therefore, the development of new met...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61K47/42A61P13/08A61P35/00
Inventor 杨曌吴玉章
Owner ARMY MEDICAL UNIV
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