Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Benazepril hydrochloride composition with effect of reducing blood pressure and preparation method thereof

A technology of benazepril hydrochloride and its composition, which is applied in the field of benazepril hydrochloride composition and its preparation, and can solve the problems of restricting clinical application and unfulfilling control of hypertension, etc.

Inactive Publication Date: 2013-01-09
BEIJING WBL PEKING UNIV BIOTECH
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effects of antihypertensive drugs on metabolism and target organ damage are still controversial, and most of them have certain side effects, which partially limit their clinical application.
Make the control of high blood pressure unsatisfactory

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Benazepril hydrochloride composition with effect of reducing blood pressure and preparation method thereof
  • Benazepril hydrochloride composition with effect of reducing blood pressure and preparation method thereof
  • Benazepril hydrochloride composition with effect of reducing blood pressure and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1: Solid fermentation of the composition of benazepril hydrochloride

[0055] Slope strain culture: Aspergillus CICC 2436 is selected for strain culture, agar is added to wort at 4 degrees to prepare a medium with pH 7 so that each ml of medium contains 0.05g of agar; sterilize at 110°C, After sterilization, cool, prepare and inoculate the slant, cultivate at 30°C for 5 days as the slant strain; solid fermentation culture: mix the bran and wort in a weight-to-volume ratio of 1:1, and mix well Sterilize for 30 minutes at 110°C, cool after sterilization, and insert the slant strains so that the volume of the slant strains accounts for 8% of the culture medium. Incubate at 30°C for 3 days and add to the cultured bran Soak in 0.3M pH7 pbs buffer overnight to obtain a solid fermentation broth; preparation of crude enzyme solution: add ammonium sulfate to the solid fermentation broth until it is saturated, stir uniformly until completely dissolved, place overnight at 4°C...

Embodiment 2

[0056] Example 2: Solid fermentation of the composition of benazepril hydrochloride

[0057] Slope strain culture: Aspergillus CICC 2436 is selected for strain culture, agar is added to wort at 4 degrees to prepare a pH 6 medium, so that each ml of medium contains 0.03g of agar; sterilize at 105°C and kill After the bacteria are cooled, the slant is prepared and inoculated by conventional methods, and cultured at 35°C for 4 days as the slant strain; solid fermentation culture: mix the bran and the wort in a weight-to-volume ratio of 1:1.5, and mix well Then sterilize for 35 minutes at 105°C, cool after sterilization, and insert the slant strain so that the volume of the slant strain accounts for 6% of the volume of the medium. Cultivate at 35°C for 2 days. After the cultured bran Add 0.5M pH7pbs buffer and soak overnight to obtain solid fermentation broth; preparation of crude enzyme solution: add ammonium sulfate to solid fermentation broth until it is saturated, stir evenly unt...

Embodiment 3

[0058] Example 3: Solid fermentation of the composition of benazepril hydrochloride

[0059] Slope strain culture: Aspergillus CICC 2436 is selected for strain culture, agar is added to wort at 4 degrees to prepare a pH 7 medium, so that each ml of medium contains 0.08g of agar; sterilize at 115°C and kill After the bacteria are cooled, the slant is prepared and inoculated by conventional methods, and cultivated at 25°C for 6 days as the slant strain; solid fermentation culture: mix the bran and the wort in a weight-to-volume ratio of 1:1.8, and mix well Then sterilize for 25 minutes at 120°C, cool after sterilization, and insert the slant strain so that the volume of the slant strain accounts for 9% of the volume of the medium. Cultivate at 25°C for 2 days. After the cultured bran Add 0.4M pH7pbs buffer solution and soak overnight to obtain solid fermentation broth; preparation of crude enzyme solution: add ammonium sulfate to solid fermentation broth until it is saturated, stir...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses a benazepril hydrochloride composition with effect of reducing blood pressure and a preparation method thereof. The preparation process comprises a solid fermentation method and a liquid fermentation method. Aspergillus CICC 2436 produces enzyme to hydrolyze benazepril hydrochloride, wherein the structure of part of benazepril hydrochloride is changed, namely an analog of benazepril hydrochloride. The original benazepril hydrochloride forms a composition containing the benazepril hydrochloride and the analog of the benazepril hydrochloride under the action of the enzyme, and the level of reducing the blood pressure by using the composition is superior to that of the benazepril hydrochloride with the same concentration.

Description

Technical field [0001] The invention relates to a composition, in particular to a benazepril hydrochloride composition with a blood pressure lowering effect and a preparation method thereof. Background technique [0002] At present, there are roughly 6 types of drugs for the treatment of hypertension in modern medicine: 1. Diuretics 2. Alpha receptor blockers 3. Beta receptor blockers 4. Calcium antagonists 5. Angiotensin converting enzyme inhibitors 6 . Angiotensin II receptor antagonist. In recent years, with the development of large-scale antihypertensive clinical trials and the progress of cardiovascular molecular biology research, the traditional understanding of hypertension has been updated, and symptomatic medicine has become a consensus. Hypertension is not only a disease with abnormal hemodynamics, but also accompanied by disorders of fat and glucose metabolism and poor remodeling of target organs such as the heart, brain and kidney. Therefore, treatment should not on...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/10A61K31/55A61P9/12C12R1/66
Inventor 段震文郭树仁巩金妹薛岚刘春丽
Owner BEIJING WBL PEKING UNIV BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com