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Method for separating and preparing agarose from agar by using polyethylene glycol precipitation method

A technology of polyethylene glycol precipitation and polyethylene glycol, which is applied in the field of preparing a high gel strength, can solve the problems of poor gel properties, etc., and achieve the effects of low production cost, high yield, and good gel properties

Active Publication Date: 2010-11-24
泉州市海川生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing agar containing agar ester has poor gel properties

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for separating and preparing agarose from agar by the polyethylene glycol precipitation method of the present invention is realized through the following steps:

[0023] 1. Prepare 100mL of agar solution with a mass percentage concentration of 4%, put it on an electric stove, stir and heat to dissolve into a translucent solution;

[0024] 2. Put the completely dissolved agar solution into the water bath to keep the temperature constant at 80°C;

[0025] 3. Prepare 100mL of a polyethylene glycol 6000 solution with a concentration of 40% by mass, heat it in a water bath to completely dissolve it and keep the temperature constant at 80°C;

[0026] 4. Pour the polyethylene glycol 6000 solution into the agar solution at a volume ratio of 1:0.5 to 1:3 in a water bath at 80°C and quickly mix it. Stir continuously for about 2 minutes. A large amount of white flocculent precipitates, and a large amount of white flocculent is obtained. Suspension of floc precipitation; ...

Embodiment 2

[0034] A method for separating and preparing agarose from agar by the polyethylene glycol precipitation method of the present invention is realized through the following steps:

[0035] 1. Prepare 100mL of agar solution with a mass percentage concentration of 3%, put it on an electric stove, stir and heat to dissolve into a translucent solution;

[0036] 2. Put the completely dissolved agar solution into the water bath to keep the temperature constant at 80°C;

[0037] 3. Prepare 100mL of a polyethylene glycol 6000 solution with a concentration of 40% by mass, heat it in a water bath to completely dissolve it and keep the temperature constant at 80°C;

[0038] 4. Pour the polyethylene glycol 6000 solution into the agar solution at a volume ratio of 1:0.5 to 1:3 in a water bath at 80°C and quickly mix it. Stir continuously for about 2 minutes. A large amount of white flocculent precipitates, and a large amount of white flocculent is obtained. Suspension of floc precipitation; ...

Embodiment 3

[0046] 1. Prepare 100mL of agar solution with a mass percentage concentration of 4%, put it on an electric stove, stir and heat to dissolve into a translucent solution;

[0047] 2. Put the completely dissolved agar solution into a water bath to keep the temperature constant at 90°C;

[0048] 3. Prepare 100mL of a polyethylene glycol 8000 solution with a mass percentage concentration of 40%, heat it in a water bath to completely dissolve it and keep the temperature constant at 90°C;

[0049] 4. Pour the polyethylene glycol 8000 solution into the agar solution at a volume ratio of 1:0.5 to 1:3 in a water bath at 90°C and mix quickly, stirring continuously for about 2 minutes, a large amount of white flocculent precipitates, and a large amount of white flocculent is obtained. Suspension of floc precipitation; the optimal volume ratio of polyethylene glycol 6000 solution and agar solution is 1:1;

[0050] 5. Immediately stop stirring, take the suspension out of the water bath, an...

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Abstract

The invention discloses a method for separating and preparing agarose from agar by using a polyethylene glycol precipitation method. The method comprises the following steps of: 1, preparing agar solution with an appropriate concentration, and heating, stirring and dissolving the agar solution into semitransparent solution; 2, putting the agar solution into a water bath boiler to keep the temperature constant; 3, preparing PEG solution with a certain concentration, and heating the PEG solution in a water bath to ensure that the PEG solution is in a completely dissolved state and the temperature is kept constant; 4, quickly missing the agar solution with the PEG solution, and stirring the mixture continuously until a large amount of white floccule deposits appear; 5, taking the mixed solution out of the water bath boiler, and standing the mixed solution for cooling; 6, centrifuging a suspension to obtain a white deposit; 7, repeatedly washing the deposit by using a great deal of distilled water, and finally washing the deposit by using acetone; 8, drying the deposit to obtain a white agarose initial product; and 9, repeating the steps 1 to 8 to obtain refined agarose. In the method, the agarose is prepared by further purifying agar powder, so the gel strength of the agarose is improved, the electro-endosmosis of the agarose is reduced, and the method is very necessary for promoting the development of biotechnology researches.

Description

technical field [0001] The invention relates to a technique for separating and preparing agarose from agar by using a PEG method, in particular to preparing agarose with high gel strength, low sulfate radical content and low electro-endosmosis. Background technique [0002] Agar is a kind of alginate extracted from red algae plants such as agarose, river fence and seaweed. It is a long-chain polysaccharide complex composed of agarose and agar ester. Its chemical composition is polygalactose sulfate Esters are colloidal, coagulable and stable, and can be used as culture medium, ointment base and other purposes in chemical industry and medical research. Agarose in agar is the gel-forming component, the main component of which is polygalactose; while agarate is the non-gelling part, which is a complex polysaccharide with sulfate esters, glucuronic acid, and pyruvuronic acid. The existing agar containing agar ester has poor gel properties. Contents of the invention [0003] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 肖美添叶静肖笛
Owner 泉州市海川生物科技有限公司
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