Lactic acid bacterium or yeast porous starch embedding and protecting method

Abstract

The invention discloses a lactic acid bacterium or yeast porous starch embedding and protecting method. Lactic acid bacteria or yeasts are cultured in a fermentation tank with high density to make the total amount of the lactic acid bacteria in a fermentation liquor reach 3.4 to 4.5 * 10<9>CFU / mL and the total amount of the yeasts reach 2 to 3 * 10<10>CFU / mL; the fermentation liquor is concentrated by 15 to 20 times in vacuum to obtain a concentrated bacterial liquid; porous starch, dried whey and trehalose are added into the concentrated bacterial liquid; and after being uniformly stirred until the mixture is pasty, the mixture is dried to obtain a product with relatively high viable bacteria content. The amount of the viable bacteria in the lactic acid bacterium or yeast embedding preparation produced by the technique is over 10<10>CFU / g; the product is resistant in storage; the production process is simple; and the cost is low.

Description

technical field [0001] The invention relates to a preparation method of microbial preparations, in particular to a method for protecting lactic acid bacteria or yeast microecological preparations. Background technique [0002] Antibiotics once played an important role in animal husbandry, but their disadvantages such as drug residues, damage to the ecological environment, and drug resistance of pathogenic bacteria are becoming more and more obvious. With the improvement of people's living standards and the strengthening of food safety awareness, there is an urgent need for green feed additives to produce green livestock and poultry products. Lactic acid bacteria feed additives and yeast feed additives are important green feed additives. [0003] However, because yeast and most lactic acid bacteria do not produce spores, they have poor stress resistance and are easy to die, which seriously affects the use effect. For example, when lactic acid bacteria are added to feed pell...

AI Technical Summary

Problems solved by technology

However, these methods have high requirements on the selection of materials, the production process is relatively complicated, and the investment in equipment is large. At the same time, there are also disadvantages such as the number of viable bacteria is not high during drying.

[0005] At present, domestic microcapsules made of lactic acid bacteria and yeast are all made of sodium alginate, kana gum, cross-linked polyacrylamide, silica gel, carrageenan, polyvinyl alcohol gel, light Hardened resin, polyacrylic acid gel and other high-molecular substances are used as embedding materials, and porous starch is used as microbial encapsulation and preservation materials such as lactic acid bacteria and yeasts for embedding. There are few reports in China.